Mouse Ferredoxin 1 (FDX1) / Adrenodoxin ELISA Kit for Metabolic & Disease Research
Yanda Bio’s Mouse FDX1 (Adrenodoxin) ELISA Kit provides a highly sensitive and specific tool for the quantitative measurement of Ferredoxin 1 in mouse serum, plasma, tissue lysates (especially liver and adrenal gland), and cell culture supernatants. FDX1 is a critical iron-sulfur protein that transfers electrons within mitochondria, playing an indispensable role in steroid hormone synthesis, vitamin D metabolism, bile acid production, and cellular iron homeostasis. This kit is essential for research involving endocrine disorders, mitochondrial function, metabolic diseases, and drug-induced toxicity studies in mouse models.
As a certified factory-direct manufacturer with state-of-the-art laboratories in China, we combine premium quality with exceptional value. This ELISA kit made in China is available at a retail price of $120, with bulk pricing under $100. Our capabilities are underscored by a portfolio of over 6,000 detection targets across key research areas and sustained collaborations with 70+ universities globally.
Two Performance Tiers for Precision Research
To address diverse experimental needs, we offer two optimized kit configurations. Purchasing either tier includes our complimentary expert sample testing service.
| Feature | Tier 1: Standard Sensitivity Mouse FDX1 ELISA Kit | Tier 2: High-Sensitivity Mouse FDX1 ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with a proprietary background-suppression diluent. | Biotinylated recombinant monoclonal antibody (>95% pure) paired with Streptavidin-HRP conjugate (≥500 U/mg). |
| Key Advantage | Excellent Value & Reliability. Features a single-step incubation and a specialized diluent that effectively minimizes interference from hemoglobin and lipids, ensuring stable and consistent assay performance in complex biological matrices. | Ultimate Sensitivity & Specificity. The advanced biotin-streptavidin signal amplification system directly circumvents matrix effects, delivering an unparalleled signal-to-noise ratio for detecting ultra-low abundance FDX1 in precious or challenging samples. |
| Best For | Routine monitoring of FDX1 expression in standard mouse models where sample material is not limiting. | High-stakes research requiring the lowest detection limits (<0.1 pg/mL), analysis of limited sample volumes, or work with samples prone to high background. |
Included Expert Testing Service: Fast, Reliable Data Without the Hassle
Maximize your research efficiency by leveraging our professional laboratory services, included with your purchase.
- Guaranteed Rapid Start: We initiate your analysis within 24 hours of sample receipt.
- Fast, Comprehensive Reporting: Receive a full data package in as little as 5 business days, containing raw OD values, calculated concentration tables, and a standard curve graph.
- Effortless Validation: The perfect solution to confirm assay performance with your unique sample types before committing to large-scale in-house studies.
Our Unwavering Commitment to Your Research Integrity
We build long-term partnerships through concrete, actionable promises:
- Publication Recognition: Cite this Mouse FDX1 ELISA Kit and link to our product page in your publication, and we will gratefully send you a free standard ELISA kit (up to $120 value).
- 100% Performance Guarantee: We stand firmly behind our products. If any kit fails to meet its published specifications, we offer an unconditional replacement or refund.
- Responsive Support Commitment: Our dedicated team pledges to provide a clear path to resolution within 24 hours of any technical or service inquiry.
- Guardians of Scientific Integrity: We treat every sample with the utmost respect and precision, applying rigorous protocols to safeguard the validity of your research.
Technical Specifications & Assay Principle
Detection Principle: This kit employs a validated one-step sandwich ELISA. Mouse FDX1 in the sample is captured between an immobilized antibody and an HRP-conjugated detection antibody during a single incubation. The subsequent enzymatic reaction with TMB substrate produces a colorimetric signal measured at 450 nm, directly proportional to the FDX1 concentration.
Performance Characteristics:
- Sensitivity: < 0.1 pg/mL.
- Detection Range: 0.1 – 120 pg/mL.
- Accuracy (Linearity): Correlation Coefficient R ≥ 0.9900.
- Precision: Intra- and Inter-assay Coefficient of Variation (CV) < 15%.
- Specificity: High specificity for mouse FDX1, with minimal cross-reactivity.
- Storage & Stability: Store at 2-8°C, protected from light and moisture; stable for 6 months.
Sample Preparation: For tissue analysis, prepare homogeneous lysates in appropriate buffers. Clarify all samples (serum, plasma, lysates) by centrifugation (e.g., 3000×g for 10-15 minutes). Aliquot and store at -20°C, avoiding repeated freeze-thaw cycles.
Streamlined Assay Procedure
- Addition: Pipette 50 µL of standards or appropriately diluted samples into the antibody-coated wells.
- Incubation: Add 100 µL of the HRP-conjugated Detection Antibody. Incubate at 37°C for 60 minutes.
- Wash: Aspirate and wash the microplate 5 times thoroughly with the diluted wash buffer.
- Color Development: Add 50 µL each of Substrate A and B. Incubate at 37°C for 15 minutes, protected from light.
- Measurement: Add 50 µL Stop Solution. Measure the absorbance at 450 nm within 15 minutes.
Pricing & Service Options
| Option | Description | Details |
|---|---|---|
| Tier 1: Standard Kit | Cost-effective, reliable solution for in-laboratory use. | Retail: $120. Bulk: < $100 (quote required). |
| Tier 2: High-Sensitivity Kit | Premium performance for maximum sensitivity requirements. | Contact for competitive manufacturer price. |
| Expert Testing Service | Full-service assay execution and detailed data reporting. | ~5-day turnaround. Free with kit purchase. |
| Custom ELISA Development | Tailored assay solutions from our extensive portfolio of 6,000+ targets. | Project-based quotation available. |
To order your Mouse FDX1 ELISA Kit or to inquire about our vast assay catalog, contact Yanda Bio, your trusted direct manufacturer.
Research Applications & Frequently Asked Questions
Primary Applications (Research Use Only): Research on steroidogenesis and endocrine disorders, mitochondrial function and dysfunction studies, drug-induced hepatotoxicity, metabolic disease models (e.g., NAFLD, NASH), and investigations into cellular iron-sulfur cluster biogenesis.
Q: What is the significance of FDX1 in research?
A: FDX1 is a central protein in mitochondrial electron transfer. Its levels can indicate mitochondrial health and are crucial in pathways synthesizing steroids, heme, and bile acids, making it a key biomarker in metabolic and toxicological studies.
Q: How do I claim the free kit for a publication?
A: Upon publication of your peer-reviewed article that cites the “Mouse FDX1 (Adrenodoxin) ELISA Kit (Yanda Bio)” and includes a direct link to our product page, send us the citation details to claim your complimentary reward.
Q: Can this kit be used for rat samples?
A: This kit is specifically validated for mouse FDX1. While there may be cross-reactivity due to high homology, it is not guaranteed. For rat-specific quantification, please inquire about our custom ELISA development service.
Q: Why source from a direct manufacturer in China?
A: Partnering with Yanda Bio eliminates intermediaries, ensuring you receive the most competitive manufacturer price, direct access to our R&D team for support, and full transparency over our stringent quality control processes.
Manufacturer’s Note
This product is developed and sold for Research Use Only (RUO). It is not intended for diagnostic, therapeutic, or clinical procedures. The researcher assumes full responsibility for validating the kit’s suitability for their specific experimental system.
