Mouse Myostatin (MSTN) ELISA Kit for Skeletal Muscle & Metabolism Research
Yanda Bio’s Mouse Myostatin (MSTN) ELISA Kit provides a specific and highly sensitive tool for the quantitative detection of Myostatin (also known as GDF-8) in mouse serum, plasma, cell culture supernatants, and tissue lysates (especially skeletal muscle). As a critical negative regulator of skeletal muscle mass, MSTN is a primary research target in studies of muscle wasting (cachexia), sarcopenia, metabolic disorders, and performance enhancement. This reliable ELISA test kit delivers precise data to advance your research in physiology, sports science, and metabolic disease.
As a recognized bio-company and Elisa test kit manufacturer in China, we guarantee both quality and value. This kit is available at a competitive retail price, with bulk orders priced under $100. Yanda Bio offers an extensive portfolio of over 6,000 detection targets covering immunology, oncology, neuroscience, metabolism, and infectious diseases, serving researchers worldwide. Our quality is endorsed by long-term collaborations with more than 70 universities.
Two Tiers for Tailored Sensitivity and Value
We offer two optimized kit formats to suit different experimental precision and budget requirements. Purchasing either tier includes access to our complimentary expert testing service.
| Feature | Tier 1: Standard Mouse MSTN ELISA Kit | Tier 2: High-Sensitivity Mouse MSTN ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA featuring a specialized matrix-suppression diluent. | Biotinylated recombinant monoclonal antibody (>95% purity) coupled with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). |
| Primary Advantage | Excellent Cost-Effectiveness & Reliability. Uses a simplified single incubation step. The proprietary diluent actively reduces background interference from hemoglobin and lipids, ensuring stable and consistent performance for routine analyses. | Maximum Sensitivity & Interference Resistance. The advanced biotin-streptavidin amplification system directly neutralizes matrix effects, delivering an exceptional signal-to-noise ratio. This is crucial for detecting very low MSTN levels or analyzing complex sample types with high precision. |
| Recommended Use | Ideal for studies where sample MSTN concentrations are expected within a standard detectable range. | Essential for high-sensitivity applications, such as detecting subtle changes in expression, working with limited sample volumes, or analyzing challenging biological matrices. |
Included Expert Testing Service: Fast, Authoritative Results
Save time and ensure optimal data quality by utilizing our professional in-house laboratory service, included with your purchase.
- Rapid Initiation: We start processing your samples within 24 hours of receipt.
- Comprehensive & Timely Reporting: Receive a detailed data package in approximately 5 business days, containing raw OD values, calculated concentration tables, and a standard curve graph.
- Hassle-Free Validation: This service is perfect for validating the assay’s performance with your specific mouse models before committing to large-scale in-house experiments.
Our Unwavering Commitment to Your Scientific Success
We build trust through transparent and actionable guarantees:
- Publication Recognition: Cite this Mouse MSTN ELISA Kit with a link to our product page in your publication, and we will reward you with a complimentary standard ELISA kit (up to $120 value).
- 100% Performance Guarantee: We stand firmly behind our products. If any kit does not meet its stated specifications, we offer an unconditional replacement or refund.
- 24-Hour Support Pledge: Our dedicated support team is committed to providing a clear resolution path within 24 hours of any inquiry.
- Your Research Partner: We understand the critical importance of every sample and experiment, and we are committed to supporting your work with integrity and professional rigor.
Intended Use, Principle & Key Specifications
Intended Use: For Research Use Only (RUO). Not for diagnostic or therapeutic procedures.
Test Principle: The kit employs a sandwich ELISA. Mouse MSTN is captured between an immobilized antibody and an enzyme-linked detection antibody. After washing, a TMB substrate produces a blue color, which turns yellow upon stopping. The color intensity, measured at 450 nm, is proportional to the MSTN concentration in the sample.
Key Performance Data:
- Assay Range: 1.25 – 40 ng/mL.
- Sensitivity: Typically < 0.1 ng/mL.
- Precision: Intra-assay CV < 10%; Inter-assay CV < 15%.
- Specificity: Recognizes both recombinant and natural mouse MSTN. No significant cross-reactivity observed.
- Storage: Store at 2-8°C for frequent use; stable for six months at -20°C.
Sample Collection & Storage Guidelines
- Serum/Plasma: Collect using appropriate tubes. Centrifuge at 1000×g (serum: after clotting; plasma: within 30 mins). Aliquot and store at -20°C or lower. Avoid repeated freeze-thaw cycles.
- Tissue Homogenates: Rinse tissues with ice-cold PBS, homogenize, sonicate if needed, and centrifuge at 5000×g to collect supernatant.
- Cell Culture Supernates & Other Fluids: Centrifuge at 1000×g for 20 minutes to remove particulates before aliquoting and freezing.
- General Note: Ensure samples are adequately centrifuged and free from hemolysis or granules.
Essential Assay Workflow Summary
- Add 50 µL of standards or samples to wells (blank well receives nothing).
- Add 100 µL of Enzyme Conjugate to all wells except blank. Incubate at 37°C for 60 min.
- Wash the microplate 4 times thoroughly.
- Add 50 µL each of Substrate A and B. Incubate at 37°C for 15 min in the dark.
- Add 50 µL Stop Solution. Read the Optical Density (O.D.) at 450 nm within 15 minutes.
Calculation: Generate a standard curve by plotting the average O.D. (450nm) for each standard against its concentration. Determine sample concentrations by interpolating from the curve. Remember to subtract the blank well’s mean O.D. value.
Ordering Information & Value-Added Services
| Option | Description | Details |
|---|---|---|
| Tier 1: Standard Kit Purchase | Cost-effective, reliable solution for in-laboratory use. | Retail: $120. Bulk: < $100 (contact for quote). |
| Tier 2: High-Sensitivity Kit Purchase | Premium-grade kit for maximum sensitivity requirements. | Contact for competitive manufacturer price. |
| Expert Testing Service | Full-service assay execution and detailed report. | ~5-day turnaround. Free with kit purchase. |
| Custom ELISA Development | Tailored solutions from our portfolio of 6,000+ targets. | Project-based quotation available. |
To order your Mouse MSTN ELISA Kit or to inquire about our vast assay catalog, contact Yanda Bio today.
Research Applications & Frequently Asked Questions
Primary Applications (RUO): Research on muscle atrophy (cachexia, sarcopenia), muscular dystrophy, obesity and metabolic syndrome, exercise physiology, and therapeutic development for muscle-related disorders.
Q: Why is Myostatin (MSTN) an important research target?
A: MSTN is a master negative regulator of skeletal muscle growth. Inhibiting its function can lead to increased muscle mass, making it a key target for understanding and treating conditions involving muscle wasting and for enhancing performance.
Q: How do I claim the free kit for a publication?
A: Upon publication of your article that cites the “Mouse Myostatin (MSTN) ELISA Kit (Yanda Bio)” and includes a direct link to this product page, send us the citation details. We will promptly ship your complimentary reward.
Q: What are the critical precautions when using this kit?
A: Key precautions include: allowing all reagents to reach room temperature before use; never mixing reagents from different kit lots; using fresh pipette tips to avoid contamination; handling all samples as potentially hazardous; and ensuring the Substrate Solution is colorless before use.
Q: Why choose Yanda Bio as your supplier from China?
A: As a well-known Chinese biotech company and direct manufacturer, we provide authentic manufacturer pricing, stringent in-house quality control, direct access to technical expertise, and a vast portfolio of over 6,000 assays, ensuring you get the best value and support.
Manufacturer’s Note
This product is for Research Use Only (RUO). It is not intended for use in diagnostic, therapeutic, clinical, or animal clinical procedures. All samples should be considered potentially infectious and must be handled and disposed of following good laboratory practices and appropriate safety regulations.
