Rat 3-Indolepropionic Acid (3-IPA) ELISA Kit: Quantifying a Key Microbial Metabolite
Yanda Bio’s Rat 3-Indolepropionic Acid (3-IPA) ELISA Kit provides a highly sensitive and robust tool for the specific quantification of 3-IPA in rat serum, plasma, tissue homogenates (e.g., brain, liver), and fecal samples. As a microbially derived tryptophan metabolite, 3-IPA is a critical biomarker at the intersection of gut health, metabolism, and neurobiology. It exhibits antioxidant and anti-inflammatory properties, making it essential for research in metabolic syndrome, neurodegenerative diseases, gut barrier function, and the gut-brain axis using rat models.
As a leading factory-direct manufacturer with advanced R&D facilities in China, we deliver premium-quality assays with unmatched value. This ELISA test kit made in China is offered at a retail price of $120, with significant bulk pricing under $100. Our extensive capabilities are demonstrated by a portfolio of over 6,000 detection targets across key disciplines, supported by proven collaborations with more than 70 universities worldwide.
Select Your Precision Level: Two Optimized Assay Tiers
To accommodate diverse sensitivity requirements and research budgets, we offer two validated kit configurations. Purchasing either tier grants you access to our complimentary expert sample testing service.
| Feature | Tier 1: Standard 3-IPA ELISA Kit | Tier 2: High-Sensitivity 3-IPA ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with a proprietary matrix-interference diluent. | Biotinylated recombinant monoclonal antibody (>95% pure) combined with a Streptavidin-HRP conjugate (≥500 U/mg). |
| Key Advantage | Exceptional Value & Reliability. Employs a single-step incubation protocol and a specialized diluent engineered to effectively suppress background noise from common interferents like hemoglobin and lipids, ensuring stable and reproducible results in complex biological samples. | Superior Sensitivity & Specificity. The advanced biotin-streptavidin signal amplification system directly neutralizes matrix effects, providing an outstanding signal-to-noise ratio for detecting ultra-low levels of 3-IPA in precious or challenging sample types. |
| Best Suited For | Routine analysis in dietary, metabolic, or microbiome studies where sample volumes are sufficient and 3-IPA concentrations are within a standard range. | High-precision research requiring the lowest possible detection limits (e.g., cerebral spinal fluid analysis), work with limited sample volumes, or studies involving highly complex matrices. |
Complimentary Expert Testing Service: Reliable Data, Accelerated
Streamline your research workflow by utilizing our professional laboratory service, included with your purchase.
- Guaranteed Rapid Start: We initiate your assay within 24 hours of sample receipt.
- Fast, Comprehensive Reporting: Receive a complete data package in approximately 5 business days, including raw OD values, calculated concentration tables, and a standard curve graph.
- Effortless Method Validation: An ideal solution to confirm assay performance with your specific rat sample matrices before establishing an in-house protocol.
Our Commitment: Building Trust Through Action
We establish lasting partnerships with researchers through tangible commitments:
- Publication Recognition: Cite this Rat 3-IPA ELISA Kit and link to our product page in your publication, and we will send you a complimentary standard ELISA kit (up to $120 value) as our thanks.
- 100% Satisfaction Guarantee: We confidently stand behind our products. Should any kit not meet its published performance specifications, we offer an unconditional replacement or refund.
- Responsive Support Pledge: Our dedicated team commits to providing a clear and actionable resolution path within 24 hours of any inquiry.
- Integrity in Research: We handle every sample with meticulous care, adhering to rigorous standards to protect the validity and value of your scientific work.
Technical Specifications & Assay Principle
Detection Principle: This kit utilizes a validated one-step sandwich ELISA. Rat 3-IPA in the sample is captured between an immobilized antibody and an HRP-conjugated detection antibody during a single incubation. The subsequent enzymatic reaction with TMB substrate produces a colorimetric signal measured at 450 nm, which is directly proportional to the concentration of 3-IPA.
Performance Characteristics:
- Sensitivity: < 1.0 pg/mL.
- Detection Range: 1.0 – 160 pg/mL.
- Accuracy (Linearity): Correlation Coefficient R ≥ 0.9900.
- Precision: Intra- and Inter-assay Coefficient of Variation (CV) < 15%.
- Specificity: High specificity for rat 3-IPA, with minimal cross-reactivity.
- Storage & Stability: Store at 2-8°C, protected from light and moisture; stable for 6 months.
Sample Preparation: For fecal samples, appropriate metabolite extraction is required. Clarify all liquid samples (serum, plasma) by centrifugation (e.g., 3000×g for 10-15 minutes). Aliquot and store all samples at -20°C, minimizing freeze-thaw cycles.
Streamlined Assay Workflow
- Addition: Pipette 50 µL of standards or appropriately prepared/diluted samples into the pre-coated wells.
- Incubation: Add 100 µL of the HRP-conjugated Detection Antibody. Incubate at 37°C for 60 minutes.
- Wash: Aspirate and wash the microplate 5 times thoroughly with the diluted wash buffer.
- Color Development: Add 50 µL each of Substrate A and B. Incubate at 37°C for 15 minutes, protected from light.
- Measurement: Add 50 µL Stop Solution. Measure the absorbance at 450 nm within 15 minutes.
Pricing & Service Options
| Option | Description | Details |
|---|---|---|
| Tier 1: Standard Kit | Cost-effective, reliable solution for in-laboratory use. | Retail: $120. Bulk: < $100 (quote required). |
| Tier 2: High-Sensitivity Kit | Premium performance for maximum sensitivity requirements. | Contact for competitive manufacturer price. |
| Expert Testing Service | Full-service assay execution and detailed data reporting. | ~5-day turnaround. Free with kit purchase. |
| Custom ELISA Development | Tailored assay solutions from our extensive portfolio of 6,000+ targets. | Project-based quotation available. |
To order your Rat 3-IPA ELISA Kit or to explore our vast assay catalog, contact Yanda Bio, your trusted direct manufacturer in China.
Research Applications & Frequently Asked Questions
Primary Applications (Research Use Only): Gut-brain axis research, neuroinflammatory and neurodegenerative disease models (e.g., Alzheimer’s, Parkinson’s), metabolic disorder studies (obesity, diabetes), gut microbiota research, and investigations into antioxidant and anti-inflammatory mechanisms.
Q: Why is 3-IPA an important research target?
A: 3-IPA is a gut microbiota-derived metabolite with demonstrated neuroprotective, antioxidant, and anti-inflammatory properties. Its levels are increasingly linked to metabolic health, gut barrier integrity, and resilience against neurodegenerative pathologies, making it a valuable biomarker in preclinical rodent models.
Q: How do I claim the free kit for a publication?
A: Upon publication of your peer-reviewed article that cites the “Rat 3-Indolepropionic Acid (3-IPA) ELISA Kit (Yanda Bio)” and includes a direct link to our product page, send us the citation details to claim your complimentary reward.
Q: Can this kit be used for mouse samples?
A: This kit is specifically validated for rat 3-IPA. For accurate quantification in mouse samples, please inquire about our species-specific or custom ELISA development services.
Q: What are the advantages of sourcing from a direct manufacturer in China?
A: Partnering with Yanda Bio provides direct access to competitive manufacturer pricing, eliminates intermediary markups, ensures seamless communication with our R&D and QC teams, and guarantees full transparency over our stringent manufacturing and quality control processes.
Manufacturer’s Note
This product is developed and sold for Research Use Only (RUO). It is not intended for diagnostic, therapeutic, or clinical procedures. The researcher is responsible for validating the kit’s suitability for their specific experimental application.
