Choose Your Optimal Assay Configuration
To cater to diverse research needs and budgets, we offer two optimized kit tiers. Free sample testing is included with the purchase of any tier.
FeatureTier 1: Standard cGAMP ELISA KitTier 2: High-Sensitivity cGAMP ELISA Kit
Core Technology Optimized one-step sandwich ELISA with specialized diluent. Biotinylated recombinant mAb (>95% pure) + Streptavidin-HRP (≥500 U/mg).
Key Advantage: Cost-Effective & Robust. Features a simultaneous single-step incubation and a proprietary diluent that reduces background interference from hemoglobin and lipids, ensuring stable performance. Maximized Sensitivity & Specificity. The advanced amplification system directly circumvents matrix effects, offering superior signal-to-noise for detecting very low abundance targets.
Ideal For Routine quantification, high-throughput screening, and studies where cGAMP level was expected to be within a detectable range. Demanding applications requiring ultimate sensitivity, working with limited sample volumes, or analyzing samples with high background interference.
Expert Outsourced Testing Service: Your Data, Delivered
We understand the value of your time and samples. Our professional laboratory offers a seamless testing service:
- Rapid Turnaround: We initiate your experiment within 24 hours of sample receipt, delivering a comprehensive data package in as few as 5 business days.
- Complete Reporting: You receive a detailed data pack containing raw OD values, calculated concentration tables, and a standard curve graph.
Our Unwavering Commitment to Your Research Success
We back our products and services with clear, actionable commitments that build trust:
- Free Kit with Publication: Cite this Human 2′,3′-cGAMP ELISA Kit in your paper with a link to our product page, and we will send you a free standard ELISA kit (up to $120 value) as a token of our appreciation.
- 100% Satisfaction Guarantee: If any product does not meet its published specifications, we offer an unconditional replacement or refund.
- Responsive Support: Our team commits to providing a proposed resolution within 24 hours of any technical or service inquiry.
- Guardians of Your Science: We treat every sample with the utmost care, recognizing its precious value and the critical importance of your research outcomes.
Assay Principle & Key Specifications
Principle: This kit utilizes a one-step sandwich ELISA. The target 2′,3′-cGAMP was captured between a pre-coated antibody and an HRP-conjugated detection antibody during a single incubation. A TMB substrate reaction, measured at 450 nm, produces a signal proportional to the cGAMP concentration.
Performance Specifications:
- Sensitivity: < 1.0 ng/mL (or equivalent).
- Detection Range: 1.0 – 200 ng/mL.
- Accuracy (Linearity): Correlation Coefficient R ≥ 0.9900.
- Precision: Intra- & Inter-assay CV < 15%.
- Specificity: No significant cross-reactivity with analogous nucleotides.
- Storage: 2-8°C; stable for 6 months.
Sample Preparation: For serum/plasma, a 1:5 dilution with the provided buffer is required. Cell and tissue lysates should be prepared using appropriate extraction buffers. Clarify all samples by centrifugation (3000×g for 10 mins) and store at -20°C in single-use aliquots.
Simplified Workflow Overview
- Add: Pipette 50 µL of standards or pre-diluted samples into the pre-coated wells.
- Incubate: Add 100 µL of HRP-conjugated Detection Antibody. Incubate at 37°C for 60 minutes.
- Wash: Aspirate and wash the plate 5 times with diluted wash buffer.
- Develop: Add 50 µL each of Substrate A and B. Incubate at 37°C for 15 minutes in the dark.
- Stop & Read: Add 50 µL Stop Solution. Measure absorbance at 450 nm within 15 minutes.
Pricing, Services & Contact
OptionDescriptionLead Time / Price
Tier 1: Standard Kit (Purchase) Reliable, cost-effective in-lab solution. Retail: $120. Bulk: < $100 (quote required).
Tier 2: High-Sensitivity Kit (Purchase) Premium performance for low-abundance targets. Contact for a competitive manufacturer’s price.
Expert Testing Service: We run your assay and deliver the full data report. ~5 business days. Included free with kit purchase or quoted separately.
Custom ELISA Development: Tailored solutions for unique requirements. Project-based quotation.
Ready to advance your research on the cGAS-STING pathway? Contact Yanda Bio to order your kit, inquire about our testing service, or request a custom quote.
Applications & Frequently Asked Questions (FAQ)
Primary Research Applications (RUO): Innate immunity, cancer immunotherapy (checkpoint inhibitors, vaccine adjuvants), viral infection response, autoimmune disease mechanisms, and nucleic acid sensing research.
Q: What is the main difference between the two tiers?
A: The core difference is the detection system. Tier 1 uses an optimized standard format ideal for most applications. Tier 2 employs a biotin-streptavidin amplification system, providing higher sensitivity and better resistance to matrix interference, suited for the most challenging samples or low-concentration targets.
Q: How do I claim the free kit for publication?
A: Upon acceptance/publication of your article that cites our Human 2′,3′-cGAMP ELISA Kit (Yanda Bio) and includes a link to our product page, send us the citation details. We will ship your complimentary kit.
Q: Can I use this kit for mouse or rat samples?
A: This kit is validated for the human target. Cross-reactivity with rodent cGAMP is not guaranteed and should be determined by the researcher. We offer custom development services for species-specific assays.
Q: Are you the actual manufacturer?
A: Yes. We are the direct source manufacturer, which allows us to control quality, ensure competitive pricing, and provide expert technical support directly from our professional labs.
Manufacturer’s Note
This product is for Research Use Only (RUO). It is not intended for diagnostic, therapeutic, or clinical procedures. Users are responsible for validating the kit’s suitability for their specific experimental system.
