Why Our Technology Stands Out
To ensure the highest data integrity, this kit employs an advanced detection system:
- High-Purity Biotinylated Antibody: Utilizes a recombinant monoclonal antibody with >95% purity, ensuring exceptional specificity and batch-to-batch consistency.
- Powerful Signal Amplification: Incorporates a high-activity Streptavidin-HRP complex (≥500 U/mg). This robust biotin-streptavidin interaction provides significant signal amplification.
- Minimized Matrix Interference: This combined system is engineered to directly circumvent sample matrix effects and background interference, resulting in cleaner signals, superior sensitivity (<1.0 nmol/L), and more accurate quantification in complex biological fluids.
Complete Kit Specifications & Performance
Key Performance Data
| Parameter | Specification |
|---|---|
| Principle | One-Step Sandwich ELISA with Biotin-Streptavidin Amplification |
| Accuracy (Linearity) | Correlation Coefficient R ≥ 0.9900 |
| Sensitivity | < 1.0 nmol/L |
| Detection Range | 1.0 – 3200 nmol/L |
| Specificity | No significant cross-reactivity with analogous metabolites |
| Precision | Intra-assay & Inter-assay CV < 15% |
| Sample Volume | 10 µL (requires 1:5 dilution) |
| Total Incubation Time | ~75 minutes |
| Shelf Life & Storage | 6 months at 2-8°C, protected from light |
Standard Curve & Components
Standard Concentrations (S0-S5): 0, 200, 400, 800, 1600, 3200 nmol/L.
Kit Contents (96T / 48T):
- Pre-coated Microplate (8 / 4 strips)
- Standards (0.3mL x 6 tubes)
- Sample Diluent (6mL / 3mL)
- Biotinylated Detection Antibody (10mL / 5mL)
- Streptavidin-HRP Conjugate (10mL / 5mL)
- 20X Wash Buffer (25mL / 15mL)
- Substrate A & B (6mL / 3mL each)
- Stop Solution (6mL / 3mL)
- Plate Sealers, Detailed Protocol, Storage Bag
Expert-Recommended Workflow
Sample Preparation:
Collect serum or plasma using standard procedures. For all samples, a 1:5 pre-dilution is required: mix 10 µL of sample with 40 µL of the provided Sample Diluent. Aliquot and store samples at -20°C to avoid freeze-thaw degradation.
Assay Procedure Summary:
- Add: Pipette 50 µL of standards or diluted samples into wells.
- Incubate: Add 100 µL of the prepared detection complex (Biotin-Ab + SA-HRP). Incubate at 37°C for 60 min.
- Wash: Perform 5 wash cycles using the prepared 1X Wash Buffer.
- Develop: Add 50 µL each of Substrate A and B. Incubate at 37°C for 15 min in the dark.
- Stop & Read: Add 50 µL Stop Solution. Measure absorbance at 450 nm within 15 minutes.
Calculation: Plot the standard curve and interpolate sample values. Multiply the result by the dilution factor (5) for final concentration.
Ordering, Support & Partnership Opportunities
Pricing & Purchase Options
- Retail Purchase: $120 per kit. Ideal for individual researchers and pilot studies.
- Bulk/Wholesale Purchase: Contact for a quote below $100 per kit. Tailored for core facilities, large labs, and long-term projects.
- Custom Kit Development: Our expert team can modify the assay range, sensitivity, or compatibility for your unique application.
- Free Sample Testing Service: Validate the kit’s performance with your specific samples at no cost.
Our Commitment to Research
As a factory-direct manufacturer with proprietary laboratories, Yanda Bio controls every production step, ensuring quality and affordability. Our long-standing partnerships with over 70 academic institutions attest to the reliability and performance of our assays.
Elevate your research on erythrocyte metabolism and oxygen transport. Contact us today to purchase, request a bulk quote, or initiate your free testing service.
Research Applications & Technical Support
Primary Applications (Research Use Only):
- Hematology & Transfusion Science: Monitor 2,3-DPG levels in stored blood, study hemoglobin oxygen-release capacity.
- Metabolic & Hypoxia Research: Investigate metabolic adaptations in high-altitude physiology, anemia, and related disorders.
- Sports Science: Assess erythrocyte metabolic responses to training and performance.
Frequently Asked Questions (FAQ):
Q: Why is the biotin-streptavidin system superior for 2,3-DPG detection?
A: This system provides powerful signal amplification, drastically improving the signal-to-noise ratio. It is particularly effective at mitigating non-specific background common in complex matrices like hemolysates, leading to more precise and reliable low-concentration measurements.
Q: What is your lead time for bulk orders?
A: As the direct manufacturer, we maintain efficient inventory and production. Typical lead times are 5-10 business days, with expedited options available.
Q: Do you provide technical documentation for grants or publications?
A: Yes. We supply comprehensive validation data, performance certificates, and detailed protocols suitable for grant applications and methodological sections in publications.
Q: Can this kit be used for diagnostic purposes?
A: No. This product is sold for Research Use Only (RUO). It is not intended for use in clinical diagnostic procedures.
Disclaimer
This product is for research purposes only. It is not validated or approved for diagnostic, therapeutic, or clinical use. The user assumes all responsibility for compliance with applicable regulations and for the consequences of protocol deviations.
