Product Introduction: The Significance of 3-Indolepropionic Acid (3-IPA)

3-Indolepropionic Acid (3-IPA) is a potent microbial metabolite derived from dietary tryptophan, recognized for its strong antioxidant and neuroprotective properties. As a key mediator in the gut-brain axis, accurate quantification of Rat 3-IPA is crucial for advancing research in metabolic disorders, neurodegenerative diseases, inflammatory conditions, and microbiome-host interactions. Understanding its concentration dynamics provides invaluable insights into systemic health and disease mechanisms.

Yanda Bio’s Rat 3-Perindopril acid (3-IPA) ELISA Kit is engineered to deliver exceptional accuracy and reliability for this important biomarker. As a dedicated manufacturer in the ELISA Kit production field, with an expansive portfolio of over 6,000 detectable targets and proven collaborations with more than 60 universities, we are committed to empowering scientists with high-performance, cost-effective rat ELISA Kit solutions that accelerate discovery.

Uncompromised Quality, Exceptional Value: Our Production Advantage

A critical determinant of ELISA kit performance is the quality of its core immunoreagents—specifically, the affinity and specificity of the antibodies. Premium-priced kits often leverage superior antibody quality to justify their cost.

Our Quality Proposition: At Yanda Bio, we integrate high-affinity, species-specific antibodies that meet rigorous performance benchmarks, comparable to those utilized by leading international suppliers. Through our optimized and scalable ELISA Kit production processes, we deliver this premium quality within a truly low-cost ELISA Kit framework. By reducing distribution overhead and operating with high efficiency, we make high-end sensitivity and specificity accessible for every research budget, offering significant savings compared to major brands.

Two Optimized Formats: Tailored Precision for Every Research Goal

To address diverse experimental requirements—from high-throughput screening to analysis of limited precious samples—we offer this assay in two strategically designed formats.

Format 1: High-Efficiency Standard Kit

Ideal for routine, reliable quantification of 3-IPA in standard research settings. This format features a streamlined one-step incubation procedure, significantly reducing hands-on time. It includes a proprietary matrix optimization buffer specifically formulated to mitigate interference from hemoglobin, lipids, and other common sample constituents. This ensures exceptionally low background noise and high signal-to-noise ratios, leading to reproducible and trustworthy data. Validated over countless experiments, this format boasts a success rate exceeding 99%.

• Standard Pricing: Approximately $120 (retail). Under $100 per kit for volume, wholesale, or institutional purchase programs.

Format 2: Ultra-Sensitive Detection Kit

Designed for the most demanding applications where analyte concentration is expected to be extremely low or sample material is scarce and irreplaceable (e.g., biopsy samples, rare animal models). This advanced system employs a biotinylated recombinant monoclonal antibody (purity >95%) paired with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This powerful signal amplification cascade maximizes detection sensitivity, effectively neutralizes complex matrix effects, and is engineered to guarantee definitive results in pivotal, one-attempt experiments.

Selection Guide for Researchers:

• Choose Format 1 for cost-effective, high-confidence measurement in studies with ample sample volume.
• Choose Format 2 when your research involves trace-level detection, minimal sample quantities, or requires the ultimate level of assay robustness to overcome challenging sample matrices.

Assay Principle: Reliable Sandwich ELISA Technology

This kit utilizes a robust sandwich enzyme-linked immunosorbent assay (ELISA) design. A highly specific capture antibody for Rat 3-IPA is immobilized on the microplate. During incubation, the 3-IPA present in standards or samples binds to these coated antibodies. After washing, an HRP (Horseradish Peroxidase)-conjugated detection antibody is added, forming a complete “antibody-antigen-antibody” complex. Following another wash step to remove unbound material, a TMB (3,3′,5,5′-Tetramethylbenzidine) substrate solution is added. The HRP enzyme catalyzes the conversion of TMB to a blue product, which turns yellow upon acidification. The intensity of this yellow color, measured at 450 nm, is directly proportional to the concentration of 3-IPA in the original sample.

Comprehensive Support & Guarantees for Your Research Success

• Direct Savings on Premium Components: Access top-tier immunoassay performance through our efficient, cost-effective rat ELISA Kit supply model, avoiding traditional brand markups.
• Free Expert-Assisted Testing: Purchase any Yanda Bio ELISA kit and receive a complimentary data generation service. Provide your prepared samples, and our application specialists will perform the assay and deliver the results.
• Publication Incentive Program: Recognize the use of our product in your peer-reviewed publication with a citation and direct link to this product page, and we will gift you a free standard ELISA kit as a token of our appreciation.
• 100% Satisfaction & Performance Guarantee: We stand behind our products with full confidence. If the kit’s performance does not meet the specifications detailed in this document, we offer an unconditional replacement or full refund.
• Responsive, Expert Technical Support: Our dedicated support team is committed to providing a detailed, actionable response to any technical or application inquiry within 24 hours.
• Custom Assay Development Services: For targets beyond our extensive catalog, explore our end-to-end custom ELISA kit design and production capabilities.

Detailed Specifications & Performance Data

• Accuracy & Linearity: The standard curve exhibits a linear regression correlation coefficient (R) of ≥ 0.9900, ensuring excellent agreement with expected values.
• Precision: Both intra-assay (within-run) and inter-assay (run-to-run) precision are demonstrated with Coefficients of Variation (CV) consistently less than 15%.
• Specificity: The assay shows no significant cross-reactivity with other common indole derivatives or structurally similar soluble compounds.
• Sample Compatibility: Fully validated for use with rat-derived samples, including serum, plasma (EDTA, citrate, heparin anticoagulants), cell culture supernatant, and tissue homogenates.
• Stability & Shelf Life: The complete kit is stable for 6 months from the date of manufacture when stored continuously at the recommended 2-8°C.