Human OPRM1 ELISA Kit Materials supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0,50, 100,200,400,800 μg/ml
Key Advantages & Features
- Ultra-High Sensitivity: Detect OPRM1 levels as low as < 1.0 pg/mL, enabling the study of low-abundance targets in complex samples.
- Wide & Precise Detection Range: Achieve accurate quantification across a broad 1.0 pg/mL to 400 pg/mL dynamic range with excellent linearity.
- Exceptional Specificity: Developed with highly specific antibodies to ensure minimal cross-reactivity with other soluble structurally similar molecules.
- Proven Reproducibility: Low intra- and inter-assay coefficients of variation (<15%) guarantee consistent and reliable results across plates and batches.
- Unbeatable Value: Obtain high-quality data without the high cost through our competitive bulk pricing (under $100 per kit).
- Flexible Service Options: Beyond purchasing the kit, we offer a comprehensive custom ELISA development service and a complimentary sample testing option to validate your findings.
Assay Principle: One-Step Sandwich ELISA
This kit utilizes a sensitive and efficient one-step sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of Human OPRM1:
- Capture: Microplate wells are pre-coated with an antibody specific to the Mu-type Opioid Receptor (OPRM1).
- Simultaneous Binding: Samples, standards, and the HRP-conjugated detection antibody are added simultaneously to the wells. The target OPRM1 is “sandwiched” between the immobilized capture antibody and the enzyme-linked detection antibody in a single incubation step.
- Signal Development: After washing to remove unbound substances, a TMB substrate solution is added. The horseradish peroxidase (HRP) catalyzes the conversion of TMB into a blue-colored product.
- Measurement & Analysis: The reaction is stopped with an acid solution, turning the final product yellow. The absorbance (Optical Density, OD) is measured at 450 nm. The intensity of the color is directly proportional to the concentration of OPRM1 present in the original sample.
Detailed Kit Performance Specifications
| Parameter | Specification |
|---|---|
| Accuracy (Linearity) | Correlation coefficient R value ≥ 0.9900 |
| Sensitivity | Minimum detectable concentration < 1.0 pg/mL |
| Assay Range | 1.0 pg/mL – 400 pg/mL |
| Specificity | No significant cross-reactivity with analogous proteins |
| Precision (Repeatability) | Intra-assay CV < 10%; Inter-assay CV < 15% |
| Storage Conditions | 2-8°C, protected from light and moisture |
| Shelf Life | 6 months from date of manufacture |
Primary Research Applications
- Neuroscience & Pain Research: Investigate receptor expression and regulation in pain pathways and analgesic drug mechanisms.
- Addiction & Substance Use Studies: Explore OPRM1’s role in reward circuitry, dependency, and behavioral responses.
- Psychiatric Disorder Research: Study potential alterations in receptor density linked to mood and affective disorders.
- Drug Discovery & Development: Screen and evaluate novel opioid agonists, antagonists, or modulators in pre-clinical models.
Ordering & Service Options
Yanda Bio provides flexible solutions to meet your exact research needs.
Option 1: Purchase the Standard Kit
- Price: Starting from $99 for a standard 96-well kit.
- Ideal for: Labs with established protocols requiring a reliable, off-the-shelf solution.
Option 2: Custom ELISA Kit Development
- Service: Tailor the kit’s sensitivity, detection range, or sample compatibility to your unique project requirements.
- Ideal for: Novel targets, specific sample matrices, or non-standard assay conditions.
Option 3: Free Sample Testing Service
- Service: Send us your samples. Our experts will run the assay using this kit and provide you with the detailed data report.
- Ideal for: Preliminary feasibility studies or labs without immediate access to ELISA instrumentation.
Contact our technical sales team for a bulk quote, custom project consultation, or to arrange your complimentary sample testing.
Frequently Asked Questions (FAQ)
Q: What sample types are compatible with this kit?
A: The kit is optimized for tissue homogenates and cell lysates. For other sample types (e.g., serum), please inquire about our customization service.
Q: How does your pricing remain below $100?
A: Our optimized manufacturing processes and direct-to-researcher model allow us to offer high-performance kits at an accessible price point without compromising on quality or validation.
Q: What is the “free testing service”?
A: It’s a risk-free way to evaluate the kit’s performance with your specific samples. You provide the samples, and we perform the assay at our facility, delivering a full data report.
Q: Is this kit suitable for measuring membrane-bound receptors?
A: Yes, this kit is designed to detect OPRM1 in lysates where membrane proteins have been solubilized. Proper sample preparation is key.
Why Choose Yanda Bio for Your Receptor Research?
Yanda Bio is your dedicated partner in life science research. Our Human Mu-type Opioid Receptor (OPRM1) ELISA Kit embodies our commitment to scientific rigor, operational simplicity, and outstanding value. With industry-leading sensitivity, transparent performance specifications, and unmatched support through customization and testing services, we provide more than just a product—we provide a solution tailored to your success.
Advance your neuropharmacology research today. Order your kits or contact us to discuss your custom needs.
