Introduction to Mouse 3-IPA
3-Indolepropionic Acid (3-IPA) is a potent gut microbiota-derived metabolite with strong antioxidant and neuroprotective properties. Research on 3-IPA is crucial in studies related to metabolic health, gut-brain axis communication, inflammation, and oxidative stress. Accurate quantification is essential for understanding its biological role.
Yanda Bio’s Mouse 3-Indolepropionic Acid (3-IPA) ELISA Kit provides researchers with a reliable, precise, and affordable tool for these investigations. As a specialist manufacturer with expertise in producing over 6,000 assay targets and trusted through collaborations with more than 60 universities, we are dedicated to advancing scientific discovery through high-quality, accessible reagents. Discover the value of our cost-effective Mouse ELISA Kit production.
Why Our ELISA Kits Offer Unmatched Value vs. Premium Brands
A common question is: how can we offer such competitive pricing? The core of any ELISA kit is the antibody. High-priced kits typically utilize high-activity antibodies for superior performance.
Our Commitment: We use high-affinity antibodies sourced from premium US suppliers, identical in quality to those used in leading international brands. Our efficient manufacturing and direct-to-researcher model allow us to offer this premium component at a significantly lower cost ELISA Kit price point. You achieve the same critical sensitivity and specificity without the premium brand markup.
Dual-Format Design for Precision & Economy
We offer two optimized kit formats to suit your specific experimental needs and budget.
Tier 1: Standard Sensitivity Kit
Ideal for routine analysis of common targets where sample volume is not limited. This format employs a streamlined one-step incubation where antigen, immobilized antibody, and enzyme-labeled antibody bind simultaneously. A proprietary diluent minimizes interference from hemoglobin, lipids, and other sample matrix components, ensuring stable, low-background results. With a verified success rate exceeding 99%, this is the most low-cost ELISA Kit solution for high-quality data.
- Typical Price: ~$120 (Retail). Under $100 per kit for wholesale/volume orders.
Tier 2: Ultra-Sensitive Kit
Engineered for challenging applications involving rare targets or exceptionally precious samples. This format features a biotinylated recombinant monoclonal antibody (purity >95%) combined with a Streptavidin-HRP conjugate (activity ≥500 U/mg) for powerful signal amplification. This system directly circumvents matrix effects and background interference, maximizing the chance of first-attempt success in critical experiments.
Selection Guide:
- Choose Tier 1 for standard research, abundant samples, and optimal budget efficiency.
- Choose Tier 2 for groundbreaking research on rare analytes, limited/irreplaceable samples, or studies requiring the utmost sensitivity to detect very low concentrations.
Principle of the Assay
This kit utilizes a Sandwich-ELISA principle. Microtiter plate wells are pre-coated with an antibody specific to Mouse 3-IPA. Standards or samples are added, followed by an HRP-conjugated detection antibody. After incubation and washing to remove unbound components, TMB substrate is added. The HRP enzyme catalyzes the conversion of TMB to a blue product, which turns yellow upon addition of the stop solution. The optical density (OD) measured at 450nm is directly proportional to the concentration of 3-IPA in the sample.
Key Advantages of Choosing Yanda Bio
- Premium Components, Rational Price: Benefit from high-performance US-sourced antibodies within a cost-effective Mouse ELISA Kit structure.
- Free Assay Service: Purchase any Yanda Bio ELISA kit and qualify for FREE testing by our experts—send your samples, we deliver the results.
- Publication Reward: Cite our kit and link to our product page in your research paper, and receive a complimentary routine ELISA kit.
- 100% Quality Guarantee: We stand firmly behind our products. If performance does not meet the specifications outlined, we offer a full replacement or refund.
- Rapid Technical Support: Access expert help and receive a proposed solution within 24 hours of inquiry.
- Custom Kit Development: Require an assay not in our catalog of 6000+? Inquire about our tailored custom ELISA kit services.
Technical Specifications
- Accuracy: Correlation coefficient R of standard curve ≥ 0.9900.
- Sensitivity: Minimum detectable concentration typically < 10 pg/mL.
- Specificity: No significant cross-reactivity with analogous structures.
- Precision: Intra- and inter-assay Coefficients of Variation (CV) < 15%.
- Sample Types: Validated for serum, plasma (EDTA, citrate, heparin), cell culture supernatant, tissue homogenate.
- Storage: Store entire kit at 2-8°C. Stable for 6 months.
