Choose Your Method: Sandwich vs. Competitive ELISA
To accommodate diverse sample types and research objectives, we offer the Mouse 2-AG ELISA Kit in two distinct methodological formats. Purchasing either format includes our complimentary expert testing service.
Format Comparison
| Feature | Sandwich ELISA Format | Competitive ELISA Format |
|---|---|---|
| Detection Principle | Direct “sandwich” capture of the 2-AG molecule between two antibodies. | Competition between sample 2-AG and an HRP-labeled 2-AG analog for limited antibody binding sites. |
| Signal Relationship | Signal intensity is positively correlated with 2-AG concentration. | Signal intensity is inversely correlated with 2-AG concentration (higher sample 2-AG = lower OD). |
| Typical Advantages | Often provides higher specificity and a wider dynamic range for complex matrices. | Excellent for detecting small molecules like 2-AG, often offering superior sensitivity in biological fluids. |
| Best Application | Ideal for samples where 2-AG is expected to be present in moderate to high abundance, or when maximum specificity is required. | Recommended for achieving the highest sensitivity (<0.1 nmol/L), especially in plasma/serum or for detecting subtle physiological changes. |
Our Technical Tip: For first-time users or precious samples, the Competitive Format is frequently recommended for 2-AG due to its optimized sensitivity for small lipid mediators in biological fluids.
Select Your Performance Tier: Standard or High-Sensitivity
Within your chosen method (Sandwich or Competitive), select the optimal performance tier for your needs. Both tiers are available in either format.
| Feature | Tier 1: Standard 2-AG ELISA Kit | Tier 2: High-Sensitivity 2-AG ELISA Kit |
|---|---|---|
| Core Technology | Optimized protocol with a proprietary, interference-reducing diluent. | Biotinylated antibody (>95% pure) + Streptavidin-HRP conjugate (≥500 U/mg). |
| Key Advantage | Proven Reliability & Excellent Value (>99% success rate). Reduces background interference from hemoglobin and lipids for stable results. | Maximum Sensitivity & Matrix Immunity. Signal amplification directly neutralizes matrix effects for low-abundance targets or challenging samples. |
| Price Advantage | Retail: $120. Bulk: <$100. A low-cost, high-quality alternative offering savings versus premium brands. | Significantly more cost-effective than comparable high-sensitivity kits from leading international suppliers. |
| Selection Advice | Ideal for routine research with common sample types. | Recommended for precious/rare samples (e.g., micro-dissected brain regions) or detecting very low concentrations. |
Unbeatable Value & Direct Savings
Why pay premium prices? Our efficient in-house production and direct sales model allow us to offer the Mouse 2-AG ELISA Kit at a fraction of the cost of leading international brands, without sacrificing the rigorous validation and performance your research demands.
Complimentary Expert Testing Service
Validate your assay or save time with our professional service, included with every kit purchase.
- Rapid Processing: Analysis begins within 24 hours of sample receipt.
- Complete Reporting: Receive a full data package in ~5 business days, including raw OD values, concentration tables, and a standard curve.
Our Commitment to Your Research
- Publication Reward: Cite this kit with a link to our site, and receive a free standard ELISA kit.
- 100% Satisfaction Guarantee: Unconditional replacement or refund if specifications are not met.
- 24-Hour Support: A proposed solution within one business day.
- Custom Service: Available through our ELISA kit custom service.
Assay Specifications
Detection Range: 0.1 – 40 nmol/L
Sensitivity: < 0.1 nmol/L
Accuracy: R ≥ 0.9900
Precision: Intra- & Inter-assay CV < 15%
Specificity: High specificity with minimal cross-reactivity.
Storage: 2-8°C; 6-month shelf life.
Sample Preparation: For brain tissue, homogenize in appropriate ice-cold extraction solvent (e.g., acetonitrile-containing) to preserve 2-AG. For plasma/serum, rapid processing and centrifugation at 3000×g is critical. Aliquot and store at -80°C, avoiding any freeze-thaw cycles. Specific extraction protocols are provided in the detailed manual.
Streamlined Workflow (General)
- Add standards or pre-extracted samples to pre-coated wells.
- Add Detection Reagent (HRP-antibody or HRP-competitor).
- Incubate at 37°C for 60 min.
- Wash plate 5 times.
- Add TMB Substrate. Incubate at 37°C for 15 min (dark).
- Add Stop Solution. Read absorbance at 450 nm.
Calculation: Generate a standard curve to determine sample concentrations.
Ordering & Custom Services
| Option | Description | Details |
|---|---|---|
| Tier 1: Standard Kit | Low-cost, reliable solution. (Specify Sandwich/Competitive) | Retail: $120. Bulk: <$100 (quote). |
| Tier 2: High-Sensitivity Kit | Cost-effective premium performance. (Specify Sandwich/Competitive) | Contact for price. |
| Expert Testing Service | We run the assay for you. | Free with purchase. |
| Custom ELISA Development | Tailored solutions. | Project-based quote. |
To buy the Mouse 2-AG ELISA Kit, contact Yanda Bio for pricing and to discuss the best format for your research.
Research Applications & FAQ
Primary Applications (RUO): Neuroscience (synaptic plasticity, pain, fear memory), immunology (neuroinflammation), metabolic disease research, and studying the effects of cannabinoids or cannabinoid receptor modulation.
Q: What is the main function of 2-AG?
A: 2-AG is the most abundant endocannabinoid and a full agonist of the CB1 and CB2 receptors. It acts as a key retrograde messenger in the central nervous system, modulating neurotransmitter release, and plays roles in peripheral immune and metabolic regulation.
Q: Should I choose the Sandwich or Competitive format?
A: For most applications measuring 2-AG in biological fluids (plasma, brain homogenate), the Competitive ELISA format is typically recommended due to its superior sensitivity for this small lipid molecule. Contact our support for guidance on your specific project.
Q: How do your prices compare to other brands?
A: Through direct manufacturing (Mouse 2-AG ELISA Kit production), we offer a truly cost-effective alternative, providing significant savings compared to kits from leading international suppliers while maintaining high performance standards.
Q: How do I claim the free publication kit?
A: Send us the citation (DOI) of your article citing “Mouse 2-AG ELISA Kit (Yanda Bio)” with a link to our product page.
Manufacturer’s Note
For Research Use Only (RUO). Not for diagnostic use. Proper sample extraction is critical for accurate 2-AG measurement.
