Mouse Osteopontin (SPP1) ELISA Kit composition
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Mouse Osteopontin (SPP1) ELISA Kit for Immunology & Bone Biology Research
Yanda Bio’s Mouse Osteopontin (SPP1) ELISA Kit is designed for the specific and quantitative detection of Osteopontin (Secreted Phosphoprotein 1) in mouse biological sampeles, including serum, plasma, cell culture supernatants, and tissue homogenates. As a multifunctional glycoprotein, Osteopontin plays pivotal roles in inflammatory responses, immune cell recruitment, bone mineralization, and cancer progression. This reliable, ready-to-use kit provides researchers in immunology, osteology,
As a leading factory-direct manufacturer with professional laboratories in China, we ensure premium quality with exceptional value. This Mouse Osteopontin ELISA Kit made in China is available at competitive manufacturer-direct pricing, with significant savings for bulk orders under $100. It is part of our extensive portfolio of over 6,000 detection targets, supported by collaborative research with more than 70 universities globally.
Optimized Assay Design for Superior Performance
Our kit features an advanced one-step sandwich ELISA format engineered for simplicity and reliability. The protocol utilizes a simultaneous incubation step, where the sample antigen binds concurrently with the immobilized capture antibody and the enzyme-conjugated detection antibody. This streamlined approach reduces total assay time and potential pipetting errors.
A key component is our proprietary sample diluent, specifically formulated to minimize background interference from common sample matrix components such as hemoglobin and lipids. By reducing this background noise, the diluent ensures optimal antigen-antibody binding and delivers more stable, reproducible results with lower background variability.
Key Features & Benefits
- High Specificity & Sensitivity: Developed with antibodies highly specific to mouse Osteopontin (SPP1), enabling accurate quantification with a sensitivity of <0.1 ng/mL and minimal cross-reactivity.
- Streamlined One-Step Protocol: The efficient single-incubation design saves valuable hands-on time and accelerates your research workflow.
- Matrix Interference Reduction: The specialized diluent is core to ensuring assay robustness, particularly for complex samples like serum and plasma.
- Free Expert Testing Service: With every purchase, utilize our professional lab. We begin your assay within 24 hours of sample receipt and deliver a comprehensive data package in ~5 business days, including raw data and a standard curve.
- Direct Manufacturer Value: Eliminate distributor costs by sourcing your ELISA kits directly from our China-based manufacturing facility.
Our Commitment to Your Research
- Publication Reward: Cite this Mouse Osteopontin ELISA Kit with a link to our product page in your publication, and receive a complimentary standard ELISA kit (up to $120 value).
- 100% Satisfaction Guarantee: We stand behind our products with an unconditional replacement or refund policy if performance does not meet specifications.
- Dedicated 24-Hour Support: Our technical and customer service teams provide a clear path to resolution within one business day.
- Integrity in Partnership: We treat your research with the utmost respect, handling every sample with precision to safeguard your scientific investment.
Assay Principle & Specifications
Detection Principle: The kit employs a sandwich ELISA. Mouse Osteopontin in the sample is captured between a pre-coated antibody and an HRP-conjugated detection antibody. After washing, the TMB substrate reaction yields a blue product that turns yellow upon acid addition. The absorbance at 450 nm is directly proportional to the Osteopontin concentration.
Performance Specifications:
- Sensitivity: < 0.1 ng/mL
- Detection Range: 0.1 – 30 ng/mL
- Accuracy (Linearity): Correlation Coefficient R ≥ 0.9900
- Precision: Intra-assay CV < 10%; Inter-assay CV < 15%
- Specificity: No significant cross-reactivity with analogous proteins.
- Storage: 2-8°C, protected from light; stable for 6 months.
Sample Handling: Collect serum or plasma using standard protocols (EDTA/citrate recommended). Centrifuge at 3000×g for 10-30 minutes to clarify. Aliquot and store samples at -20°C, avoiding repeated freeze-thaw cycles. A preliminary experiment is recommended to determine the optimal dilution factor for your specific samples.
Ordering Information & Services
| Option | Description | Details |
|---|---|---|
| Mouse SPP1 ELISA Kit Purchase | Complete kit for in-laboratory use. | Contact for competitive manufacturer price. Bulk orders: < $100 (quote required). |
| Expert Testing Service | Full-service analysis performed in our lab. | ~5-day turnaround. Free with kit purchase. |
| Custom ELISA Development | Tailored assays for unique research needs. | Project-based quotation available. |
| Human Osteopontin ELISA Kit | Also available for human sample analysis. | Inquire for details and pricing. |
To buy the Mouse Osteopontin ELISA Kit or request a quote, contact Yanda Bio, your trusted direct supplier.
Research Applications & Frequently Asked Questions (FAQ)
Primary Applications (Research Use Only): Inflammatory and autoimmune disease models, bone metabolism and osteoporosis research, cancer biology (tumor metastasis, microenvironment), cardiovascular disease (atherosclerosis, vascular calcification), and wound healing studies.
Q: What is the main function of Osteopontin (SPP1)?
A: Osteopontin is a multifunctional protein involved in cell adhesion, migration, and signaling. It plays critical roles in immune regulation, inflammatory responses, biomineralization, and tumor progression, acting as a bridge between cells and the extracellular matrix.
Q: How do I determine the correct sample dilution?
A: The protocol suggests a starting dilution. For accurate results, especially with unknown sample concentrations, we recommend running a preliminary dilution experiment to ensure the measured value falls within the optimal range of the standard curve.
Q: How do I claim the free kit for a publication?
A: Upon publication of your article citing the “Mouse Osteopontin (SPP1) ELISA Kit (Yanda Bio)” with a link to our product page, send us the citation (DOI or PDF). We will promptly ship your complimentary kit.
Q: Why choose Yanda Bio as my supplier from China?
A: As a certified manufacturer, we provide authentic pricing without markups, stringent in-house quality control, direct access to technical experts, and a vast portfolio, ensuring you receive a reliable, high-value research tool.
Manufacturer’s Note
This product is for Research Use Only (RUO). It is not intended for diagnostic, therapeutic, or clinical procedures. Users are responsible for determining the suitability of the kit for their specific applications and for following all recommended safety guidelines when handling biological samples.
