Best ELISA Test Kit Manufacturer

Product Overview: Understanding Lactate in Metabolism Research

Lactate, historically considered a metabolic waste product, is now recognized as a critical signaling molecule and energy substrate involved in glycolysis, gluconeogenesis, and cellular redox balance. Accurate quantification of lactate levels is essential for research in exercise physiology, cancer metabolism (Warburg effect), sepsis, traumatic brain injury, and metabolic disorders. As a key indicator of anaerobic metabolism and tissue hypoxia, lactate measurement provides valuable insights into cellular energy status and disease progression.

Yanda Bio’s Lactate ELISA Kit employs a competitive immunoassay format optimized for sensitive and specific detection of this important metabolite. As a professional ELISA kit manufacturer with a portfolio exceeding 6,000 detectable targets and established collaborations with over 60 universities across China, we are committed to delivering high-quality, research-grade immunoassays that meet rigorous scientific standards.

---

Materials supplied

Name	96 determinations	48 determinations
Microelisa stripplate	12*8strips	12*4strips
Standard	0.3ml*6tubes	0.3ml*6tubes
Sample Diluent	6.0ml	3.0ml
HRP-Conjugate reagent	10.0ml	5.0ml
20X Wash solution	25ml	15ml
Chromogen Solution A	6.0ml	3.0ml
Chromogen Solution B	6.0ml	3.0ml
Stop Solution	6.0ml	3.0ml
Closure platemembrane	2	2
User manual	1	1
Sealed bags	1	1

Note: Standard (S0 → S5) concentration was followed by:0,3,6, 12,24,48 ng/ml

Explore Our Related Metabolite Assays:

• Customized Dihydrotestosterone (DHT) ELISA Kit
• Customized Insect α-Ketoglutaric Acid (α-KA) ELISA Kit

---

Why Choose Yanda Bio for Your Immunoassay Needs?

Expertise You Can Trust: With years of specialized experience in ELISA kit development and production, Yanda Bio has earned the trust of researchers at more than 60 universities nationwide. Our commitment to quality is reflected in every kit we produce.

EEAT-Compliant Quality Standards: Our kits are developed and validated according to rigorous protocols, ensuring Experience, Expertise, Authoritativeness, and Trustworthiness in every assay. We provide:

• Exceptional Reproducibility: Intra- and inter-assay CV < 15%
• High Specificity: No cross-reactivity with structurally similar compounds
• Proven Accuracy: Standard curve correlation coefficient (R) ≥ 0.9900

Rapid Custom Development: Need an assay for a unique target? Yanda Bio offers custom ELISA kit development services with a rapid 3-7 day turnaround—ideal for specialized research applications.

---

Two Optimized Kit Formats for Your Research Needs

To accommodate diverse experimental requirements and budget considerations, we offer this Lactate ELISA kit in two specialized formats.

Format 1: Standard High-Efficiency Kit
Designed for routine, reliable quantification of lactate in standard research settings. This format employs a streamlined one-step incubation where antigen binds simultaneously with the solid-phase antibody and enzyme-labeled antibody. A proprietary sample diluent is included to effectively minimize interference from common matrix components such as hemoglobin and lipids, ensuring stable baseline signals and optimal binding. With extensive validation demonstrating a success rate above 99%, this format represents exceptional value for most applications.

• Typical Pricing: Approximately $120 (retail). Bulk pricing available under $100 per kit.
• Special Offer: Free shipping on orders of three or more ELISA kits.

Format 2: Advanced High-Sensitivity Kit
Engineered for demanding applications where sample material is precious, limited, or where detection of very low lactate concentrations is required. This advanced format utilizes a biotinylated recombinant monoclonal antibody (purity >95%) paired with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This signal amplification system delivers superior sensitivity, directly circumvents matrix effects, and is designed to ensure success in critical, one-attempt experiments.

Selection Guide:

• Choose Format 1 for cost-effective, reliable analysis in routine monitoring and studies with sufficient sample volume
• Choose Format 2 when working with irreplaceable samples, detecting subtle changes in low-level metabolites, or requiring maximum assay robustness

---

Assay Principle: Competitive ELISA Technology

---

Sample Collection & Preparation

Proper sample handling is critical for accurate results. Please follow these guidelines:

Liquid Samples

Sample Type	Collection & Processing
Serum	Collect using sterile tubes. Allow blood to clot for 120 minutes at room temperature or overnight at 2-8°C. Centrifuge at 3000 rpm for 20 minutes at 2-8°C. Carefully collect supernatant. Store at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma	Collect using EDTA or heparin sodium as anticoagulant. Mix thoroughly. Centrifuge at 3000 rpm for 20 minutes at 2-8°C within 30 minutes of collection. Carefully collect supernatant. Store at -20°C or -80°C.
Urine, CSF, Saliva	Collect using sterile tubes. Centrifuge at 3000 rpm for 20 minutes at 2-8°C. Carefully collect supernatant. Store at -20°C or -80°C.
Cell Culture Supernatant	Collect using sterile tubes. Centrifuge at 3000 rpm for 20 minutes at 2-8°C. Carefully collect supernatant. Store at -20°C or -80°C.

Solid Samples

Sample Type	Processing Protocol
Animal Tissue	Rinse with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood. Weigh and mince tissue. Add PBS at 1:9 weight-to-volume ratio (1g tissue to 9mL PBS). Homogenize on ice, then centrifuge at 5000×g for 10 minutes at 2-8°C. Collect supernatant.
Adherent Cells	Wash with pre-chilled PBS, detach with trypsin, and collect by centrifugation (1000×g, 5 min). Wash cells 3 times with cold PBS. Resuspend at 1×10⁷ cells/mL in cold PBS. Lyse by sonication. Centrifuge at 3000 rpm for 20 minutes at 2-8°C. Collect supernatant.
Suspension Cells	Collect by centrifugation, wash 3 times with cold PBS, then follow same procedure as adherent cells.
Swab Samples	Dissolve swab head in 2 mL PBS (pH 7.2-7.4), shake well. Remove swab and squeeze dry. Centrifuge at 2000-3000 rpm for 20 minutes at 2-8°C. Collect supernatant.

Important Sample Notes

• Extract samples as soon as possible after collection. Perform assays promptly after extraction.
• For short-term storage (up to 6 days), samples can be stored at 2-8°C.
• For long-term storage, freeze at -20°C (≤1 month) or -80°C (≤2 months). Avoid repeated freeze-thaw cycles.
• If precipitate forms during storage, centrifuge again before testing.
• Do not use samples containing sodium azide (NaN₃), as it inhibits horseradish peroxidase (HRP) activity.

---

Materials Required But Not Provided

1. Microplate reader capable of measuring absorbance at 450 nm
2. Precision pipettes and tips (0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL)
3. 37°C incubator or water bath

---

Important Notes for Assay Procedure

1. Store the kit at 2-8°C. Allow all reagents to equilibrate to room temperature for 20 minutes before use. Concentrated wash buffer may crystallize; this is normal. Dissolve crystals by warming in a water bath.
2. Return unused strips to the resealable bag with desiccant and store properly at 2-8°C. Use as soon as possible.
3. The S0 standard (zero concentration) serves as a negative control/blank. Note that samples are pre-diluted 2-fold during the procedure; multiply results by 2 to obtain actual concentrations.
4. Strictly adhere to the incubation times, volumes, and sequence specified in the protocol.
5. Mix all liquid components thoroughly before use.

---

Reagent Preparation

20× Wash Buffer Dilution: Dilute 1 part 20× wash buffer with 19 parts distilled water to prepare 1× working wash buffer.

---

Washing Method

Manual Washing: Aspirate liquid from wells, fill each well with wash buffer, allow to stand for 1 minute, then aspirate. Invert plate and tap dry on absorbent paper. Repeat 5 times.

Automated Plate Washer: Inject 350 μL of wash buffer per well, allow to soak for 1 minute, and wash 5 times.

---

Assay Procedure

1. Remove the required number of strips from the aluminum foil bag after 20 minutes of room temperature equilibration. Reseal unused strips and return to 4°C.
2. Set up standard wells and sample wells. Add 50 μL of each standard concentration to the standard wells.
3. For sample wells: Add 25 μL of test sample followed by 25 μL of sample diluent.
4. Add 50 μL of HRP-labeled competitive antigen (tracer) to both standard and sample wells. Seal with a plate cover and incubate at 37°C for 60 minutes.
5. Aspirate liquid, tap dry on absorbent paper, fill each well with wash buffer, let stand for 1 minute, aspirate, and tap dry. Repeat washing 5 times (automated washer acceptable).
6. Add 50 μL of Substrate A and 50 μL of Substrate B to each well. Incubate at 37°C in the dark for 15 minutes.
7. Add 50 μL of stop solution to each well. Measure the OD at 450 nm within 15 minutes.

---

Data Analysis

This competitive ELISA requires calculation of percent binding for accurate quantification.

Calculation Steps:

1. Read OD Values: Record absorbance at 450 nm within 15 minutes of adding stop solution.
2. Calculate Percent Binding:

• Let B0 = OD of S0 standard (zero concentration)
• Let B = OD of each standard or sample
• Let NSB = Non-specific binding (if applicable)
• Percent binding = (B / B0) × 100%

3. Construct Standard Curve:

• Plot standard concentrations (x-axis, log scale) against percent binding (y-axis, linear scale) using logit-log or 4-parameter logistic (4PL) curve fitting.
• Calculate logit values: logit = ln[B/B0 / (1 – B/B0)]

4. Determine Sample Concentrations:

• Calculate percent binding for each sample
• Interpolate concentration from the standard curve
• Multiply by the dilution factor (2×) to obtain actual sample concentration

Automated Analysis: Use ELISA data analysis software with logit-log or 4PL models for automatic curve fitting and concentration calculation.

---

Kit Performance Specifications

Parameter	Performance
Accuracy	Standard curve linear regression coefficient (R) ≥ 0.9900
Sensitivity	Minimum detectable concentration < 5 ng/mL
Detection Range	50 – 2000 ng/mL
Specificity	No significant cross-reactivity with other organic acids or soluble structural analogs
Intra-assay CV	< 15%
Inter-assay CV	< 15%
Storage	2-8°C, protected from light and moisture
Shelf Life	6 months

---

Why Yanda Bio Delivers EEAT-Compliant Quality

Principle	Our Commitment
Experience	Over 6,000 detectable factors developed; 60+ university collaborations; years of specialized expertise in immunoassay manufacturing.
Expertise	Specialized R&D team; proprietary ELISA optimization protocols; dual-format kit design for research flexibility.
Authoritativeness	Validated by academic research partners; publications citing our kits; ISO-aligned manufacturing standards.
Trustworthiness	Quality guarantee; custom service with 3-7 day turnaround; transparent specifications; reliable technical support.

---

Special Offer

Purchase any three ELISA kits from Yanda Bio and enjoy free shipping. Combine our Lactate kit with our Pyruvate and Glucose kits to build a comprehensive metabolism research panel at exceptional value.

---

Comparison with Premium International Brands

Yanda Bio’s ELISA kits deliver performance comparable to leading international brands at a fraction of the cost. Our efficient manufacturing process and direct-to-researcher business model eliminate unnecessary markups, allowing us to offer professional-grade immunoassays at highly competitive prices. Whether you choose our standard format for routine analysis or the high-sensitivity format for demanding applications, you receive exceptional value without compromising on data quality.

---

Disclaimer

1. This product is for research use only. It is not intended for clinical diagnosis or human use. The user assumes all responsibility for any consequences arising from misuse.
2. Strict adherence to the protocol is required. Yanda Bio is not responsible for results obtained from deviations from the specified procedure.

---

Ordering & Contact Information

To order this Lactate ELISA Kit, or to inquire about volume discounts, format selection guidance, and custom services, please contact our sales team.