The Mouse 5-HT ELISA Kit consists of the following components
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0,30,60, 120,240,480 ng/mL
Value Without Compromise: Choose Your Precision Tier
Our Mouse 5-Hydroxytryptamine (5-HT) assay is available in two optimized formats to suit your sensitivity requirements and budget. Purchasing either tier includes our complimentary expert sample testing service.
| Feature | Tier 1: Standard Mouse 5-HT ELISA Kit | Tier 2: High-Sensitivity Mouse 5-HT ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with a proprietary matrix-suppression diluent. | Biotinylated recombinant monoclonal antibody (>95% pure) + Streptavidin-HRP conjugate (≥500 U/mg). |
| Key Advantage | Exceptional Value & Proven Reliability (>99% success rate). Employs a simultaneous incubation for efficiency. The specialized diluent minimizes background from hemoglobin and lipids, ensuring robust performance. | Ultimate Sensitivity & Matrix Immunity. The advanced biotin-streptavidin system directly neutralizes matrix effects, delivering superior signal-to-noise for the most demanding applications. |
| Price Advantage | Retail: $120. Bulk: <$100. A low-cost, high-quality alternative offering savings compared to premium brands. | Significantly more cost-effective than comparable high-sensitivity kits from leading suppliers, without sacrificing performance. |
| Our Selection Advice | Ideal for routine neuroscience & behavioral research. | Recommended for precious samples (e.g., micro-dissected brain regions) or detecting subtle physiological changes. |
Unbeatable Value Proposition
Why pay premium prices? Yanda Bio’s Mouse 5-Hydroxytryptamine (5-HT) kits are part of our commitment to providing cost-effective, high-performance research tools through efficient in-house production. Our direct-to-researcher model eliminates distributor markups, allowing us to offer:
- Superior Pricing: Obtain reliable data at a low-cost point, with bulk options under $100.
- Comparable Performance: Our kits are rigorously validated to deliver sensitivity and specificity on par with leading international brands.
- Included Value: Enjoy free expert testing services and comprehensive support often offered as expensive add-ons elsewhere.
Complimentary Expert Testing Service
Maximize efficiency and ensure results with our professional service, included with every purchase.
- Rapid Processing: Analysis begins within 24 hours of sample receipt.
- Complete Reporting: Receive a full data package in ~5 business days, including raw OD values, concentration tables, and a standard curve.
Our Commitment to Your Research
- Publication Reward: Cite this kit with a link to our site in your publication, and receive a free standard ELISA kit.
- 100% Satisfaction Guarantee: We promise performance with an unconditional replacement or refund.
- 24-Hour Support: Get a proposed solution within one business day.
Assay Principle & Specifications
Detection Principle: This sandwich ELISA captures mouse 5-HT between an immobilized antibody and an HRP-conjugated detection antibody. The TMB substrate reaction, measured at 450 nm, yields a signal proportional to serotonin concentration.
Performance Specifications:
- Sensitivity: < 1.0 ng/mL.
- Detection Range: 1.0 – 480 ng/mL.
- Accuracy: R ≥ 0.9900.
- Precision: Intra- & Inter-assay CV < 15%.
- Specificity: High specificity with minimal cross-reactivity.
- Storage: 2-8°C; 6-month shelf life.
Sample Preparation: For brain or intestinal tissue, use appropriate homogenization buffers. For serum/plasma, centrifuge at 3000×g for 10-30 mins. Aliquot and store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. A 1:5 dilution is typically required for liquid samples.
Streamlined Assay Protocol
- Add 50 µL of standards or prepared samples to the pre-coated wells.
- Add 100 µL HRP-conjugated Detection Antibody (except blank). Incubate at 37°C for 60 min.
- Wash the microplate 5 times thoroughly.
- Add 50 µL each of Substrate A & B. Incubate at 37°C for 15 min (dark).
- Add 50 µL Stop Solution. Read absorbance at 450 nm within 15 min.
Ordering & Custom Services
| Option | Description | Details |
|---|---|---|
| Tier 1: Standard Kit | Low-cost, reliable solution for common research. | Retail: $120. Bulk: <$100 (quote). |
| Tier 2: High-Sensitivity Kit | Cost-effective premium performance. | Contact for manufacturer price. |
| Expert Testing Service | We run the assay for you. | Free with kit purchase. |
| Custom ELISA Development | Tailored solutions. | Project-based quote. |
Ready to buy a high-quality, low-cost Mouse 5-HT ELISA Kit? Contact Yanda Bio for pricing or a custom quote.
Research Applications & FAQ
Primary Applications (RUO): Depression & anxiety research, neurodegenerative disease studies (e.g., Alzheimer’s), gastrointestinal physiology (IBS research), sleep/wake cycle studies, and psychopharmacology (drug mechanism of action).
Q: Why is serotonin (5-HT) an important research target?
A: 5-HT is a key neurotransmitter regulating mood, cognition, and numerous peripheral functions. Its dysregulation is implicated in major depression, anxiety disorders, migraines, and GI disorders, making it a central biomarker in biomedical research.
Q: How do your prices compare to other major brands?
A: Through efficient in-house production, we offer Mouse 5-Hydroxytryptamine (5-HT) kits that are significantly more cost-effective than comparable products from leading international suppliers, providing exceptional value without compromising data quality.
Q: Can this kit be used for rat samples?
A: It is validated for mouse samples. For rat samples, please contact us about our custom service for cross-reactivity validation or species-specific development.
Q: How do I claim the free publication kit?
A: Send us the citation (DOI) of your article citing “Mouse 5-Hydroxytryptamine (5-HT) ELISA Kit (Yanda Bio)” with a link to our product page.
Manufacturer’s Note
For Research Use Only (RUO). Not for diagnostic use.
