Rat 1,25 DHVD3 ELISA Kit Materials supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0,3,6, 12,24,48 ng/ml
Product Overview: Targeting the Active Vitamin D Metabolite
1,25-Dihydroxyvitamin D3 (Calcitriol or 1,25 DHVD3) is the hormonally active form of Vitamin D, serving as a critical regulator of calcium-phosphate homeostasis, bone metabolism, and immune system function. Precise measurement of Rat 1,25 DHVD3 is fundamental for research in renal physiology, osteoporosis, autoimmune diseases, and endocrine disorders, where its levels provide direct insight into metabolic activity.
Yanda Bio’s Rat 1,25-Dihydroxyvitamin D3 ELISA Kit is meticulously developed to offer researchers a reliable and accurate tool for quantifying this key hormone. As a specialist in ELISA Kit production, with a comprehensive portfolio of over 6,000 detectable targets and established partnerships with more than 60 academic institutions, we deliver high-performance, cost-effective rat ELISA Kit solutions designed to meet rigorous research standards.
Exceptional Value Without Compromising Performance
The reliability of an ELISA kit hinges on the quality of its antibodies and the optimization of its assay chemistry. Premium international kits command high prices for this guaranteed performance.
Our Commitment to Accessible Quality: We source high-specificity, high-affinity antibodies that meet exacting standards, ensuring core reagent parity with leading brands. By refining our manufacturing and supply chain logistics, we integrate these premium components into an efficient ELISA Kit production model. The result is a genuinely low-cost ELISA Kit that delivers the sensitivity, specificity, and reproducibility essential for critical endocrine research, at a fraction of the cost of major commercial alternatives.
Dual-Format Flexibility: Choose Based on Your Experimental Priorities
To accommodate varying research needs—from routine screening to high-stakes, sample-limited studies—we offer this kit in two distinct, performance-calibrated formats.
Format A: High-Economy Standard Kit
Optimized for accurate and reliable quantification in standard research settings with sufficient sample volume. This format utilizes a simplified one-step incubation protocol, significantly reducing procedural time. A proprietary matrix optimization diluent is included to effectively minimize interference from common serum components like hemoglobin and lipids, ensuring low background and excellent inter-assay consistency. Validated across numerous applications, this format demonstrates a success rate exceeding 99%.
- Typical Investment: Approximately $120 (retail). Less than $100 per unit for bulk or wholesale purchase agreements.
Format B: Advanced High-Sensitivity Kit
Engineered for the most challenging scenarios, such as detecting very low hormone concentrations or analyzing irreplaceable or minute sample volumes (e.g., from pediatric or longitudinal rodent studies). This sophisticated format employs a biotinylated recombinant monoclonal antibody (purity >95%) in conjunction with a high-activity Streptavidin-HRP complex (≥500 U/mg). This powerful signal amplification system maximizes detection limits, robustly counters matrix effects, and is crafted to ensure definitive results in pivotal experiments where repetition is not an option.
Guidance for Selection:
- Select Format A for robust, budget-conscious analysis in studies with ample sample availability.
- Select Format B when your project requires ultimate sensitivity for trace-level detection, involves precious or limited samples, or demands maximum assay robustness against complex biological matrices.
Assay Methodology: Robust Sandwich ELISA Design
This kit is based on a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) principle. A monoclonal antibody specific for Rat 1,25 DHVD3 is pre-coated onto the microplate. During incubation, the active vitamin D metabolite present in standards or samples binds to these immobilized antibodies. Following a wash step, a Horseradish Peroxidase (HRP)-conjugated detection antibody is added to form an antibody-antigen-antibody “sandwich” complex. After another wash to remove unbound conjugate, a TMB substrate solution is added. The enzymatic reaction yields a blue color, which turns yellow upon acidification. The intensity of this yellow color, measured at 450 nm, is directly proportional to the concentration of 1,25 DHVD3 in the original sample.
Comprehensive Researcher Support & Assurance
- Direct Value from Manufacturer: Access premium-grade assay components through our streamlined cost-effective rat ELISA Kit supply chain, eliminating intermediary markups.
- Free Expert-Assisted Testing Service: Purchase any Yanda Bio ELISA kit and qualify for complimentary data generation. Submit your samples, and our application specialists will perform the assay and return precise results.
- Publication Acknowledgement Program: Cite our product and link to this page in your peer-reviewed manuscript to receive a free standard ELISA kit as our contribution to your research success.
- 100% Performance Guarantee: We stand firmly behind our承诺. If the kit does not perform as specified, we offer an unconditional replacement or full refund.
- Dedicated 24-Hour Technical Support: Our expert support team is committed to providing a detailed, actionable response to any inquiry within one business day.
- Custom Assay Development: For specialized research needs beyond our vast catalog, inquire about our end-to-end custom ELISA kit design and production services.
Detailed Performance Specifications
- Accuracy & Linearity: The standard curve exhibits a linear regression correlation coefficient (R) of ≥ 0.9900, confirming excellent dose-response agreement.
- Precision: Both intra-assay (within plate) and inter-assay (between plates) coefficients of variation (CV) are consistently below 15%, ensuring reproducible results.
- Specificity: Demonstrates negligible cross-reactivity with other vitamin D metabolites (e.g., 25-OH-Vitamin D3) and structurally related compounds.
- Sample Compatibility: Validated for use with rat serum and plasma samples. Proper sample handling and storage are recommended for optimal recovery.
- Kit Stability & Storage: The complete kit is stable for 6 months from the date of manufacture when stored continuously at 2-8°C.
Ordering & Contact Details
To order this Rat 1,25-Dihydroxyvitamin D3 (1,25 DHVD3) ELISA Kit for sale, or to discuss volume pricing, specific project requirements, and custom service options, please contact our sales team .
