Kit Highlights & Core Advantages
- Exceptional Sensitivity: Detect E3S/E1S at ultralow levels, with a minimum detection limit of < 1.0 pg/mL.
- Broad Dynamic Range: Achieve precise quantification across a wide 1.0 pg/mL to 400 pg/mL concentration range, suitable for diverse physiological and experimental levels.
- High Specificity: The assay demonstrates minimal cross-reactivity with other endogenous steroids and structurally similar molecules, ensuring reliable and accurate results.
- Superior Reproducibility: Excellent intra-assay (CV < 10%) and inter-assay (CV < 15%) precision guarantees consistency across replicates and batches.
- Unmatched Cost-Efficiency: Access premium-quality hormone assay performance with our direct wholesale pricing, starting below $100 per kit.
- Comprehensive Support Services: We offer tailored custom ELISA development and a complimentary sample testing service to perfectly align with your specific project requirements.
Assay Principle: Advanced Immunoassay Format
This kit utilizes a highly optimized one-step enzyme immunoassay for the specific detection of Human Estrone-3-sulfate. The precise format (competitive or sandwich) is engineered for small molecule detection:
- Immobilization: Microplate wells are pre-coated with a capture system specific to the E3S/E1S molecule or its antibody complex.
- Competitive/Sandwich Binding: Sample (or standard) and an enzyme-conjugated detector (HRP-labeled E3S or anti-E3S antibody) are added simultaneously. The E3S in the sample competes with the labeled conjugate for binding sites, or forms a specific immune complex.
- Signal Development: Following a wash step to remove unbound material, TMB substrate is added. The bound horseradish peroxidase (HRP) catalyzes the conversion of TMB to a blue product.
- Quantification: The reaction is stopped with acid, changing the solution to yellow. The absorbance (OD) at 450 nm is measured inversely or directly proportional to the concentration of Estrone-3-sulfate in the sample.
Detailed Performance Specifications
| Parameter | Specification |
|---|---|
| Accuracy (Linearity) | Correlation coefficient R ≥ 0.9900 |
| Sensitivity | Minimum Detectable Concentration < 1.0 pg/mL |
| Assay Range | 1.0 pg/mL – 400 pg/mL |
| Specificity | No significant cross-reactivity with analogous steroids |
| Precision | Intra-Assay CV < 10%; Inter-Assay CV < 15% |
| Storage | 2-8°C, protect from light and moisture |
| Shelf Life | 4 to 6 months from manufacturing date |
Key Research Applications
- Endocrinology & Reproductive Biology: Study estrogen metabolism, hormonal regulation, and lifecycle-related hormone changes.
- Oncology Research: Investigate the role of E3S as a precursor in estrogen receptor-positive breast cancer and other hormone-dependent cancers.
- Toxicology & Environmental Health: Assess exposure to endocrine-disrupting chemicals (EDCs) by monitoring alterations in estrogen sulfate profiles.
- Drug Development & DMPK: Evaluate the pharmacokinetics and metabolic pathways of steroid-based therapeutics.
Flexible Purchasing & Service Options
Yanda Bio provides adaptable solutions to support your research workflow.
Option 1: Standard Kit Purchase
- Investment: Begin your studies with our standard 48-well kit at a starting price of $99.
- Best For: Laboratories requiring a reliable, validated, and cost-effective off-the-shelf solution for routine E3S/E1S quantification.
Option 2: Custom Assay Development
- Service: Modify the kit’s parameters, including matrix compatibility, calibrator range, or antibody pairs, to suit unique experimental designs.
- Best For: Projects involving novel sample types, non-standard concentration ranges, or specific cross-reactivity profiles.
Option 3: Free Expert Testing Service
- Service: Submit your samples to our application specialists. We will perform the assay and provide you with a comprehensive data report.
- Best For: Initial method verification, feasibility studies, or labs seeking to outsource specific assay runs.
Contact us today for a personalized bulk quotation, to discuss custom development, or to initiate your complimentary sample testing.
FAQ
Q: What is the difference between E3S and E1S?
A: Estrone-3-sulfate (E3S) and Estrone-3-sulfate (E1S) refer to the same molecule. “E1S” denotes it as the sulfate conjugate of Estrone (E1). Our kit detects this specific analyte.
Q: Can this kit be used for urine samples?
A: Yes, the kit is validated for urine, but samples typically require appropriate dilution. Please refer to the protocol or contact us for specific guidance on sample preparation.
Q: How is such affordable pricing achieved?
A: Through optimized production, efficient sourcing, and a direct-to-researcher model, we eliminate intermediaries to offer high-performance kits at a disruptive price point.
Q: What does the free testing service include?
A: You send us your prepared samples. We run the full assay using this kit in our QC lab and return a detailed analytical report with raw data and calculated concentrations.
Your Partner in Hormone Analysis
Yanda Bio is dedicated to empowering scientific discovery with robust and accessible bioanalytical tools. Our Human Estrone-3-sulfate (E3S/E1S) ELISA Kit delivering uncompromised sensitivity, specificity, and value. Backed by our flexible custom services and expert support, we provide a complete solution to advance your research .
Elevate your hormone research with precision and affordability. Order now or inquire about our specialized services.
