Mouse 3-Indolelactic Acid (ILA) ELISA Kit supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0, 10,20,40,80, 160 pg/ml
Target Introduction: 3-Indolelactic Acid (ILA)
3-Indolelactic Acid (ILA) is a key microbial metabolite derived from tryptophan, playing a significant role in the gut-brain axis, immune modulation, and metabolic homeostasis. Precise measurement of ILA is essential for research in microbiome studies, inflammatory diseases, neuroimmunology, and metabolic disorders.
Yanda Bio’s Mouse 3-Indolelactic Acid (ILA) ELISA Kit is engineered to deliver accurate and consistent results for this important biomarker. As a dedicated manufacturer with an extensive portfolio of over 6,000 detectable targets and established collaborations with more than 60 universities, we specialize in providing robust, cost-effective Mouse ELISA Kit solutions to accelerate scientific discovery.
Superior Value: Premium Components, Direct Pricing
A critical factor in ELISA performance is the quality of the core reagents—specifically, the antibodies and the solid phase. High-priced kits often excel due to high-activity antibodies and superior plate binding capacity.
Our Quality Promise: We utilize high-affinity antibodies sourced from leading US suppliers, matching the quality of premium brands. Furthermore, we employ high-binding-capacity microplates to ensure optimal adsorption of capture antibodies, maximizing assay consistency. Our optimized ELISA Kit production process integrates these premium materials into a streamlined, low-cost ELISA Kit model, delivering exceptional performance without the premium price tag.
Two Optimized Formats for Every Research Need
To accommodate diverse experimental requirements, we offer this assay in two specialized configurations.
Format 1: Standard Performance Kit
Designed for reliable, high-value routine analysis. This kit features a simplified one-step incubation, enabling simultaneous antigen binding. A proprietary diluent effectively minimizes interference from common sample matrix components (e.g., hemoglobin, lipids), ensuring low background and high reproducibility. With a proven track record of >99% success rate in validated applications, it represents our most accessible pricing tier.
- Typical Price: Approximately $120 (retail). Under $100 per kit for wholesale or bulk orders.
Format 2: High-Sensitivity Kit
Developed for demanding applications involving low-abundance targets or scarce, precious samples. This format employs a biotinylated recombinant monoclonal antibody (purity >95%) paired with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This advanced system provides significant signal amplification, effectively mitigates matrix effects, and is designed to ensure success in critical, one-attempt experiments.
Choosing the Right Format:
- Select Format 1 for cost-effective, reliable quantification of ILA in standard research settings with sufficient sample volume.
- Select Format 2 when studying trace levels, working with limited or irreplaceable samples, or when maximum detection sensitivity is paramount.
Assay Principle
This kit is based on a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) principle. The microplate is pre-coated with an antibody specific to Mouse ILA. Standards and samples are pipetted into the wells, and any ILA present is bound by the immobilized antibody. After washing, an HRP-conjugated detection antibody is added. Following another wash step, TMB substrate is added. The enzyme-catalyzed reaction produces a blue color that turns yellow upon addition of the stop solution. The optical density (OD) measured at 450nm is proportional to the concentration of ILA in the sample.
Key Benefits & Support
- Uncompromised Quality, Direct Savings: Receive kits built with premium imported antibodies on high-binding plates via our efficient cost-effective Mouse ELISA Kit model.
- Complimentary Testing Service: Purchase any Yanda Bio ELISA kit and receive FREE expert testing—provide your samples, and we return precise data.
- Publication Incentive: Cite our product and link to our website in your manuscript to receive a free standard ELISA kit.
- 100% Satisfaction Guarantee: We stand by our承诺. If product performance does not meet specifications, we offer a prompt replacement or full refund.
- Dedicated 24-Hour Support: Access our technical team and receive a proposed solution within one business day.
- Custom Assay Development: Leverage our expertise with comprehensive custom ELISA kit services for specialized research needs.
Detailed Specifications
- Accuracy: Standard curve linear regression correlation coefficient R ≥ 0.9900.
- Precision: Intra-assay CV < 10%; Inter-assay CV < 15%.
- Specificity: No significant cross-reactivity with analogous soluble structures.
- Sample Compatibility: Validated for serum, plasma (EDTA, citrate, heparin), cell culture supernatant, and tissue homogenate.
- Stability & Storage: Store complete kit at 2-8°C. Stable for 6 months.
