Mouse RASSF6 ELISA Kit Materials supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0, 12.5,25,50, 100,200 pg/ml
Mouse RASSF6 ELISA Kit for Cancer & Cellular Signaling Research
Yanda Bio’s Mouse RAS Domain-Containing Protein 6 (RASSF6) ELISA Kit provides a sensitive and specific tool for the quantitative measurement of RASSF6 in mouse tissue lysates, cell extracts, serum, and plasma. As a member of the RASSF family of tumor suppressors, RASSF6 plays crucial roles in regulating apoptosis, cell cycle progression, and microtubule stability. This robust assay is essential for advancing research in oncology, cell biology, and developmental studies using mouse models.
As a factory-direct manufacturer with professional laboratories in China, we combine advanced assay technology with exceptional value. This Mouse RASSF6 ELISA Kit made in China is competitively priced at a retail of $120, with bulk pricing under $100 available. It is part of our extensive portfolio of over 6,000 research assays, supported by collaborations with 70+ universities worldwide.
Select the Right Tier for Your Research Needs
We offer two meticulously optimized kit formats to match your specific experimental requirements and budget. Purchase of any tier includes our complimentary expert testing service.
| Feature | Tier 1: Standard Mouse RASSF6 ELISA Kit | Tier 2: High-Sensitivity Mouse RASSF6 ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with a proprietary matrix-suppression diluent. | Biotinylated recombinant monoclonal antibody (>95% pure) paired with Streptavidin-HRP conjugate (≥500 U/mg). |
| Key Advantage | Cost-Effective & Proven Reliability. Uses a simultaneous incubation step for efficiency. The specialized diluent minimizes background from hemoglobin and lipids, ensuring stable performance for common targets. | Maximum Sensitivity & Precision. The advanced biotin-streptavidin system directly neutralizes matrix effects, offering superior signal-to-noise for low-abundance targets or challenging samples. |
| Our Selection Advice | Ideal for common research factors. Choose this tier for its excellent value and proven track record in years of experimental validation. | Recommended for precious samples or rare targets. Select this tier to ensure your experiment succeeds on the first attempt with the highest sensitivity. |
Included Expert Testing Service: Fast, Reliable Data
Accelerate your research and guarantee data quality with our professional in-house service, included with every purchase.
- Rapid Initiation: Your assay begins within 24 hours of sample receipt.
- Comprehensive Reporting: Receive a full data package in ~5 business days, including raw OD values, concentration tables, and a standard curve graph.
- Confidence in Validation: Perfect for confirming performance before establishing your in-house protocol.
Our Commitment to Your Research Success
We build lasting partnerships through concrete actions and guarantees:
- Publication Reward: Cite this Mouse RASSF6 ELISA Kit with a link to our product page in your publication, and we will send you a complimentary standard ELISA kit (up to $120 value).
- 100% Satisfaction Guarantee: We promise performance. If a kit does not meet its specifications, we offer an unconditional replacement or refund.
- 24-Hour Support Pledge: Our team provides a proposed solution within 24 hours of any inquiry.
- Guardians of Your Science: We handle every sample with the utmost precision, safeguarding the integrity of your valuable research.
Assay Principle & Technical Specifications
Detection Principle: The kit employs a validated one-step sandwich ELISA. Mouse RASSF6 is captured between an immobilized antibody and an HRP-conjugated detection antibody. The subsequent TMB reaction, measured at 450 nm, produces a signal directly proportional to the RASSF6 concentration.
Performance Specifications:
- Sensitivity: < 1.0 pg/mL.
- Detection Range: 1.0 – 200 pg/mL.
- Accuracy (Linearity): Correlation Coefficient R ≥ 0.9900.
- Precision: Intra-assay & Inter-assay Coefficient of Variation (CV) < 15%.
- Specificity: No significant cross-reactivity with analogous structures.
- Storage: 2-8°C, protected from light and moisture; 6-month shelf life.
Sample Handling: For tissue and cell samples, use appropriate lysis buffers. Clarify serum or plasma by centrifugation at 3000×g for 10-30 minutes. Aliquot and store all samples at -20°C or lower, avoiding repeated freeze-thaw cycles. A 1:5 sample dilution is typically recommended for serum/plasma.
Streamlined Assay Workflow
- Addition: Add 50 µL of standards or prepared samples to the pre-coated wells.
- Incubation: Add 100 µL of the HRP-conjugated Detection Antibody (except to blank well). Incubate at 37°C for 60 minutes.
- Wash: Wash the microplate 5 times thoroughly with the prepared wash buffer.
- Development: Add 50 µL each of Substrate A and B. Incubate at 37°C for 15 minutes in the dark.
- Measurement: Add 50 µL Stop Solution. Read the absorbance at 450 nm within 15 minutes.
Ordering Information & Services
| Option | Description | Details |
|---|---|---|
| Tier 1: Standard Kit Purchase | Cost-effective, reliable solution for routine research. | Retail: $120. Bulk: < $100 (quote required). |
| Tier 2: High-Sensitivity Kit Purchase | Premium performance for maximum sensitivity needs. | Contact for competitive manufacturer price. |
| Expert Testing Service | Full-service analysis performed in our lab. | ~5-day turnaround. Free with kit purchase. |
| Custom ELISA Development | Tailored assays for unique research targets. | Project-based quotation available. |
Ready to buy the Mouse RASSF6 ELISA Kit? Contact Yanda Bio for pricing, to request a bulk quote, or to start your free testing service.
Research Applications & Frequently Asked Questions (FAQ)
Primary Applications (Research Use Only): Cancer biology and tumor suppressor gene research, studies of apoptosis and cell cycle regulation, investigation of signaling pathways (e.g., Hippo, RAS/MAPK), and developmental biology.
Q: What is the primary biological function of RASSF6?
A: RASSF6 is a tumor suppressor protein involved in promoting apoptosis, inhibiting cell cycle progression, and regulating cytoskeletal dynamics. It often functions as a scaffold protein within key signaling networks.
Q: How do I choose between Tier 1 and Tier 2?
A: Follow our Selection Advice. For well-characterized, commonly studied targets where sample is not limiting, Tier 1 offers great value. For rare targets, low-expression samples, or extremely precious/limited samples, invest in Tier 2 for its superior sensitivity and reliability to ensure experimental success.
Q: How do I claim the free kit for a publication?
A: Once your peer-reviewed article citing the “Mouse RASSF6 ELISA Kit (Yanda Bio)” with a link to this product page is published, send us the citation details (DOI). We will promptly ship your complimentary reward.
Q: Why buy an ELISA kit made in China from Yanda Bio?
A: As a direct manufacturer, we provide authentic manufacturer pricing, stringent in-house quality control, direct technical support from our R&D team, and a vast portfolio, ensuring you receive a high-value, reliable research tool without intermediary costs.
Manufacturer’s Note
This product is for Research Use Only (RUO). It is not intended for diagnostic, therapeutic, clinical, or animal clinical procedures. Researchers are responsible for validating the kit’s suitability for their specific experimental models.
