Human IFN-γ R1 ELISA Kit Materials supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Key Features & Benefits
- High Sensitivity & Wide Range: Achieve accurate detection across a broad dynamic range, ideal for measuring both basal and elevated levels of IFN γR1.
- Streamlined One-Step Protocol: Save valuable time and reduce pipetting steps with our optimized one-step sandwich ELISA procedure.
- Cost-Effective Bulk Pricing: Our standard wholesale pricing starts below $100 per kit, with additional discounts available for larger volume purchases.
- Customizable to Your Needs: We offer full custom service for this kit. Tailor the sample type compatibility (e.g., specific biofluids) or adjust the assay sensitivity and detection range to perfectly fit your experimental requirements.
- Rigorous Validation: Excellent intra and inter-assay precision ensures reproducible and reliable results batch after batch.
Detection Principle: One-Step Sandwich ELISA
This Human IFN γR1 ELISA Kit utilizes a highly efficient one-step sandwich enzyme-linked immunosorbent assay (ELISA) principle:
- Capture: Microplate wells are pre-coated with a highly specific antibody against Human IFN γR1.
- Binding & Detection: Samples or standards are simultaneously incubated with the HRP-conjugated detection antibody. This single-step incubation allows the target IFN γR1 to be captured and detected concurrently, forming an antibody-antigen-antibody “sandwich” complex.
- Wash & Develop: After a thorough wash to remove unbound material, the substrate solution (TMB) is added. The horseradish peroxidase (HRP) catalyzes the conversion of TMB to a blue product.
- Stop & Quantify: The reaction is stopped with an acid solution, turning the final product yellow. The absorbance (Optical Density, OD) is measured at 450 nm using a microplate reader. The intensity of the yellow color is directly proportional to the concentration of Human IFN γR1 present in the original sample.
Intended Applications
This kit is for research use only (RUO). Key applications include:
- Immunology Research: Studying IFN-γ signaling pathways and immune cell regulation.
- Disease Mechanism Studies: Investigating the role of IFN γR1 in chronic infections, autoimmune diseases (e.g., rheumatoid arthritis), and immunodeficiencies.
- Biomarker Analysis: Evaluating soluble IFN γR1 levels as a potential biomarker in various clinical conditions.
- Therapeutic Development: Monitoring receptor levels during the development and testing of immunomodulatory drugs or biologics.
*Delivery times may vary based on customization and destination.
Ready for a bespoke solution? Our expert team can customize this Human IFN-gamma R1 ELISA Kit for you. Whether you need to adapt the kit for a unique sample matrix or adjust the sensitivity (lower/higher detection limit), we can collaborate to meet your exact specifications. Contact our technical sales team to discuss your custom project.
Frequently Asked Questions (FAQ)
Q: What is the main advantage of the one-step assay format?
A: The one-step sandwich ELISA significantly reduces total hands-on time and simplifies the procedure by combining the sample and detection antibody incubation steps, increasing throughput while maintaining high performance.
Q: How does your pricing compare to other suppliers?
A: Yanda Bio is committed to providing premium quality reagents at accessible prices. Our direct-to-lab model allows us to offer consistent bulk pricing under $100, delivering exceptional value, especially for core labs and long-term studies.
Q: What level of customization do you offer?
A: We offer extensive customization for this kit, including but not limited to: modifying the sample dilution buffers for specific fluids, adjusting the standard curve range, and providing custom-matched components for specialized applications.
Q: Is this kit suitable for diagnostic use?
A: No. This product is developed and validated for Research Use Only (RUO). It is not intended for diagnostic, therapeutic, or clinical procedures.
Why Partner with Yanda Bio?
At Yanda Bio, we empower your research with reliable, high-performance assay kits that won’t strain your budget. Our Human IFN γR1 ELISA Test Kit exemplifies this commitment, offering a perfect blend of scientific rigor, operational convenience, and cost efficiency. Combined with our flexible custom service options, we ensure you have the right tool for your specific research questions.
Contact us today to place an order, request a bulk quotation, or initiate a conversation about your custom kit requirements.
