Rat SERT ELISA Kit Materials supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0 、15 、30 、60 、120 、240 pg/mL
Product Overview: Targeting the Serotonin Reuptake Regulator
The Serotonin Transport Protein (SERT or SLC6A4) is a critical transmembrane protein responsible for the reuptake of serotonin (5-HT) from the synaptic cleft back into the presynaptic neuron, thereby terminating its signal. As the primary pharmacological target for Selective Serotonin Reuptake Inhibitors (SSRIs), precise quantification of Rat SERT density and expression is fundamental for research in depression, anxiety, obsessive-compulsive disorder, neuropharmacology, and gastrointestinal neurobiology. Reliable measurement of SERT levels provides direct insight into serotonergic neurotransmission integrity and drug mechanism of action.
Yanda Bio’s Rat Serotonin Transport Protein (SERT) ELISA Kit is meticulously validated to offer researchers a sensitive and specific tool for this pivotal target. As an established expert in ELISA Kit production with a portfolio spanning over 6,000 detectable factors and supported by collaborative research with more than 60 Chinese universities, we are dedicated to providing high-performance, cost-effective rat ELISA Kit solutions for cutting-edge neuroscience and psychiatric research.
Research-Grade Specificity at a Truly Accessible Cost
Accurate quantification of membrane transport proteins like SERT demands antibodies with exceptional specificity to ensure no cross-reactivity with similar solute carriers. This requirement often results in high costs for premium commercial kits.
Our Value Commitment: We utilize high-affinity, highly specific antibodies against Rat SERT that are rigorously validated for precise target recognition, ensuring performance standards that match leading international suppliers. Through our optimized, in-house ELISA Kit production and a streamlined direct-to-researcher model, we integrate this quality into an exceptionally affordable rat ELISA Kit. This approach delivers a genuinely low-price ELISA Kit alternative, providing substantial cost savings—often 40-60% less than major brands—without compromising the data integrity required for conclusive neurobiological studies.
Methodological Flexibility: While this kit employs our optimized sandwich ELISA format for optimal sensitivity in detecting SERT protein levels, our technical team is proficient in developing assays using competitive ELISA or other formats. Contact us to discuss the optimal design for your specific research question.
Two Performance-Tiered Formats: Optimized for Every Experimental Need
To serve the full spectrum of research applications—from exploratory screening to definitive analysis of precious samples—we offer this essential assay in two strategically designed formats.
Tier 1: Standard High-Efficiency Kit
Designed for robust and reliable quantification of SERT in standard research models where sample is not limiting. This tier employs a simplified one-step incubation and a proprietary matrix optimization diluent. This diluent is critical for mitigating interference from high lipid content in neural tissues, hemoglobin, and other complex sample matrices, ensuring a low, stable background and high reproducibility. With extensive validation demonstrating a >99% success rate, this tier represents our most accessible and cheap ELISA Kit solution.
- Typical Pricing: Approximately $120 (retail). Significant discounts apply for bulk orders, with prices starting under $100 per kit.
Tier 2: Advanced Ultra-Sensitive Kit
Engineered for the most demanding research scenarios. This includes detecting subtle changes in SERT expression in response to stimuli or pharmaceuticals, working with extremely limited sample quantities (e.g., micro-dissected brain regions, biopsy samples), or analyzing archival tissues. This system features a biotinylated recombinant monoclonal antibody (purity >95%) paired with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This powerful signal amplification platform achieves maximum sensitivity, directly overcomes challenging matrix effects, and is engineered for guaranteed success in critical, one-attempt experiments.
Guidance for Selection:
- Select Tier 1 for cost-effective, high-confidence analysis in studies with sufficient tissue or sample material.
- Select Tier 2 when your research requires the ultimate detection limit for low-abundance targets, involves irreplaceable or minute samples, or demands the highest level of assay robustness.
Assay Principle: Robust Sandwich ELISA Methodology
This kit operates on the established and reliable sandwich enzyme-linked immunosorbent assay (ELISA) principle. A capture antibody specific to the Rat SERT protein is immobilized on the microplate. During incubation, SERT present in standards and prepared tissue or cell lysates binds to these antibodies. Following a wash step to remove unbound material, a Horseradish Peroxidase (HRP)-conjugated detection antibody is added, forming an antibody-antigen-antibody “sandwich” complex. After a subsequent wash, TMB substrate is added. The HRP enzyme catalyzes a colorimetric reaction, producing a blue product that turns yellow upon addition of the stop solution. The intensity of the yellow color, measured as optical density (OD) at 450 nm, is directly proportional to the concentration of SERT protein present in the original sample.
Commitment to Advancing Your Research
- Premium Quality, Direct Savings: Access specialized neurobiology assay components through our efficient cost-effective rat ELISA Kit model, achieving exceptional value.
- Complimentary Expert Testing Service: Purchase any Yanda Bio ELISA kit and qualify for FREE expert-assisted analysis. Submit your prepared samples, and our specialists will generate and return publication-ready data.
- Publication Recognition Program: Acknowledge the use of our kit with a citation and direct link in your peer-reviewed manuscript to receive a free standard ELISA kit.
- 100% Performance & Satisfaction Guarantee: We stand firmly behind our products. If performance does not meet the specifications detailed in this document, we offer an unconditional replacement or full refund.
- Dedicated 24-Hour Technical Support: Our expert support team is committed to providing a detailed, actionable response to any technical or application inquiry within one business day.
- Custom Assay Development: For novel targets or specialized applications not in our vast catalog, we offer end-to-end custom ELISA kit design, development, and validation services.
Detailed Performance Specifications
- Accuracy & Linearity: Standard curve linear regression demonstrates a correlation coefficient (R) of ≥ 0.9900.
- Precision: Both intra-assay (within-run) and inter-assay (run-to-run) Coefficients of Variation (CV) are < 15%.
- Specificity: The assay shows minimal cross-reactivity with other monoamine transporters (e.g., NET, DAT), ensuring specific SERT detection.
- Sample Compatibility: Validated for rat brain tissue homogenates, intestinal tissue, platelet membranes, and transfected cell lysates.
- Kit Stability & Shelf Life: The complete kit is stable for 6 months from the date of manufacture when stored continuously at 2-8°C.
Ordering & Contact Information
To purchase this Rat Serotonin Transport Protein (SERT) ELISA Kit for sale, or to discuss volume discounts, specific validation protocols, and custom service options, please contact our sales team.
