Mouse Coagulation Factor VII (FVII) & Factor V (FV) ELISA Kits for Hemostasis and Thrombosis Research
Yanda Bio presents two highly specific and sensitive ELISA kits designed for the precise quantification of critical coagulation cascade proteins in mouse models: the Mouse Coagulation Factor VII (FVII) ELISA Kit and the Mouse Coagulation Factor V (FV) ELISA Kit. These assays enable accurate measurement of their respective analytes in mouse plasma, serum, and other relevant biological matrices. As essential components of the coagulation pathway, FVII (initiator of the extrinsic pathway) and FV (a key cofactor in the common pathway) are vital research targets in studies of bleeding disorders, thrombosis, disseminated intravascular coagulation (DIC), and the effects of anticoagulant therapies.
As a factory-direct manufacturer with dedicated research laboratories in China, we offer premium-quality reagents at competitive prices. Each kit is available at a competitive retail price, with attractive bulk pricing for high-throughput studies. Yanda Bio offers an extensive portfolio of over 6,000 detection targets across immunology, oncology, neuroscience, and more, and our expertise is validated by collaborative partnerships with over 70 universities worldwide.
Product Comparison: Choose Your Specific Target
Our kits are optimized for distinct but equally critical components of the coagulation system. Below is a comparison to guide your selection.
| Parameter | Mouse Coagulation Factor VII (FVII) ELISA Kit | Mouse Coagulation Factor V (FV) ELISA Kit |
|---|---|---|
| Target Pathway | Extrinsic Pathway Initiator | Common Pathway Cofactor |
| Primary Biological Role | Binds Tissue Factor (TF) to initiate coagulation in response to vascular injury. | Serves as a crucial non-enzymatic cofactor for Factor Xa in prothrombin activation. |
| Typical Research Applications | Studying TF-FVIIa complex biology, sepsis, atherosclerosis, and therapeutic monitoring of FVII-targeting agents. | Investigating bleeding diatheses (e.g., Parahemophilia model), thrombophilia, DIC, and APC resistance. |
| Detection Principle | One-Step Sandwich ELISA with optimized protocols. | One-Step Sandwich ELISA with optimized protocols. |
| Performance Tier | Available in Standard and High-Sensitivity (Biotin-Streptavidin) formats. | Available in Standard and High-Sensitivity (Biotin-Streptavidin) formats. |
| Assay Range (Example) | 0.1 – 50 ng/mL* | 1.0 – 480 pg/mL |
| Sensitivity (Example) | < 0.05 ng/mL* | < 1.0 pg/mL |
| Common Sample Types | Citrate or EDTA plasma (recommended), serum. | Citrate or EDTA plasma (recommended), serum. |
| *Exact specifications for FVII are batch-specific; please contact us for the latest datasheet. |
Key Features & Benefits Common to Both Kits
- Target-Specific Accuracy: Each kit employs highly specific antibodies, ensuring precise measurement of its intended coagulation factor with minimal cross-reactivity.
- Dual-Format Flexibility: Both kits are available in two performance tiers to match your needs:
- Standard Tier: Features an optimized one-step incubation and a specialized sample diluent to reduce background interference from plasma components.
- High-Sensitivity Tier: Utilizes a >95% pure biotinylated recombinant monoclonal antibody and a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This system provides superior signal amplification, directly mitigates matrix effects, and is ideal for detecting very low analyte levels.
- Free Expert Testing Service Included: Purchasing any kit grants access to our professional service. We initiate testing within 24 hours of sample receipt and deliver a complete data package (raw data, concentration tables, standard curve) in ~5 business days.
- Exceptional Value: Benefit from direct manufacturer pricing, with bulk order discounts available.
Our Commitment to Research Integrity
- Publication Reward: Cite either of these ELISA Kits in your publication with a link to our website, and receive a complimentary standard ELISA kit (up to $120 value).
- 100% Satisfaction Guarantee: We offer an unconditional replacement or refund if a product does not meet its performance specifications.
- Dedicated 24-Hour Support: Our team provides a proposed resolution within one business day of any inquiry.
- Guardians of Your Research: We handle every sample with the care and precision your important work deserves.
Assay Principle & General Specifications
Detection Principle (for both): The kits utilize a one-step sandwich ELISA. The target coagulation factor (FVII or FV) in the sample is captured between a pre-coated specific antibody and an HRP-conjugated detection antibody. After washing, a TMB substrate reaction produces a color change measured at 450 nm, with intensity proportional to the analyte concentration.
General Performance (Example based on provided FV data):
- Accuracy: Correlation Coefficient R ≥ 0.9900.
- Precision: Intra- and Inter-assay CV < 15%.
- Specificity: High specificity with no significant cross-reactivity.
- Storage: 2-8°C, protected from light; 6-month shelf life.
Sample Handling: For optimal results, use citrate or EDTA plasma collected and processed according to standard coagulation assay protocols to avoid activation. Centrifuge samples thoroughly (e.g., 3000×g). Aliquot and store at -20°C or lower, avoiding repeated freeze-thaw cycles. A 1:5 sample dilution is typically required.
Ordering Information & Services
| Option | Description | Details |
|---|---|---|
| Kit Purchase (FVII or FV) | Choose your target and performance tier (Standard or High-Sensitivity). | Contact for manufacturer price & bulk quotes (under $100 range). |
| Expert Testing Service | We run the assay for you and provide a complete report. | ~5-day turnaround. Free with kit purchase. |
| Custom ELISA Development | Tailored solutions for novel coagulation targets. | Project-based quotation. |
To order your Mouse Coagulation Factor ELISA Kit or request specific performance data, contact Yanda Bio, your trusted supplier in China.
Research Applications & Frequently Asked Questions (FAQ)
Primary Applications (RUO): Basic hemostasis research, creating and characterizing mouse models of bleeding/thrombotic disorders, evaluating the efficacy and mechanism of novel anticoagulant/hemostatic drugs, and studying conditions like sepsis, cancer-associated thrombosis, and liver disease.
Q: Which sample type is best for measuring coagulation factors?
A: Citrate or EDTA-anticoagulated plasma is strongly recommended. Serum contains activated and/or consumed coagulation factors, which may not reflect the true circulating zymogen levels.
Q: How do I choose between the Standard and High-Sensitivity tier?
A: The Standard tier is excellent for most applications where factor levels are within normal or elevated ranges. The High-Sensitivity tier is crucial for detecting very low levels, such as in deficiency models, or when sample volume is extremely limited.
Q: How do I claim the free kit for a publication?
A: Once your peer-reviewed article citing the specific “Mouse FVII/FV ELISA Kit (Yanda Bio)” with a link to our site is published, send us the citation. We will fulfill your reward.
Q: Why source from a manufacturer in China?
A: Sourcing directly from Yanda Bio in China ensures you receive the authentic manufacturer price, benefit from in-house R&D and QC, and gain direct access to technical support for these specialized assays.
Manufacturer’s Note
These products are for Research Use Only (RUO). They are not intended for diagnostic, therapeutic, clinical, or animal clinical procedures. Proper sample handling and assay technique are essential for accurate results.
