Human CC Chemokine Ligand 12 (CCL12/MCP-5) ELISA Kit
Yanda Bio’s Human CC Chemokine Ligand 12 (CCL12) ELISA Kit is a validated, high-performance assay for the precise quantification of CCL12 (also known as MCP-5) in serum, plasma, cell culture supernatants, and tissue homogenates. As a key member of the CC chemokine family, CCL12 plays a critical role in recruiting monocytes, lymphocytes, and other immune cells to sites of inflammation, making it a vital biomarker for research in immunology, autoimmune diseases, cancer biology, and chronic inflammatory conditions.
As a certified factory-direct manufacturer with in-house R&D laboratories, we offer premium quality at transparent prices. This kit is available at a retail price of $120, with bulk pricing under $100. Our extensive portfolio features over 6,000 detection targets across immunology, oncology, neuroscience, metabolism, and infectious disease, supported by long-term collaborations with more than 70 universities worldwide.
Choose Your Optimal Assay Format
To meet diverse research needs, we provide two optimized kit tiers. Purchasing either tier includes our complimentary expert testing service.
| Feature | Tier 1: Standard CCL12 ELISA Kit | Tier 2: High-Sensitivity CCL12 ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with proprietary dilution buffer. | Biotinylated recombinant mAb (>95% pure) + Streptavidin-HRP (≥500 U/mg). |
| Key Advantage | Excellent Value & Reliability. Uses a simultaneous incubation step and a specialized diluent to minimize background interference from hemoglobin and lipids, ensuring robust performance. | Ultimate Sensitivity & Specificity. The advanced biotin-streptavidin system effectively neutralizes matrix effects, delivering superior signal-to-noise for low-abundance targets. |
| Ideal For | Routine analysis in standard immunoassay workflows. | High-precision studies, low-concentration samples, or complex matrices. |
Included Expert Testing Service: Fast, Reliable Data
Leverage our professional laboratory to save time and ensure optimal results.
- Rapid Turnaround: We begin analysis within 24 hours of sample receipt, delivering a comprehensive data package in ~5 business days.
- Complete Report: Receive raw OD values, calculated concentration tables, and standard curve graphs.
- Validation Support: Ideal for confirming assay performance with your specific sample types.
Our Commitment to Your Research Success
We build trust through actionable commitments:
- Publication Reward: Cite this CCL12 ELISA Kit with a link to our product page in your publication, and receive a free standard ELISA kit (up to $120 value).
- 100% Satisfaction Guarantee: We offer an unconditional replacement or refund if a product does not meet its specifications.
- 24-Hour Support Pledge: Our team provides a proposed resolution within 24 hours of any inquiry.
- Integrity in Science: We handle every sample with the utmost care, safeguarding your research integrity.
Why Choose Yanda Bio?
- Comprehensive Portfolio & Assured Quality: Access 6,000+ validated assays with stringent QC.
- High Sensitivity & Superior Stability: Achieve reliable detection with excellent batch-to-batch consistency.
- Simple Protocol & Rapid Workflow: User-friendly design saves time and reduces operational complexity.
- Competitive Pricing & High Value: Direct-manufacturer pricing ensures the best cost-performance ratio.
- Ample Stock & Fast Delivery: Efficient logistics ensure prompt delivery.
- Complete Product Support: Free access to latest quotes, protocols, applications, and manuals.
- Comprehensive After-Sales Service: Dedicated support from order to data analysis.
Assay Principle & Specifications
Principle: This one-step sandwich ELISA captures CCL12 between a pre-coated antibody and an HRP-conjugated detection antibody. TMB substrate produces a colorimetric signal measured at 450 nm, proportional to CCL12 concentration.
Performance Specifications:
- Sensitivity: < 10.0 pg/mL.
- Detection Range: 10.0 – 800 pg/mL.
- Accuracy (Linearity): R ≥ 0.9900.
- Precision: Intra- & Inter-assay CV < 15%.
- Specificity: No significant cross-reactivity.
- Storage: 2-8°C; 6-month shelf life.
Sample Preparation: Process serum, plasma, or cell culture supernatant by centrifugation (3000×g for 10-30 mins). Aliquot and store samples at -20°C; avoid repeated freeze-thaw cycles. A 1:5 dilution is recommended for serum/plasma.
Streamlined Assay Workflow
- Add 50 µL of standards or diluted samples to wells.
- Add 100 µL HRP-conjugated Detection Antibody.
- Incubate at 37°C for 60 min.
- Wash plate 5 times.
- Add 50 µL each of Substrate A & B. Incubate at 37°C for 15 min (dark).
- Add 50 µL Stop Solution. Read OD at 450 nm within 15 min.
Calculation: Plot the standard curve (0, X, Y… pg/mL) and interpolate sample values. Multiply results by the dilution factor.
Pricing & Services
| Option | Description | Details |
|---|---|---|
| Tier 1: Standard Kit | Cost-effective, reliable in-lab solution. | Retail: $120. Bulk: < $100. |
| Tier 2: High-Sensitivity Kit | Premium performance for maximum sensitivity. | Contact for manufacturer price. |
| Expert Testing Service | Full assay execution and data report. | ~5-day turnaround. Free with purchase. |
| Custom ELISA Development | Tailored solutions from our vast portfolio. | Project-based quote. |
To order your Human CCL12 ELISA Kit or inquire about our 6,000+ assays, contact Yanda Bio, your trusted direct supplier in China.
Applications & FAQ
Research Applications (RUO): Inflammatory disease research (arthritis, atherosclerosis), cancer microenvironment studies, autoimmune disorder mechanisms, monocyte/macrophage migration studies, and immunotoxicology.
Q: What is the difference between CCL12 and MCP-1?
A: While both are monocyte chemoattractants, CCL12 (MCP-5) and CCL2 (MCP-1) are distinct genes with specific receptor interactions and biological contexts. Our kit is specific for human CCL12.
Q: How do I claim the free kit for publication?
A: Upon publication of your article citing “Human CCL12 ELISA Kit (Yanda Bio)” with a link to our product page, send us the citation to claim your complimentary kit.
Q: How can I search your 6,000+ assay portfolio?
A: Contact our technical support with your target name or research area. We provide rapid availability checks and recommendations.
Q: How do I buy this ELISA kit from China?
A: As the direct manufacturer in China, you can buy this Human CCL12 ELISA Kit online by contacting Yanda Bio. We handle all export documentation and global shipping.
Manufacturer’s Note
This product is for Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Users are responsible for determining the kit’s suitability for their specific applications.
