Rat 5-HT2BR ELISA Kit Materials supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0 、15 、30 、60 、120 、240 pg/mL
Product Overview: Targeting the 5-HT2B Receptor
The 5-Hydroxytryptamine Receptor 2B (5-HT2BR) is a G-protein coupled receptor (GPCR) critical in mediating serotonin’s effects on cardiovascular function, mood regulation, and gastrointestinal motility. Dysregulation of this receptor is implicated in cardiac valvulopathy, pulmonary hypertension, and central nervous system disorders. Precise measurement of Rat 5-HT2BR levels is therefore vital for preclinical research in cardiology, neuropharmacology, and drug safety assessment.
Yanda Bio’s Rat 5-HT2BR ELISA Kit delivers highly specific and reproducible quantification for this important therapeutic target. As an expert manufacturer with a portfolio of over 6,000 detectable targets and deep-rooted collaborations with more than 60 domestic universities, we provide rigorously validated, cost-effective rat ELISA Kit solutions to support your groundbreaking research.
Premium Components, Unbeatable Value in ELISA Kit Production
The cornerstone of any reliable ELISA is the quality of its antibodies. High-priced commercial kits often utilize high-affinity, well-characterized antibodies to ensure performance.
Our Quality Commitment: We source high-specificity antibodies comparable in quality to those used by leading international suppliers. By integrating these premium components into our optimized, scalable ELISA Kit production process, we eliminate unnecessary overheads. The result is a genuinely low-cost ELISA Kit that does not compromise on the sensitivity or specificity required for accurate GPCR research, offering substantial savings compared to premium brands.
Dual-Assay Formats: Optimized for Your Specific Research Scenario
Recognizing the diverse needs of researchers, we offer this kit in two distinct, performance-optimized formats.
Format A: High-Value Standard Kit
Designed for robust, routine quantification of 5-HT2BR expression in standard sample types. This format employs an efficient one-step incubation, reducing total hands-on time. A proprietary sample diluent is included to effectively neutralize interference from common matrix components (e.g., hemoglobin, lipids), ensuring low background and excellent reproducibility. With a >99% validated success rate, it represents the most cost-effective entry point for high-quality data.
- Typical Pricing: Approximately $120 (retail). Under $100 per kit for wholesale or institutional volume purchases.
Format B: Advanced Sensitivity & Specificity Kit
Engineered for challenging applications involving low-abundance receptor expression, rare samples, or complex matrices (e.g., tissue homogenates). This format utilizes a biotinylated recombinant monoclonal antibody (purity >95%) in conjunction with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This sophisticated signal amplification system delivers exceptional sensitivity, robustly overcomes matrix effects, and is designed to ensure first-attempt success in your most critical experiments.
Guidance for Selection:
- Select Format A for reliable and economical analysis in studies with ample sample material.
- Select Format B when working with trace protein levels, limited/irreplaceable samples, or when your protocol demands the highest possible detection power and specificity.
Pro-Tip: If the expected sample concentration is unknown, we recommend a preliminary dilution experiment to determine the optimal range and avoid a potential prozone (hook) effect, ensuring result accuracy.
Assay Methodology: Sandwich ELISA Principle
This kit operates on a solid-phase sandwich enzyme-linked immunosorbent assay principle. A monoclonal antibody specific for Rat 5-HT2BR is pre-coated onto the microplate. Standards and samples are pipetted into the wells, allowing the target receptor protein to bind. After washing, an HRP-conjugated detection antibody is added, forming an immobilized complex. Following a second wash, TMB substrate is added to develop a colorimetric signal proportional to the amount of bound 5-HT2BR. The reaction is stopped, and the optical density is measured at 450nm for precise quantification.
Why Researchers Choose Yanda Bio ELISA Kits
- Direct Access to Quality: Obtain research-grade kits featuring high-performance antibodies through our streamlined, cost-effective rat ELISA Kit model.
- Complimentary Expert Analysis: Purchase any Yanda Bio ELISA kit and qualify for FREE testing service—provide your samples, and our specialists will deliver the data.
- Publication Recognition Program: Acknowledge our product with a citation and direct link in your manuscript to receive a free standard ELISA kit of your choice.
- Unconditional Quality Guarantee: We stand firmly behind our承诺. Should the product fail to perform as stated in the specifications, we offer a no-questions-asked replacement or full refund.
- Rapid, Reliable Support: Our dedicated technical team is committed to providing a detailed response and actionable solution within 24 hours of your inquiry.
- Tailored Assay Development: Explore our comprehensive custom ELISA kit services for targets beyond our expansive catalog of 6,000+ factors.
Detailed Performance Specifications
- Accuracy: Standard curve linear regression demonstrates a correlation coefficient (R) of ≥ 0.9900.
- Precision: Intra-assay Coefficient of Variation (CV) < 10%; Inter-assay CV < 15%.
- Specificity: No significant cross-reactivity with other soluble serotonin receptor subtypes or analogous structures.
- Sample Compatibility: Validated for rat serum, plasma (EDTA, citrate, heparin), cell lysates, and tissue homogenates.
- Stability: The complete kit is stable for 6 months when stored continuously at 2-8°C.
