Ordering, Customization & Service Options
Option 1: Purchase Standard Kit
- Pricing: Contact for an exact quote. Our competitive structure ensures bulk pricing for human ELISA kits remains under $100.
- Formats: 48T or 96T.
Option 2: Custom Kit Development
- Service: Need a different assay range, sensitivity, or sample type validation? Our R&D team can tailor a kit to your precise specifications.
Option 3: Free Sample Testing Service
- Service: Unsure if the kit fits your application? Send us your samples. We will perform the assay and provide you with a detailed data report at no cost.
Contact our sales team today for pricing, to discuss customization, or to arrange your complimentary sample analysis.
Product Advantages & Key Features
- Target-Specific Accuracy: Precisely quantifies human IP3 with high specificity, minimizing interference from related intracellular metabolites.
- Streamlined One-Step Protocol: Simplified procedure reduces hands-on time and potential errors, accelerating your research workflow.
- Flexible Format Options: Choose between 48T (up to 42 samples) and 96T (up to 90 samples) configurations to match your throughput needs perfectly.
- Cost-Effective for High-Throughput: Our standard wholesale pricing for human ELISA kits starts under $100, making routine screening and large-scale studies financially sustainable.
- Comprehensive Support: Leverage our custom ELISA development service for unique project requirements or utilize our free sample testing service to validate assay performance with your specific samples before purchase.
Assay Principle: One-Step Sandwich ELISA
This kit employs a sensitive one-step sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of human IP3:
- Capture: A monoclonal antibody specific for Inositol 1,4,5-Trisphosphate (IP3) is pre-coated onto the microplate wells.
- Simultaneous Binding: Standards or samples are added to the wells simultaneously with a Horseradish Peroxidase (HRP)-conjugated detection antibody. This allows the target IP3 to be “sandwiched” between the immobilized antibody and the enzyme-linked antibody in a single incubation step.
- Signal Development: After a wash to remove unbound conjugates, Tetramethylbenzidine (TMB) substrate is added. HRP catalyzes TMB’s conversion to a blue product.
- Quantification: The reaction is stopped with acid, turning the solution yellow. The absorbance (Optical Density, OD) at 450 nm is measured. The signal intensity is directly proportional to the IP3 concentration present in the original sample.
Kit Specifications & Components
Available Formats & Sample Capacity
| Format | Plate Layout | With Single Standard Curve (Samples) | With Duplicate Standard Curve (Samples) |
|---|---|---|---|
| 48T Kit | 12 wells × 4 strips | Up to 42 | Up to 36 |
| 96T Kit | 12 wells × 8 strips | Up to 90 | Up to 84 |
Standard Curve Concentration Points
S0-S5: 0, 30, 60, 120, 240, 480 pg/mL
Complete Kit Components
| Component | 96T Configuration | 48T Configuration |
|---|---|---|
| Pre-coated Microplate | 12 well × 8 strips | 12 well × 4 strips |
| Standard (S0-S5) | 0.3mL × 6 tubes | 0.3mL × 6 tubes |
| Sample Diluent | 6 mL | 3 mL |
| Detection Antibody-HRP | 10 mL | 5 mL |
| 20× Wash Buffer | 25 mL | 15 mL |
| Substrate A | 6 mL | 3 mL |
| Substrate B | 6 mL | 3 mL |
| Stop Solution | 6 mL | 3 mL |
| Plate Sealer | 2 pcs | 2 pcs |
| User Manual | 1 copy | 1 copy |
| Zipper Bag | 1 pc | 1 pc |
Note: The 20× Wash Buffer must be diluted with distilled water as per the manual before use.
Primary Research Applications
- Cell Signaling & GPCR Research: Investigate receptor-activated phospholipase C pathways and downstream signaling events.
- Calcium Signaling Studies: Correlate IP3 generation with intracellular calcium flux and release from endoplasmic reticulum stores.
- Neuroscience: Study the role of IP3 in synaptic plasticity, neuronal communication, and related disorders.
- Drug Discovery: Screen and evaluate compounds targeting GPCRs, PLC enzymes, or IP3 receptors.
Frequently Asked Questions (FAQ)
Q: What sample types require special preparation for this kit?
A: This kit is designed for cell or tissue lysates. Proper lysis buffer (often containing LiCl to inhibit IP3 phosphatases) is critical. Centrifugation is required to obtain clear supernatants for assay. Refer to the manual for detailed protocols.
Q: Why is your pricing for human ELISA kits so competitive?
A: Our optimized production and direct-to-researcher model allow us to offer high-performance reagents without the typical markups, ensuring consistent value with bulk pricing under $100.
Q: How does the one-step format benefit my research?
A: It consolidates incubation steps, significantly reducing total assay time and minimizing procedural complexity, which increases throughput and reduces potential pipetting errors.
Q: Is technical support available?
A: Absolutely. We provide comprehensive product manuals and dedicated email/phone support for assay setup, troubleshooting, and data interpretation.
Why Yanda Bio is Your Partner in Signaling Research
Yanda Bio is committed to advancing life science research with reliable, accessible tools. Our Human IP3 ELISA Kit exemplifies this mission, providing a robust, user-friendly, and economically sensible solution for quantifying a pivotal second messenger. With flexible formats, supportive services, and our commitment to affordability, we ensure you have the right resources to uncover new insights in cell signaling pathways.
Propel your research forward with precision and value. Inquire now to order or learn more about our services.
