Key Features & Benefits
- High-Sensitivity Detection: Leverages a >95% pure biotinylated recombinant monoclonal antibody and a high-activity streptavidin-HRP conjugate (≥500 U/mg) for superior signal amplification and low background.
- Excellent Specificity: Minimal cross-reactivity ensures accurate measurement of MNAM without interference from structurally similar metabolites.
- Optimized One-Step Protocol: Streamlined procedure reduces total assay time and pipetting steps, enhancing laboratory throughput and efficiency.
- Cost-Effective for Any Scale: Obtain premium quality at an accessible retail price of $120. For high-volume studies, our bulk pricing drops below $100, offering exceptional value.
- Comprehensive Support Services: We offer custom ELISA kit development to meet unique project needs and a complimentary sample testing service to validate assay performance with your specific samples.
Enhanced Assay Principle
This kit employs a sensitive one-step sandwich enzyme-linked immunosorbent assay (ELISA) with signal amplification:
- Capture: A specific antibody for 1-Methylnicotinamide (MNAM) is pre-coated onto the microplate wells.
- Complex Formation: Samples or standards are incubated simultaneously with a biotinylated detection antibody. The target MNAM is captured, forming an antibody-analyte-antibody “sandwich” complex.
- Signal Amplification: After washing, a Streptavidin-Horseradish Peroxidase (SA-HRP) conjugate is added, which binds with high affinity to the biotin, creating an amplified detection system.
- Detection & Quantification: Following another wash, TMB substrate is added. HRP catalyzes the conversion of TMB to a blue product, which turns yellow upon addition of the stop solution. The absorbance at 450 nm is measured. The intensity of the color is directly proportional to the concentration of MNAM in the sample.
Kit Components & Specifications
Complete Kit Contents (96T / 48T Configurations)
- Pre-coated Microplate (8 strips / 4 strips)
- Standards (0.3mL x 6 tubes: 0, 5, 10, 20, 40, 80 ng/mL)
- Sample Diluent (6mL / 3mL)
- Biotinylated Detection Antibody (10mL / 5mL)
- Streptavidin-HRP Conjugate (10mL / 5mL)
- 20x Wash Buffer (25mL / 15mL)
- Substrate A & B (6mL each / 3mL each)
- Stop Solution (6mL / 3mL)
- Plate Sealers, Detailed Manual, Storage Bag
Performance Characteristics
| Parameter | Specification |
|---|---|
| Accuracy (Linearity) | Correlation coefficient R ≥ 0.9900 |
| Sensitivity | Minimum Detectable Concentration < 1.0 ng/mL |
| Detection Range | 1.0 ng/mL – 80 ng/mL |
| Specificity | No significant cross-reactivity observed |
| Precision | Intra- & Inter-assay CV < 15% |
| Storage | 2-8°C, protected from light and moisture |
| Shelf Life | 6 months |
Sample Collection & Preparation
- Serum/Plasma: Collect using standard tubes. Centrifuge at 3000×g for 10-30 minutes to obtain clear supernatant.
- Urine/Cell Culture Supernatant: Centrifuge at 3000×g for 10 minutes to remove particulates.
- Storage: Aliquot and store samples at -20°C for long-term preservation. Avoid repeated freeze-thaw cycles. Thaw completely and mix gently before use.
Ordering & Service Options
Yanda Bio provides flexible solutions to fit your research needs and budget.
| Option | Description | Best For |
|---|---|---|
| Retail Purchase | Single kit at $120. | Individual researchers or pilot studies. |
| Bulk Purchase | Multiple kits, pricing starts under $100. | Core facilities, labs, and long-term projects. |
| Custom Kit Development | Tailor sensitivity, range, or sample compatibility. | Unique assays, novel applications, or specific matrix validation. |
| Free Testing Service | Send your samples for a complimentary assay run & report. | Method verification or labs without immediate ELISA capability. |
Contact our sales team today for a custom bulk quote, to discuss customization, or to arrange your free sample testing service.
Research Applications
This kit is for Research Use Only (RUO). It is ideally suited for:
- NAD+ Metabolism Studies: Investigating the nicotinamide pathway and NNMT enzyme activity.
- Metabolic & Cardiovascular Research: Exploring the role of MNAM as a potential vasoactive agent and metabolic biomarker.
- Aging & Neurological Research: Studying metabolic changes associated with aging and neurodegenerative conditions.
- Nutritional Science: Assessing niacin (Vitamin B3) status and metabolic flux.
Frequently Asked Questions (FAQ)
Q: What is the significance of measuring 1-Methylnicotinamide (MNAM)?
A: MNAM is a key metabolite reflecting NNMT activity and NAD+ metabolism. Its levels are implicated in various metabolic conditions, making it a valuable biomarker for related research.
Q: Why does the kit use a biotin-streptavidin system?
A: The biotin-streptavidin interaction is one of the strongest in nature, allowing for significant signal amplification. This results in higher sensitivity, lower detection limits, and improved assay robustness compared to conventional direct-HRP conjugates.
Q: How do I switch from retail to bulk pricing?
A: Simply contact us directly. Our bulk pricing (under $100 per kit) is automatically applied for orders of multiple kits. We provide customized quotes based on your exact volume.
Q: Is technical support available?
A: Yes. We provide comprehensive product manuals and dedicated email support to assist with assay setup, troubleshooting, and data interpretation.
Why Choose Yanda Bio?
At Yanda Bio, we empower scientific discovery with reliable, high-performance assay kits at transparent prices. Our Human 1-Methylnicotinamide (MNAM) ELISA Kit combines cutting-edge detection technology with exceptional value, backed by our commitment to flexible customization and expert support. Choose Yanda Bio for precise, affordable, and reliable solutions for your metabolic research.
To purchase the Human 1-Methylnicotinamide (MNAM) ELISA Kit for sale, request a quote, or learn more about our services, contact us now.
