Select Your Optimal Assay Format
We offer two optimized kit tiers to suit your specific sensitivity requirements and budget. Purchase any tier and receive our complimentary sample testing service.
| Feature | Tier 1: Standard MHPG ELISA Kit | Tier 2: High-Sensitivity MHPG ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with proprietary matrix dilution. | Biotinylated recombinant mAb (>95% pure) + Streptavidin-HRP (≥500 U/mg). |
| Key Advantage | Cost-Effective & Robust. Utilizes a simultaneous incubation step and a specialized diluent that minimizes background interference from compounds like hemoglobin and lipids, ensuring stable assay performance. | Ultimate Sensitivity & Specificity. The biotin-streptavidin amplification system effectively bypasses matrix effects, delivering superior signal-to-noise for detecting low-abundance analytes in complex samples. |
| Ideal For | Routine analysis of urine or plasma where MHPG concentrations are typically higher. | Demanding applications requiring maximum sensitivity, such as measuring MHPG in CSF or low-volume tissue extracts. |
Complimentary Expert Testing Service: Fast, Reliable Data
Leverage our professional laboratory expertise to save time and ensure optimal results. Our service is included with your kit purchase.
- Rapid Execution: We begin your assay within 24 hours of receiving your samples, with a typical turnaround of 5 business days for the complete report.
- Comprehensive Data Delivery: You receive a full data package, including raw optical density (OD) values, calculated concentration tables, and a standard curve graph.
- Risk-Free Validation: This service is ideal for confirming assay performance with your specific sample matrices.
Our Uncompromising Commitment to Research Integrity
We build trust through actionable commitments that support your scientific journey:
- Publication Reward: Cite this MHPG ELISA Kit in your research paper with a link to our product page, and we will send you a complimentary standard ELISA kit (up to $120 value) in recognition of your work.
- 100% Satisfaction Guarantee: We stand behind our products. If a kit does not perform as specified, we offer an unconditional replacement or refund.
- Dedicated Support: Our technical and customer service teams commit to providing a proposed solution within 24 hours of any inquiry.
- Guardians of Your Science: We handle every sample with the utmost respect and precision, understanding its invaluable role in your research.
Assay Principle & Technical Specifications
Principle: The kit employs a one-step sandwich ELISA. MHPG in the sample is captured between a pre-coated antibody and an HRP-conjugated detection antibody in a single incubation. The subsequent TMB reaction, measured at 450 nm, yields a signal proportional to MHPG concentration.
Performance Specifications:
- Sensitivity: < 1.0 ng/mL.
- Detection Range: 1.0 – 200 ng/mL.
- Accuracy (Linearity): Correlation Coefficient R ≥ 0.9900.
- Precision: Intra- & Inter-assay Coefficient of Variation (CV) < 15%.
- Specificity: Minimal cross-reactivity with structurally similar metabolites.
- Storage: 2-8°C; stable for 6 months.
Sample Preparation: Urine and plasma samples often require specific pre-treatment or dilution as per protocol. All samples should be clarified by centrifugation (3000×g for 10-30 mins) and stored at -20°C in single-use aliquots to avoid freeze-thaw degradation.
Streamlined Assay Workflow
- Addition: Add 50 µL of standards or prepared samples to the antibody-coated wells.
- Incubation: Add 100 µL of HRP-conjugated Detection Antibody. Incubate at 37°C for 60 minutes.
- Wash: Aspirate and wash the plate 5 times thoroughly with the provided wash buffer.
- Development: Add 50 µL each of Substrate A and B. Incubate at 37°C for 15 minutes protected from light.
- Measurement: Add 50 µL Stop Solution. Read the absorbance at 450 nm within 15 minutes.
Pricing & Service Options
| Option | Description | Lead Time / Price |
|---|---|---|
| Tier 1: Standard Kit Purchase | Reliable, cost-effective solution for in-lab use. | Retail: $120. Bulk: < $100 (quote required). |
| Tier 2: High-Sensitivity Kit Purchase | Premium performance for maximum sensitivity needs. | Competitive manufacturer price available upon request. |
| Expert Testing Service | We run the assay and provide a complete data report. | ~5 business days. Free with kit purchase. |
| Custom ELISA Development | Tailored solutions for unique project requirements. | Project-based quotation. |
To order your MHPG ELISA Kit or inquire about our services, contact Yanda Bio, your trusted direct manufacturer in China.
Applications & Frequently Asked Questions (FAQ)
Primary Research Applications (RUO): Neuropsychiatric disorder research (depression, anxiety, PTSD), neurodegenerative disease studies, stress physiology, psychopharmacology (drug mechanism studies), and biomarker discovery.
Q: What sample type is most commonly used with this kit?
A: Urine is frequently used for MHPG measurement as it reflects systemic norepinephrine turnover. Plasma and CSF are also applicable for more specific central or peripheral activity assessments.
Q: How do I claim the free kit for publication?
A: Once your peer-reviewed article citing the “3-methoxy-4-hydroxyphenyl ethylene glycol (MHPG) ELISA Kit (Yanda Bio)” with a link to our website is published, send us the citation. We will promptly ship your reward.
Q: Why are there two kit tiers?
A: To provide flexibility. Tier 1 offers excellent value for standard applications. Tier 2, with its advanced amplification, is for projects requiring the lowest possible detection limits or working with the most challenging sample matrices.
Q: How can I buy this ELISA kit from China?
A: As the direct source manufacturer in China, you can buy this MHPG ELISA Kit online by contacting us directly. We handle global shipping and provide all necessary export documentation.
Manufacturer’s Note
This product is for Research Use Only (RUO). It is not intended for diagnostic, therapeutic, or clinical procedures. Researchers are responsible for determining the kit’s suitability for their specific experimental models.
