Mouse DLAT & PDC-E2 Quantification ELISA Kits for Metabolism & Autoimmunity Research
Yanda Bio offers two specialized ELISA kits for pivotal research targets in mouse models: the Mouse Dihydrolipoamide S-Acetyltransferase (DLAT) ELISA Kit and the Mouse Pyruvate Dehydrogenase Complex E2 (PDC-E2) ELISA Kit. These assays enable the specific and quantitative measurement of DLAT and PDC-E2 proteins in tissue lysates (liver, heart), cell extracts, and other biological samples. DLAT is a core component of the pyruvate dehydrogenase complex (PDC), with emerging significance in cancer metabolism and cuproptosis. PDC-E2 is a well-characterized mitochondrial autoantigen central to the pathogenesis of Primary Biliary Cholangitis (PBC).
As a factory-direct manufacturer and supplier in China, we combine advanced assay technology with exceptional value and support. Each kit is available at competitive manufacturer-direct pricing, with bulk order discounts available. These products are part of our extensive portfolio of over 6,000 research assays, trusted through collaborations with more than 70 universities worldwide.
Product Overview: Two Critical Targets, One Reliable Source
Our kits are designed for researchers focusing on mitochondrial function, cell death mechanisms, and autoimmune diseases. Below is a clear comparison to help you select the right tool.
| Parameter | Mouse DLAT (PDC Component) ELISA Kit | Mouse PDC-E2 (Autoantigen) ELISA Kit |
|---|---|---|
| Primary Biological Role | Catalytic subunit of the Pyruvate Dehydrogenase Complex (PDC), linking glycolysis to the TCA cycle. Key player in cuproptosis, a copper-dependent cell death pathway. | The E2 component (dihydrolipoamide acetyltransferase) of PDC. The major mitochondrial autoantigen targeted by anti-mitochondrial antibodies (AMAs) in PBC. |
| Key Research Areas | Cancer metabolism (Warburg effect), cuproptosis mechanisms, metabolic syndrome, mitochondrial disorders. | Autoimmune disease research (Primary Biliary Cholangitis/PBC), autoantibody studies, liver immunology. |
| Detection Principle | Sandwich ELISA | Sandwich ELISA |
| Performance Tier | Available in Standard and High-Sensitivity (Biotin-Streptavidin) formats. | Available in Standard and High-Sensitivity (Biotin-Streptavidin) formats. |
| Assay Range | Please contact for specific datasheet. | 1.0 – 120 ng/mL |
| Sensitivity | Please contact for specific datasheet. | < 1.0 ng/mL |
| Optimal Sample Types | Tissue homogenates (e.g., liver, tumor), cell lysates. | Tissue homogenates (liver), cell lysates. Serum/plasma for autoantibody studies may require specific protocols. |
Core Features & Benefits
- Target-Specific Precision: Each kit utilizes highly specific capture and detection antibodies, ensuring accurate quantification of DLAT or PDC-E2 without significant cross-reactivity.
- Dual-Format Flexibility: Choose the performance level that matches your needs:
- Standard Format: Features an optimized one-step sandwich ELISA protocol with a specialized diluent to reduce matrix interference from complex lysates.
- High-Sensitivity Format: Employs a >95% pure biotinylated recombinant monoclonal antibody and Streptavidin-HRP conjugate (≥500 U/mg). This system provides superior signal amplification and directly mitigates background, ideal for low-abundance targets.
- Free Expert Testing Service: Purchasing any kit includes access to our professional lab. We begin analysis within 24 hours of receiving your samples and deliver a complete report (raw data, concentrations, standard curve) in ~5 business days.
- Exceptional Direct Value: Benefit from competitive manufacturer pricing without intermediary markups.
Our Commitment to Your Research
- Publish & Be Rewarded: Cite these ELISA Kits in your publication with a link to our site, and receive a complimentary standard ELISA kit (up to $120 value).
- 100% Satisfaction Guarantee: We offer an unconditional replacement or refund if a product does not meet its stated performance specifications.
- Dedicated 24-Hour Support: Our technical team provides a proposed solution within one business day.
- Integrity in Partnership: We handle your samples with the utmost care, recognizing their critical role in your scientific discoveries.
Assay Principle & General Specifications
Detection Principle: Both kits use a sandwich ELISA. The target protein (DLAT or PDC-E2) is captured between an immobilized antibody and an HRP-conjugated detection antibody. After washing, TMB substrate reaction produces a color change measured at 450 nm, proportional to the analyte concentration.
General Performance (Example based on provided PDC-E2 data):
- Accuracy: Correlation Coefficient R ≥ 0.9900.
- Precision: Intra- and Inter-assay CV < 15%.
- Specificity: High specificity with minimal cross-reactivity.
- Storage: 2-8°C, protected from light; 6-month shelf life.
Sample Handling: For tissue and cell samples, use appropriate lysis buffers. Clarify all lysates by centrifugation (e.g., 10,000-15,000×g is often recommended for mitochondrial proteins). Aliquot and store at -20°C or lower, avoiding repeated freeze-thaw cycles.
Ordering & Services
| Option | Description | Details |
|---|---|---|
| Kit Purchase (DLAT or PDC-E2) | Select your target and preferred tier (Standard or High-Sensitivity). | Contact for manufacturer price & bulk quotes. |
| Expert Testing Service | We perform the assay and provide a full analytical report. | ~5-day turnaround. Free with kit purchase. |
| Custom ELISA Development | Tailored solutions for novel research targets. | Project-based quotation. |
To order your Mouse DLAT or PDC-E2 ELISA Kit, request detailed specifications, or learn more about our services, contact Yanda Bio.
Research Applications & Frequently Asked Questions (FAQ)
Primary Applications (RUO):
- DLAT Kit: Research on cuproptosis, cancer cell metabolism (especially altered glucose metabolism), neurodegenerative diseases, and mitochondrial function.
- PDC-E2 Kit: Studying the immunopathology of Primary Biliary Cholangitis (PBC), characterizing mouse models of autoimmune liver disease, and autoantigen biology.
Q: Are DLAT and PDC-E2 the same protein?
A: They are closely related but distinct. PDC-E2 is one of the core enzymes within the PDC. DLAT is often used synonymously with PDC-E2, but strictly speaking, it refers specifically to the dihydrolipoamide acetyltransferase activity. Our kits are designed to quantify these specific targets as defined. Please confirm your exact target of interest.
Q: Can these kits be used for serum/plasma samples?
A: The primary application is for tissue or cell lysates where these mitochondrial proteins are expressed. Serum/plasma may contain autoantibodies against PDC-E2 (in PBC research) but very low levels of the native protein itself. Specialized protocols may apply.
Q: How do I claim the free kit for a publication?
A: Upon publication of your article citing the specific “Mouse DLAT/PDC-E2 ELISA Kit (Yanda Bio)” with a link to our product page, send us the citation details (DOI) to claim your complimentary kit.
Q: Why buy an ELISA test kit made in China from Yanda Bio?
A: As a direct manufacturer, we provide authentic pricing, complete control over quality, direct access to our R&D team for support, and a vast portfolio, ensuring you receive a high-value, reliable research tool.
Manufacturer’s Note
These products are for Research Use Only (RUO). They are not intended for diagnostic, therapeutic, or clinical procedures. Optimal results require careful sample preparation and adherence to the protocol.
