The Mouse RNP ELISA Kit Contains
The kit is divided into 96T and 48T versions and includes the following parts：
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 8* 12strips | 8*6strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0,3,6, 12,24,48 ng/mL
Select the Right Assay Format for Your Research Goals & Budget
We provide two optimized formats for the Mouse RNP ELISA Kit to suit varying sensitivity requirements and project scopes. Purchase of any tier includes our complimentary expert sample testing service.
| Feature | Tier 1: Standard Mouse RNP ELISA Kit | Tier 2: High-Sensitivity Mouse RNP ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with a proprietary, interference-reducing diluent. | Biotinylated recombinant monoclonal antibody (>95% pure) paired with Streptavidin-HRP conjugate (≥500 U/mg). |
| Key Advantage | Proven Reliability & Exceptional Value (>99% success rate). Uses a simultaneous incubation for efficiency. The specialized diluent minimizes background from hemoglobin and lipids, ensuring robust, consistent results. | Maximum Sensitivity & Superior Specificity. The advanced biotin-streptavidin system directly neutralizes matrix effects, delivering outstanding signal-to-noise for low-abundance targets or complex samples. |
| Our Selection Advice | Ideal for common autoimmune biomarker research. This tier offers outstanding value with a retail price of $120 and bulk pricing under $100. | Recommended for precious/rare samples or detecting subtle changes. Choose this tier for the highest confidence and first-attempt success in critical experiments. |
| Best For | Routine screening, established disease models, and cost-sensitive projects. | Studies with limited sample volume, low-titer autoantibodies, or requiring the ultimate detection precision. |
Comprehensive Sample Handling Guide
Our kit supports a wide range of sample types, prepared as follows:
- Liquid Samples (Serum, Plasma, CSF, Urine): Collect using appropriate tubes. Centrifuge at 2000-3000×g for 20 minutes to clarify. Aliquot and store at -20℃ or -80℃, avoiding repeated freeze-thaw cycles.
- Tissue Homogenates: Rinse tissue with cold PBS, homogenize in PBS (typically 1:9 w/v) with protease inhibitors, and centrifuge at 5000×g for 10 minutes. Collect the supernatant.
- Cell Lysates: Harvest and wash cells. Resuspend in PBS and disrupt using sonication. Centrifuge at 3000×g for 20 minutes to collect the lysate.
- Important Note: Do not use samples containing sodium azide (NaN₃), as it inhibits HRP activity.
Complimentary Expert Testing Service
Save time and validate your assay with our professional in-house service, included with every kit purchase.
- Fast Processing: We begin analysis within 24 hours of sample receipt.
- Complete Report: Receive a detailed data package in ~5 business days, with raw OD values, concentration tables, and a standard curve.
Why Choose Yanda Bio’s RNP ELISA Kit?
- Direct Manufacturer Value: Source Mouse RNP ELISA Kit with manufacturer price directly, ensuring the best cost without intermediaries.
- Custom & Production Expertise: We offer Mouse RNP ELISA Kit customed solutions and reliable ELISA kit production.
- Publication Reward: Cite this kit with a link to our site in your publication, and receive a free standard ELISA kit.
- 100% Satisfaction Guarantee: We promise performance with an unconditional replacement or refund policy.
- 24-Hour Support: Receive a proposed solution within one business day.
Assay Principle & Technical Specifications
Detection Principle: This sandwich ELISA captures mouse RNP between an immobilized antibody and an HRP-conjugated detection antibody. The TMB substrate reaction, measured at 450 nm, produces a signal proportional to RNP concentration.
Performance Specifications:
- Sensitivity: < 1.0 ng/mL.
- Detection Range: 1.0 – 48 ng/mL.
- Accuracy: R ≥ 0.9900.
- Precision: Intra- & Inter-assay CV < 15%.
- Specificity: High specificity with minimal cross-reactivity.
- Storage: 2-8℃; 6-month shelf life.
Streamlined Assay Workflow
- Add 50 µL of standards or prepared samples (10µL sample + 40µL diluent) to wells.
- Add 100 µL HRP-conjugated Detection Antibody (except blank). Incubate at 37℃ for 60 min.
- Wash plate 5 times thoroughly.
- Add 50 µL each of Substrate A & B. Incubate at 37℃ for 15 min (dark).
- Add 50 µL Stop Solution. Read absorbance at 450 nm within 15 min.
Calculation: Generate a standard curve from the provided standards to determine sample concentrations (multiply result by dilution factor 5).
Ordering, Custom Services & Support
| Option | Description | Details |
|---|---|---|
| Tier 1: Standard Kit | Cost-effective solution for common research needs. | Retail: $120. Bulk: <$100 (quote required). |
| Tier 2: High-Sensitivity Kit | Premium performance for critical applications. | Contact for manufacturer price. |
| Expert Testing Service | Full-service analysis and reporting. | Free with kit purchase. |
| Custom ELISA Development | Tailored Mouse RNP ELISA Kit customed to your specifications. | Project-based quote. |
To buy this reliable, cost-effective Mouse RNP ELISA Kit, contact Yanda Bio today for pricing or to discuss custom options.
Research Applications & FAQ
Primary Applications (RUO): Autoimmune disease research (SLE, MCTD models), studies of anti-RNP autoantibodies, molecular biology research on RNA-protein complexes, and virology (viral RNP complexes).
Q: What specific RNP does this kit detect?
A: This Murine Ribonucleoprotein ELISA kit is designed to detect a common RNP complex or component targeted in autoimmune research. For exact specificity (e.g., U1-snRNP), please contact our technical support for the latest validation data.
Q: How does this kit compare to other suppliers?
A: Our Mouse RNP ELISA Kit offers a cost-effective elisa kits alternative with comparable performance, direct manufacturer support, bulk pricing under $100, and included value-added services like free testing.
Q: Can this kit be used for other species?
A: It is validated for mouse samples. For other species, please inquire about our custom service for cross-reactivity testing or assay development.
Q: How do I claim the free publication kit?
A: Send us the citation (DOI) of your published article that cites “Mouse RNP ELISA Kit (Yanda Bio)” and links to our product page.
Manufacturer’s Note
For Research Use Only (RUO). Not for diagnostic use.
