Rat 5-Alpha Reductase (5AR) ELISA Kit supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0,5, 10,20,40,80 U/mL
Understanding 5-Alpha Reductase (5AR) in Research
5-Alpha Reductase (5AR) is a critical intracellular enzyme responsible for converting testosterone into the more potent androgen, dihydrotestosterone (DHT). This conversion is fundamental to processes in sexual development, prostate physiology, and hair growth regulation. Accurate quantification of Rat 5AR levels is therefore essential for preclinical studies focused on endocrinology, benign prostatic hyperplasia (BPH), alopecia, and the metabolic pathways of steroid hormones.
Yanda Bio’s Rat 5-Alpha Reductase (5AR) ELISA Kit is meticulously designed to provide researchers with a robust and reproducible tool for this important target. As a leader in ELISA Kit production, with expertise spanning over 6,000 detectable factors and sustained collaborations with more than 60 universities, we are dedicated to supplying high-fidelity, affordable rat ELISA Kit solutions that support rigorous scientific inquiry without exceeding budgetary constraints.
Premium Performance at an Accessible, Affordable Price
A cornerstone of reliable ELISA data is the quality of the core immuno-reagents. High-cost kits typically invest in superior antibody specificity and optimized assay buffers to ensure performance.
Our Value Proposition: We integrate high-specificity, high-affinity antibodies that meet rigorous performance standards, comparable to those utilized by leading international brands. Through our streamlined and efficient ELISA Kit production and direct-to-researcher model, we deliver this premium core quality within a genuinely low-cost ELISA Kit structure. This approach allows us to offer a truly cheap ELISA Kit alternative without the typical compromises in sensitivity or reliability, providing exceptional value for hormone and enzyme research.
Two Optimized Kit Formats for Diverse Research Applications
To serve the varied needs of the scientific community—from high-throughput studies to analysis of scarce samples—we offer this assay in two distinct, performance-calibrated formats.
Format 1: Standard High-Value Kit
Engineered for reliable, routine quantification of 5AR. This format features an efficient one-step incubation protocol, minimizing hands-on time. It includes a proprietary sample diluent specifically formulated to neutralize interference from common matrix components like hemoglobin and lipids, ensuring a stable baseline and superior signal-to-noise ratio. This format is validated for a success rate above 99% in established applications and represents our most cost-effective and affordable entry point.
- Typical Pricing: Around $120 (retail). Less than $100 per kit for volume, wholesale, or institutional purchase programs.
Format 2: Advanced High-Sensitivity Kit
Developed for demanding research scenarios involving low-abundance enzyme expression, limited sample volumes, or complex biological matrices. This advanced system employs a biotinylated recombinant monoclonal antibody (purity >95%) paired with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This sophisticated signal amplification cascade maximizes detection sensitivity, effectively circumvents matrix effects, and is engineered to guarantee success in critical, one-attempt experiments with irreplaceable samples.
Selection Guide for Optimal Results:
- Choose Format 1 for robust and economical analysis in studies where sample material is not limiting.
- Choose Format 2 when your research requires the ultimate detection limit for trace-level analysis, involves precious or minimal sample quantities, or demands maximum assay robustness to overcome challenging sample backgrounds.
Assay Principle: Reliable Sandwich ELISA Technology
This kit operates on a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) principle. A highly specific antibody for Rat 5-Alpha Reductase is pre-coated onto the microplate. During incubation, the 5AR enzyme present in standards or samples binds to these immobilized antibodies. After washing, a Horseradish Peroxidase (HRP)-conjugated detection antibody is added, forming a complete “antibody-antigen-antibody” complex. Following another wash step to remove unbound material, a TMB substrate solution is added. The HRP enzyme catalyzes the conversion of TMB to a blue product, which turns yellow upon acidification. The intensity of this yellow color, measured at 450 nm, is directly proportional to the concentration of 5AR in the original sample.
Commitment to Your Research Success
- Direct Savings on Quality: Access premium immunoassay components through our efficient, cost-effective rat ELISA Kit model, offering significant savings versus premium brands.
- Complimentary Expert Testing Service: Purchase any Yanda Bio ELISA kit and receive FREE expert-assisted testing. Provide your prepared samples, and our specialists will run the assay and deliver validated results.
- Publication Incentive Program: Acknowledge the use of our product with a citation and direct link in your peer-reviewed publication to receive a complimentary standard ELISA kit.
- 100% Satisfaction & Performance Guarantee: We stand firmly behind our承诺 with an unconditional promise: if product performance does not meet published specifications, you are eligible for a prompt replacement or full refund.
- Dedicated 24-Hour Support: Our technical support team is committed to providing a detailed, actionable response to any inquiry within one business day.
- Custom Assay Solutions: For specialized targets beyond our expansive catalog, explore our comprehensive custom ELISA kit design and development services.
Detailed Specifications & Performance Data
- Accuracy & Linearity: The standard curve demonstrates a linear regression correlation coefficient (R) of ≥ 0.9900, confirming excellent agreement with expected values.
- Precision: Both intra-assay (within-run) and inter-assay (run-to-run) Coefficients of Variation (CV) are consistently less than 15%.
- Specificity: The assay shows no significant cross-reactivity with other structurally similar soluble enzymes or proteins.
- Sample Compatibility: Validated for use with rat serum, plasma (EDTA, citrate, heparin), cell lysates, and tissue homogenates.
- Stability & Shelf Life: The complete kit is stable for 6 months from the date of manufacture when stored continuously at 2-8°C.
