Mouse 6-OHDA ELISA Kit Materials supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0 、5 、10 、20 、40 、80ng/mL.
Target Overview: 6-Hydroxydopamine (6-OHDA)
6-Hydroxydopamine (6-OHDA) is a selective neurotoxin widely used in preclinical research to create experimental models of Parkinson’s disease by inducing the degeneration of dopaminergic and noradrenergic neurons. Accurate quantification of 6-OHDA levels is critical for studies involving neurotoxicity mechanisms, neuroprotection screening, and validating model efficacy in neuroscience research.
Yanda Bio’s Mouse 6-Hydroxydopamine (6-OHDA) ELISA Kit provides a sensitive, specific, and reliable tool for these demanding applications. As a specialized manufacturer with a portfolio of over 6,000 detectable targets and collaborative partnerships with more than 60 domestic universities, we are dedicated to supporting groundbreaking research with high-performance, accessible solutions. Discover the advantages of our streamlined ELISA Kit production process.
Premium Performance at an Accessible Price Point
We understand that research budgets are paramount. A common question is: how can Yanda Bio offer such competitive pricing without sacrificing quality? The answer lies at the heart of the assay—the antibody.
Our Quality Commitment: The performance of an ELISA kit is fundamentally determined by the activity and specificity of its antibodies. Our kits utilize high-affinity antibodies sourced from leading US suppliers, ensuring core reagent quality on par with premium international brands. By optimizing our manufacturing and supply chain, we deliver this premium performance within a highly cost-effective Mouse ELISA Kit framework, offering exceptional value for your neuroscience research.
Choose the Optimal Format for Your Experiment
We offer two meticulously designed kit formats to suit specific experimental priorities, from cost-efficiency to maximum sensitivity.
Format A: High-Value Standard Kit
Ideal for routine quantification where sample availability is not limiting. This kit employs an efficient one-step incubation procedure, allowing simultaneous binding of the antigen to both the immobilized and enzyme-conjugated antibodies. Its specialized sample diluent minimizes background interference from common biomatrix components like hemoglobin and lipids, ensuring stable and reproducible results. Proven through extensive validation with a success rate exceeding 99%, this is our flagship low-cost ELISA Kit option.
- Typical Pricing: Around $120 (retail). Less than $100 per unit for wholesale or volume purchases.
Format B: Advanced Sensitivity Kit
Engineered for the most challenging scenarios involving trace-level detection or irreplaceable samples. This format features a biotinylated recombinant monoclonal antibody (purity >95%) combined with a high-activity Streptavidin-HRP complex (≥500 U/mg). This powerful signal amplification system dramatically enhances sensitivity, effectively circumvents matrix effects, and is designed to guarantee success in critical, one-attempt experiments.
Selection Guide:
- Select Format A for reliable, budget-conscious measurement in well-established models or with abundant samples.
- Select Format B for pioneering research on low-concentration analytes, when working with miniscule or precious sample volumes, or when ultimate detection power is non-negotiable.
Assay Methodology
This kit operates on a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) principle. Microplate wells are pre-coated with an antibody specific to Mouse 6-OHDA. Samples and standards are added, followed by the addition of an HRP-conjugated detection antibody to form an antibody-antigen-antibody “sandwich” complex. After incubation and washing, a TMB substrate is added. The catalytic reaction yields a blue product that turns yellow upon acidification. The intensity of the yellow color, measured at 450nm, is directly proportional to the concentration of 6-OHDA present in the original sample.
Key Benefits & Customer Support
- Professional Quality, Direct Savings: Access superior kits built with imported, high-grade antibodies through our efficient cost-effective Mouse ELISA Kit model.
- Free Testing Service: Purchase any Yanda Bio ELISA kit and qualify for complimentary testing by our experts—submit your samples, and we deliver the data.
- Publication Recognition: Acknowledge our kit and link to our product page in your peer-reviewed article to receive a free standard ELISA kit as our token of appreciation.
- Unconditional Guarantee: We uphold our承诺 with confidence. Should performance not meet published specifications, we offer an immediate replacement or full refund.
- 24-Hour Technical Support: Connect with our specialist team and receive a targeted solution proposal within one business day.
- Custom Assay Development: Inquire about our end-to-end custom ELISA kit services for unique or novel research targets.
Detailed Specifications
- Accuracy: Standard curve linear regression demonstrates a correlation coefficient R of ≥ 0.9900.
- Precision: Intra-assay Coefficient of Variation (CV) < 10%; Inter-assay CV < 15%.
- Specificity: Exhibits no significant cross-reactivity with other relevant catecholamine analogs.
- Sample Types: Fully validated for serum, plasma (EDTA, citrate, heparin), cell culture supernatant, and tissue homogenate.
- Stability & Storage: The complete kit is stable for 6 months when stored properly at 2-8°C.
