Key Product Advantages & Features
- Ultra-High Sensitivity: Detect 1,3-β-DG at concentrations as low as < 0.1 pg/mL, enabling early and accurate biomarker identification.
- Excellent Specificity: Minimal cross-reactivity ensures accurate quantification without interference from structurally similar molecules.
- Rapid & Streamlined Protocol: The optimized one-step assay reduces total procedure time, increasing lab throughput and efficiency.
- Ready-to-Use Components: Includes all necessary reagents, pre-coated plates, and detailed protocols for immediate use.
- Cost-Effective Solution: Achieve premium performance without premium cost. Our Human ELISA test Kit is available for purchase online with bulk pricing starting below $100.
- Comprehensive Support: We offer custom ELISA kit development and a complimentary sample testing service to meet your unique research requirements.
Assay Principle: One-Step Sandwich ELISA
This kit utilizes a sensitive one-step sandwich enzyme-linked immunosorbent assay (ELISA):
- Capture: Microplate wells are pre-coated with an antibody specific to Human β-(1,3)-D-glucan (1,3-β-DG).
- Simultaneous Incubation: Samples or standards are added simultaneously with the Horseradish Peroxidase (HRP)-conjugated detection antibody. The target 1,3-β-DG is “sandwiched” between the two antibodies in a single incubation step.
- Signal Development: After washing to remove unbound material, a TMB substrate is added. The HRP enzyme catalyzes the conversion of TMB to a blue product.
- Quantification: The reaction is stopped with acid, changing the color to yellow. The absorbance (OD) at 450 nm is measured. The color intensity is directly proportional to the concentration of 1,3-β-DG in the sample.
Complete Kit Specifications & Contents
Performance Characteristics
| Parameter | Specification |
|---|---|
| Accuracy (Linearity) | R-value ≥ 0.9900 |
| Sensitivity | < 0.1 pg/mL |
| Detection Range | 0.1 – 12 pg/mL |
| Specificity | No significant cross-reactivity observed |
| Precision (CV) | Intra & Inter-assay CV < 15% |
| Storage | 2-8°C, protect from light & moisture |
| Shelf Life | 6 months |
Standard Curve Concentrations
S0 to S5: 0, 0.75, 1.5, 3, 6, 12 pg/mL
Kit Components (96T / 48T Configurations)
- Pre-coated Microplate (8 strips / 4 strips)
- Standards (0.3mL x 6 tubes)
- Sample Diluent (6mL / 3mL)
- Detection Antibody-HRP (10mL / 5mL)
- 20x Wash Buffer (25mL / 15mL)
- Substrate A & B (6mL each / 3mL each)
- Stop Solution (6mL / 3mL)
- Plate Sealers, Manual, Zipper Bag
Sample Collection & Preparation Guidelines
- Serum/Plasma: Collect using aseptic, pyrogen-free tubes. Centrifuge at 3000xg for 10-30 minutes to separate clear supernatant.
- Cell Culture Supernate: Centrifuge at 3000xg for 10 minutes to remove particulates.
- Storage: For delayed analysis, aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles. Thaw completely at room temperature and mix gently before use.
Essential Workflow Overview
- Reagent & Plate Prep: Bring all components to room temperature (20 min). Dilute the 20x Wash Buffer 1:20 with distilled water.
- Sample Dilution: Dilute samples 1:5 with Sample Diluent (10μL sample + 40μL diluent).
- Assay Incubation: Add standards or diluted samples to wells. Add HRP-conjugated Detection Antibody to all wells except blank. Incubate at 37°C for 60 minutes.
- Washing: Wash the plate 5 times with diluted wash buffer.
- Color Development: Add Substrate A and B (50μL each). Incubate at 37°C for 15 minutes in the dark.
- Stop & Read: Add Stop Solution (50μL). Measure absorbance at 450 nm within 15 minutes.
Note: The S0 standard serves as the zero/blank control. Multiply sample results by the dilution factor (5) to obtain the actual concentration.
Required Materials (Not Included)
- Microplate Reader (450 nm)
- Precision Pipettes and Tips (e.g., 10μL, 200μL, 1000μL)
- 37°C Incubator
- Deionized Water
Primary Research Applications
- Invasive Fungal Infection (IFI) Research: Quantitative detection of 1,3-β-DG as a serum biomarker for candidiasis, aspergillosis, etc.
- Immunology Studies: Investigate host-pathogen interactions and immune responses to fungal elements.
- Therapeutic Drug Monitoring: Assess treatment efficacy in antifungal therapy.
- Biomarker Validation: Support diagnostic assay development and validation studies.
Ordering & Support Services
Option 1: Purchase Standard Kit
- Pricing: Contact for quote. Competitive bulk pricing under $100 for high-volume orders.
- Best For: Standard research applications requiring reliable, off-the-shelf performance.
Option 2: Custom Assay Development
- Service: Tailor the kit’s parameters, such as range, sensitivity, or sample type compatibility, for specialized projects.
Option 3: Free Sample Testing Service
- Service: Submit your samples. Our experts will perform the assay and provide a detailed report. Ideal for protocol validation.
Ready to enhance your fungal biomarker research? Contact us to buy this Human ELISA test Kit online, request a custom quote, or schedule your free sample test.
Frequently Asked Questions (FAQ)
Q: What makes this a “QuickDetect” kit?
A: The QuickDetect name highlights our optimized one-step protocol, which significantly reduces total hands-on and incubation time compared to traditional two-step ELISA formats, delivering faster results.
Q: Why is the sensitivity of <0.1 pg/mL important?
A: Extremely low levels of 1,3-β-D-glucan can be clinically significant. This high sensitivity allows for the earliest possible detection, which is critical for timely intervention in infection models.
Q: How do you maintain such low prices?
A: Our direct-to-researcher model and optimized manufacturing processes eliminate unnecessary markups, allowing us to offer high-quality Human ELISA test Kits for sale online at wholesale prices consistently under $100.
Q: Is this kit for diagnostic use?
A: No. This product is sold for Research Use Only (RUO). It is not intended for use in diagnostic procedures.
Disclaimer
- This kit is intended for research purposes only. It is not validated or approved for use in clinical, diagnostic, or therapeutic procedures involving humans.
- Users must adhere strictly to the provided instructions. The manufacturer is not liable for consequences arising from the misuse or modification of the prescribed protocol.
