Rat HMGCR ELISA Kit Materials supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0,5, 10,20,40,80 U/ L
Product Overview: Targeting the Master Regulator of Cholesterol Biosynthesis
3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR) is the rate-limiting and key regulated enzyme in the mevalonate pathway, which is responsible for endogenous cholesterol synthesis. As the primary target of statin drugs, precise quantification of Rat HMGCR levels and activity is fundamental for research in hyperlipidemia, cardiovascular disease, metabolic syndrome, and pharmacological studies of lipid-lowering agents. Our unique kit design allows for the assessment of both enzyme concentration and functional activity, providing a comprehensive view of cholesterol synthesis regulation.
Yanda Bio’s Rat HMGCR ELISA Kit delivers precise and reliable data for this critical metabolic enzyme. As a leader in ELISA Kit production, with expertise across 6,000+ research targets and validated through collaborations with over 60 academic institutions, we specialize in providing high-fidelity, cost-effective rat ELISA Kit solutions for demanding metabolic and pharmacological research.
Advanced Immunoassay Quality at a Highly Competitive Price
Accurate enzyme quantification demands antibodies of exceptional specificity to distinguish the target from complex cellular matrices. Premium brands often set the standard but at a significant cost premium.
Our Value Proposition: We employ high-specificity antibodies against Rat HMGCR that meet rigorous validation criteria for both concentration and activity assays, ensuring performance parity with leading suppliers. Through our optimized, scalable ELISA Kit production and a streamlined supply chain, we deliver this advanced assay capability at a truly affordable rat ELISA Kit price point. This makes our product a genuinely cheap ELISA Kit alternative, offering a low price without compromising the accuracy required for pivotal cholesterol metabolism studies.
Technical Versatility: While this kit utilizes our optimized sandwich ELISA format for superior sensitivity and specificity, our development team can also support competitive ELISA and other assay formats. Contact us to discuss the best methodological approach for your specific application.
Two Optimized Assay Tiers: Precision Engineered for Your Research Needs
To support a wide spectrum of experimental designs—from large-scale screening to high-stakes analysis of rare samples—we offer this critical assay in two performance-calibrated formats.
Tier 1: Standard High-Efficiency Kit
Ideal for robust, routine analysis of HMGCR in standard research models. This tier utilizes a streamlined one-step incubation protocol and a proprietary matrix optimization buffer. The buffer is specifically formulated to mitigate interference from hemoglobin, lipids, and other cellular components, ensuring exceptional signal-to-noise ratio and inter-assay reproducibility. With validation data showing a success rate exceeding 99%, this represents our most accessible and low-cost ELISA Kit solution.
- Typical Pricing: Around $120 (retail). Bulk order pricing begins under $100 per kit, providing outstanding value for research groups.
Tier 2: Advanced Ultra-Sensitive Kit
Designed for the most challenging applications, such as detecting subtle changes in HMGCR expression/activity, working with minute sample volumes (e.g., fine needle aspirates), or analyzing irreplaceable tissues. This system features a biotinylated recombinant monoclonal antibody (purity >95%) coupled with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This powerful signal amplification platform delivers maximum sensitivity, robustly neutralizes matrix effects, and is engineered for guaranteed success in critical experiments where sample is limited.
Selection Guide for Researchers:
- Select Tier 1 for reliable, high-value analysis in studies with sufficient sample material.
- Select Tier 2 when your project requires the ultimate detection limit, involves trace-level quantification, or demands maximum assay robustness for complex or precious samples.
Assay Principle: Dual-Purpose Sandwich ELISA Methodology
This kit is based on a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) principle. A capture antibody specific for Rat HMGCR is immobilized on the microplate. During incubation, the HMGCR enzyme present in standards and prepared samples binds to these antibodies. Following a wash, an HRP-conjugated detection antibody is added to form an antibody-antigen-antibody complex. After another wash, TMB substrate is added. The HRP catalyzes a color change to blue, which turns yellow upon stopping. The optical density (OD) at 450 nm is directly proportional to the amount of HMGCR bound, allowing calculation of both sample concentration and, with appropriate controls and protocols, relative enzymatic activity.
Comprehensive Support & Unmatched Guarantees
- Superior Value: Access premium-grade assay components and dual-measurement capability through our efficient, cost-effective rat ELISA Kit model.
- Free Expert Testing Service: Purchase any Yanda Bio ELISA kit and receive FREE expert-assisted analysis. Submit your samples, and we will return comprehensive data.
- Publication Recognition Program: Cite our kit and link to our product page in your publication to receive a free standard ELISA kit.
- 100% Performance Guarantee: We stand firmly behind our products. If performance does not meet the published specifications, we offer an unconditional replacement or full refund.
- Responsive 24-Hour Support: Our technical team provides expert consultation and aims to deliver a solution-oriented response within one business day.
- Full-Cycle Custom Development: For novel targets or specific assay formats, we offer end-to-end custom ELISA kit services.
Detailed Performance Specifications
- Accuracy & Linearity: Standard curve linear regression correlation coefficient (R) ≥ 0.9900.
- Precision: Intra-assay and inter-assay Coefficients of Variation (CV) < 15%.
- Specificity: No significant cross-reactivity with other related metabolic enzymes.
- Sample Compatibility: Validated for rat serum, plasma (EDTA, citrate, heparin), tissue homogenates (especially liver), and cell lysates.
- Kit Stability: Stable for 6 months when stored continuously at 2-8°C.
Ordering & Contact Details
To order this Rat HMGCR ELISA Kit for sale, or to discuss bulk pricing, specific application protocols, and custom services, please contact our sales team.
