Product Overview: Understanding Interferon γ in Equine Research
Interferon γ (IFN-γ) is a critical Th1-type cytokine produced primarily by activated T cells, natural killer (NK) cells, and macrophages. It plays a central role in innate and adaptive immunity, mediating host defense against intracellular pathogens, regulating inflammatory responses, and orchestrating immune cell activation. In equine research, accurate quantification of Horse IFN-γ is essential for studies on infectious diseases (such as equine influenza, strangles, and equine herpesvirus), vaccine efficacy assessment, immune-mediated disorders, and the pathophysiology of conditions like recurrent airway obstruction (RAO) and insect bite hypersensitivity.
Yanda Bio’s Horse Interferon γ (IFN-γ) ELISA Kit employs a sandwich immunoassay format optimized for sensitive and specific detection of this key cytokine. As a professional ELISA kit manufacturer with a portfolio exceeding 6,000 detectable targets and established collaborations with over 60 universities across China, we combine deep immunology expertise with rigorous quality standards to support equine health research.
related Horse Elisa Kit:
- Horse C-Reactive Protein (CRP) ELISA Kit — For inflammation and acute phase response monitoring
- Horse 5-Hydroxytryptamine (5-HT) ELISA Kit — For neurotransmitter and stress response research
Why Choose Yanda Bio for Your Equine Immunoassays?
EEAT-Compliant Quality Standards
Our commitment to Experience, Expertise, Authoritativeness, and Trustworthiness ensures that every kit meets the highest research standards for cytokine quantification.
| Principle | Our Commitment |
|---|---|
| Experience | Over 6,000 detectable factors developed; 60+ university collaborations; specialized expertise in equine immunoassay development and validation. |
| Expertise | Proprietary antibody pairs optimized for horse cytokines; sandwich ELISA format ensures high specificity and sensitivity. |
| Authoritativeness | Validated by academic research partners; ISO-aligned manufacturing standards; rigorous quality control at every production stage. |
| Trustworthiness | Rapid custom service with 3-7 day turnaround; transparent specifications; unconditional quality guarantee. |
Superior Quality Metrics
- Intra-assay CV < 5% — Exceptional within-run precision
- Inter-assay CV < 8% — Outstanding run-to-run consistency
- High Specificity — Minimal cross-reactivity with other equine cytokines
Kit Components: What’s Included
Our Horse IFN-γ ELISA Kit is available in both 96-well and 48-well configurations to accommodate different throughput requirements.
| Component | 96-Well Configuration | 48-Well Configuration |
|---|---|---|
| Microplate (Pre-coated) | 8 wells × 12 strips | 8 wells × 6 strips |
| Standards | 0.3 mL × 6 tubes | 0.3 mL × 6 tubes |
| Sample Diluent | 6 mL | 3 mL |
| HRP-Conjugated Detection Antibody | 6 mL | 3 mL |
| 20× Wash Buffer | 25 mL | 15 mL |
| Substrate A | 6 mL | 3 mL |
| Substrate B | 6 mL | 3 mL |
| Stop Solution | 6 mL | 3 mL |
| Plate Sealer | 2 sheets | 2 sheets |
| Manual | 1 copy | 1 copy |
Assay Principle: Sandwich ELISA Technology
This kit employs a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) principle. A monoclonal antibody specific for Horse Interferon γ (IFN-γ) is pre-coated onto the microplate. Standards and samples are pipetted into the wells, allowing IFN-γ to bind to the immobilized antibody. After washing to remove unbound substances, a Horseradish Peroxidase (HRP)-conjugated detection antibody is added, forming an antibody-antigen-antibody “sandwich” complex. Following a second wash step, TMB substrate is added. The HRP enzyme catalyzes the conversion of TMB to a blue product, which turns yellow upon acidification with the stop solution. The optical density (OD) measured at 450 nm is directly proportional to the concentration of IFN-γ in the sample.
Sample Dilution Note: Samples are pre-diluted 5-fold during the procedure; multiply results by 5 to obtain actual concentrations.
Sample Collection & Preparation
Proper sample handling is critical for accurate cytokine quantification. Please follow these guidelines:
| Sample Type | Collection & Processing |
|---|---|
| Serum | Use pyrogen/endotoxin-free tubes. Avoid cell stimulation. Collect blood, centrifuge at 3000 rpm for 10 minutes to carefully separate serum from red blood cells. |
| Plasma | Collect using EDTA, citrate, or heparin anticoagulants. Centrifuge at 3000 rpm for 30 minutes and collect the supernatant. |
| Cell Supernatant | Centrifuge at 3000 rpm for 10 minutes to remove particles and polymers. |
| Tissue Homogenate | Homogenize tissue with an appropriate volume of physiological saline. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant. |
| Storage | If samples are not tested immediately, aliquot and store at -20°C. Avoid repeated freeze-thaw cycles. Thaw at room temperature and ensure complete mixing before use. |
Materials Required But Not Provided
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes and tips (0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL)
- 37°C incubator or water bath
Important Notes for Assay Procedure
- Store the kit at 2-8°C. Allow all reagents to equilibrate to room temperature for 20 minutes before use. Concentrated wash buffer may crystallize; this is normal. Dissolve crystals by warming in a water bath.
- Return unused strips to the resealable bag with desiccant and store properly at 2-8°C. Use as soon as possible.
- The S0 standard (zero concentration) serves as a negative control/blank. Note that samples are pre-diluted 5-fold during the procedure; multiply results by 5 to obtain actual concentrations.
- Strictly adhere to the incubation times, volumes, and sequence specified in the protocol.
- Mix all liquid components thoroughly before use.
Reagent Preparation
20× Wash Buffer Dilution: Dilute 1 part 20× wash buffer with 19 parts distilled water to prepare 1× working wash buffer.
Washing Method
Manual Washing: Aspirate liquid from wells, fill each well with wash buffer, allow to stand for 1 minute, then aspirate. Invert plate and tap dry on absorbent paper. Repeat 5 times.
Automated Plate Washer: Inject 350 μL of wash buffer per well, allow to soak for 1 minute, and wash 5 times.
Assay Procedure
- Remove the required number of strips from the aluminum foil bag after 20 minutes of room temperature equilibration. Reseal unused strips and return to 4°C.
- Set up standard wells and sample wells. Add 50 μL of each standard concentration to the standard wells.
- For sample wells: Add 10 μL of test sample followed by 40 μL of sample diluent. Leave blank wells empty.
- Except for blank wells, add 100 μL of HRP-conjugated detection antibody to all standard and sample wells. Seal with a plate cover and incubate at 37°C for 60 minutes.
- Aspirate liquid, tap dry on absorbent paper, fill each well with wash buffer, let stand for 1 minute, aspirate, and tap dry. Repeat washing 5 times (automated washer acceptable).
- Add 50 μL of Substrate A and 50 μL of Substrate B to each well. Incubate at 37°C in the dark for 15 minutes.
- Add 50 μL of stop solution to each well. Measure the OD at 450 nm within 15 minutes.
Data Analysis
Create a standard curve by plotting the OD values (y-axis) against the standard concentrations (x-axis) in Excel or suitable software. A linear regression curve (R ≥ 0.9900) provides the best fit. Calculate sample concentrations from the curve equation, multiplying by the dilution factor (5×) as indicated.
Kit Performance Specifications
| Parameter | Performance |
|---|---|
| Accuracy | Standard curve linear regression coefficient (R) ≥ 0.9900 |
| Sensitivity | Minimum detectable concentration < 1.0 pg/mL |
| Detection Range | 1.0 – 40 pg/mL |
| Specificity | No significant cross-reactivity with other equine cytokines or soluble structural analogs |
| Intra-assay CV | < 5% |
| Inter-assay CV | < 8% |
| Storage | 2-8°C, protected from light and moisture |
| Shelf Life | 6 months |
Customization Options
Yanda Bio offers custom ELISA kit development services with a rapid 3-7 day turnaround for specialized research applications:
| Custom Parameter | Available Options |
|---|---|
| Target Species | Horse, or adaptation to other equine-related species |
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernatants, bronchoalveolar lavage fluid |
| Detection Range | Adjustable based on expected physiological levels |
| Sensitivity | Optimized for your specific application |
| Kit Format | Standard or high-sensitivity options |
Related Products
Complete Your Equine Health Research Panel:
- Horse C-Reactive Protein (CRP) ELISA Kit — For acute phase inflammation monitoring. Combine with IFN-γ to assess the relationship between Th1 immune activation and systemic inflammatory status.
- Horse 5-Hydroxytryptamine (5-HT) ELISA Kit — For neurotransmitter and stress response studies. Investigate the interaction between immune activation (IFN-γ) and neuroendocrine responses (5-HT) in equine models.
Special Offer
Purchase any three ELISA kits from Yanda Bio and enjoy free shipping. Combine our Horse IFN-γ kit with our Horse CRP and Horse 5-HT kits to build a comprehensive equine immune and stress research panel at exceptional value.
Comparison with Premium International Brands
Yanda Bio’s ELISA kits deliver performance comparable to leading international brands at a fraction of the cost—often 40-60% less than major commercial alternatives. Our efficient manufacturing process and direct-to-researcher business model eliminate unnecessary markups, allowing us to offer professional-grade cytokine immunoassays at highly competitive prices. With superior precision metrics (CV < 5% intra-assay, CV < 8% inter-assay) and 3-7 day custom development capability, we provide exceptional value without compromising on data quality.
Disclaimer
Strict adherence to the protocol is required. Yanda Bio is not responsible for results obtained from deviations from the specified procedure. This product is for research use only. It is not intended for clinical diagnosis or human use. The user assumes all responsibility for any consequences arising from misuse.
Ordering & Contact Information
To order this Horse Interferon γ (IFN-γ) ELISA Kit, or to inquire about volume discounts, custom development, and format selection guidance, please contact our sales team.
