Product Overview: Why Measure Dihydrotestosterone (DHT)?
Dihydrotestosterone (DHT) is the most potent naturally occurring androgen, synthesized from testosterone by the enzyme 5α‑reductase. It plays a critical role in male sexual development, prostate growth, hair follicle biology, and endocrine signaling. Dysregulation of DHT levels is implicated in benign prostatic hyperplasia (BPH), prostate cancer, androgenetic alopecia (male pattern baldness), polycystic ovary syndrome (PCOS), and various endocrine disorders. Accurate quantification of Human DHT is essential for:
- Endocrinology and reproductive biology research
- Prostate disease and androgen‑dependent conditions
- Dermatology (hair loss, hirsutism) studies
- Pharmacological evaluation of 5α‑reductase inhibitors
This kit uses a Sandwich ELISA Kit format (not competitive) for robust, high‑throughput measurement of DHT with exceptional specificity and minimal cross‑reactivity.
Why Choose Yanda Bio for Your DHT ELISA Needs?
| EEAT Factor | Our Commitment |
|---|---|
| Experience | 6,000+ factors developed; 60+ university collaborations; years of expertise in steroid hormone assays. |
| Expertise | Proprietary antibody pairs validated for human DHT; unique sandwich ELISA design ensures high specificity and minimal cross‑reactivity with testosterone, androstenedione, and other steroids. |
| Authoritativeness | Kits used in academic publications; ISO‑aligned manufacturing; batch‑to‑batch consistency. |
| Trustworthiness | Rapid custom development (3–7 days); unconditional quality guarantee; free shipping on 3+ kits. |
Superior Quality Metrics
- Intra‑assay CV < 5 % — excellent within‑run precision
- Inter‑assay CV < 8 % — outstanding run‑to‑run consistency
- High specificity — cross‑reactivity with testosterone < 0.5 %, with other androgens < 0.1 %
Kit Components: What’s Inside
| Component | 96‑Well Configuration | 48‑Well Configuration |
|---|---|---|
| Microplate (Pre‑coated) | 8 wells × 12 strips | 8 wells × 6 strips |
| Standards | 0.3 mL × 6 tubes | 0.3 mL × 6 tubes |
| Sample Diluent | 6 mL | 3 mL |
| HRP‑Conjugated Detection Antibody | 6 mL | 3 mL |
| 20× Wash Buffer | 25 mL | 15 mL |
| Substrate A | 6 mL | 3 mL |
| Substrate B | 6 mL | 3 mL |
| Stop Solution | 6 mL | 3 mL |
| Plate Sealer | 2 sheets | 2 sheets |
| Manual | 1 copy | 1 copy |
Note: This is a Sandwich ELISA Kit configuration – not competitive – ensuring direct proportional signal for easier data interpretation.
Assay Principle: Sandwich ELISA
This kit employs a solid‑phase sandwich enzyme‑linked immunosorbent assay (a true Sandwich ELISA Kit). The microplate is pre‑coated with a monoclonal antibody specific for Human Dihydrotestosterone (DHT). Standards and samples are added, allowing DHT to bind to the immobilised antibody. After washing, a Horseradish Peroxidase (HRP)‑conjugated detection antibody is added, forming an antibody‑antigen‑antibody “sandwich”. Following a second wash, TMB substrate is added. The HRP enzyme catalyses a blue colour that turns yellow upon acidification. The optical density (OD) at 450 nm is directly proportional to the DHT concentration in the sample.
Sample Dilution Note: Samples are pre‑diluted 5‑fold during the procedure; multiply results by 5 to obtain actual concentrations.
Sample Collection & Preparation
| Sample Type | Collection & Processing |
|---|---|
| Serum | Use pyrogen/endotoxin‑free tubes. Avoid cell stimulation. Centrifuge at 3000 rpm for 10 minutes. |
| Plasma | Use EDTA, citrate or heparin anticoagulant. Centrifuge at 3000 rpm for 30 minutes. |
| Cell Supernatant | Centrifuge at 3000 rpm for 10 minutes to remove particles. |
| Tissue Homogenate | Homogenise tissue in PBS (1:9 w/v), centrifuge at 5000×g for 10 minutes at 2–8 °C. Collect supernatant. |
| Storage | Aliquot and store at –20 °C (≤ 1 month) or –80 °C (≤ 2 months). Avoid repeated freeze‑thaw cycles. |
Materials Required But Not Provided
- Microplate reader (450 nm)
- Precision pipettes and tips (0.5–10 μL, 2–20 μL, 20–200 μL, 200–1000 μL)
- 37 °C incubator or water bath
- Distilled or deionised water
Important Notes for Assay Procedure
- Store the kit at 2–8 °C. Bring all reagents to room temperature for 20 minutes before use. Concentrated wash buffer may crystallise; dissolve crystals in a warm water bath.
- Return unused strips to the resealable bag with desiccant and store at 2–8 °C. Use promptly.
- The S0 standard (zero concentration) is the negative control/blank. Multiply final results by 5 (dilution factor).
- Strictly adhere to incubation times, volumes and sequence.
- Mix all liquid components thoroughly before use.
Reagent Preparation
20× Wash Buffer Dilution: Dilute 1 part 20× wash buffer with 19 parts distilled water to obtain 1× working wash buffer.
Washing Method
Manual: Aspirate liquid, fill each well with wash buffer, stand for 1 min, aspirate, and tap dry on absorbent paper. Repeat 5 times.
Automatic washer: Inject 350 μL per well, soak for 1 min, wash 5 times.
Assay Procedure
- Remove required strips after 20 min equilibration. Reseal unused strips and return to 4 °C.
- Standard wells: add 50 μL of each standard concentration.
- Sample wells: add 10 μL sample + 40 μL sample diluent. Blank wells: leave empty.
- Add 100 μL HRP‑conjugated detection antibody to all wells except blanks. Seal and incubate at 37 °C for 60 min.
- Aspirate, wash 5 times (manual or automatic).
- Add 50 μL Substrate A and 50 μL Substrate B to each well. Incubate at 37 °C in the dark for 15 min.
- Add 50 μL stop solution. Measure OD at 450 nm within 15 min.
Data Analysis
Plot standard curve: OD values (y‑axis) vs. standard concentrations (x‑axis) in Excel or curve‑fitting software (linear regression, R ≥ 0.9900). Calculate sample concentrations from the curve, then multiply by the dilution factor (5×).
Kit Performance Specifications
| Parameter | Performance |
|---|---|
| Accuracy | Standard curve linear regression coefficient (R) ≥ 0.9900 |
| Sensitivity | Minimum detectable concentration < 0.02 ng/mL |
| Detection Range | 0.05 – 10 ng/mL |
| Specificity | No significant cross‑reactivity with testosterone, androstenedione, estradiol, progesterone, or cortisol |
| Intra‑assay CV | < 5 % |
| Inter‑assay CV | < 8 % |
| Storage | 2–8 °C, protect from light and moisture |
| Shelf Life | 6 months |
Best Practices for Recording ELISA Experiment Information
Accurate record‑keeping is essential for reproducibility. Document the following for each assay:
- Date of experiment and operator name
- Experiment objective and detection target (Human DHT)
- Sample information: source, processing steps, storage conditions, dilution factors
- Kit lot number and expiration date
- Standard curve data: concentrations and corresponding OD values (raw and blank‑subtracted)
- Sample OD values and calculated concentrations
- Any deviations from the standard protocol
- Observations: unusual colour development, precipitate, bubbles, etc.
Keeping a clear, traceable lab notebook facilitates troubleshooting, data validation, and learning from both successful and failed experiments.
Related Products & Internal Link
For researchers who require a competitive ELISA format for DHT quantification, we offer an alternative assay configuration. Please visit our product page for the Competitive Dihydrotestosterone (DHT) ELISA Kit (link to be added by user).
Other recommended androgens and steroid hormone kits available from Yanda Bio:
- Human Testosterone ELISA Kit (Sandwich format)
- Human Androstenedione ELISA Kit
- Human Cortisol ELISA Kit
These kits are fully compatible with the same sample types and offer the same high precision and reliability.
Pricing & Shipping Information
| Quantity | Price per Kit |
|---|---|
| 1–2 kits | Standard retail – contact us for current pricing |
| 3 or more kits | Wholesale price – cost‑effective for multi‑kit orders |
Free shipping on all orders of three or more ELISA kits. All products are tax‑included and delivered free to your doorstep.
Free sample testing service is available – contact our sales team for details.
Why Choose Yanda Bio?
- Manufacturer direct – over 6,000 detectable targets, 60+ university partners
- Rapid custom development – 3–7 days turnaround for new targets or matrices
- Strict quality control – intra‑assay CV < 5 %, inter‑assay CV < 8 %
- Cost‑effective – premium performance at 40–60 % lower cost than leading international brands
- Free shipping on 3+ kits
- Free technical support from sample collection to data analysis
Disclaimer
Strict adherence to the protocol is required. Yanda Bio is not responsible for results obtained from deviations. This product is for research use only; not for clinical diagnosis or human use. The user assumes all responsibility for any consequences arising from misuse.
Ordering & Contact Information
To order this Human Dihydrotestosterone (DHT) ELISA Kit – a high‑performance Sandwich ELISA Kit for androgen research – or to inquire about volume discounts, custom development, and format selection, please contact our sales team.
