Mouse RANKL (TNFSF11) ELISA Kit Kit composition
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Mouse TNFSF11/RANKL ELISA Kit: Essential for Bone & Immunology Research
Yanda Bio’s Mouse TNFSF11/RANKL ELISA Kit is engineered for the specific and sensitive quantification of Tumor Necrosis Factor Ligand Superfamily Member 11 (TNFSF11), commonly known as RANKL (Receptor Activator of Nuclear Factor Kappa-B Ligand), in mouse serum, plasma, cell culture supernatants, and tissue lysates. As the master cytokine driving osteoclast differentiation and bone resorption, RANKL is a central research target in osteoporosis, rheumatoid arthritis, cancer metastasis to bone, and immune regulation. This robust kit provides crucial data for researchers in bone biology, immunology, and oncology.
As a certified factory-direct manufacturer with professional laboratories in China, we guarantee quality and value. This Mouse TNFSF11/RANKL ELISA Kit made in China is available at competitive manufacturer-direct pricing, with bulk orders priced under $100. It is part of our vast portfolio of over 6,000 detection targets, trusted through collaborative research with more than 70 universities worldwide.
Choose Your Performance Tier for Optimal Results
To address diverse research needs, we offer two optimized kit formats. Purchasing either tier includes our complimentary expert testing service.
| Feature | Tier 1: Standard Mouse RANKL ELISA Kit | Tier 2: High-Sensitivity Mouse RANKL ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with proprietary matrix-suppression diluent. | Biotinylated recombinant monoclonal antibody (>95% pure) combined with Streptavidin-HRP conjugate (≥500 U/mg). |
| Key Advantage | Excellent Value & Reliability. Features a simultaneous single-step incubation for efficiency. The specialized diluent actively reduces background interference from hemoglobin and lipids, ensuring consistent performance in complex samples. | Maximum Sensitivity & Specificity. The advanced biotin-streptavidin amplification system directly counteracts matrix effects, delivering superior signal-to-noise for detecting low pg/mL levels or analyzing challenging matrices. |
| Ideal For | Routine quantification in models where RANKL levels are robust (e.g., inflammatory bone disease). | High-precision studies requiring detection of subtle changes, low-abundance samples, or work with highly complex backgrounds. |
Included Expert Testing Service: Fast, Reliable Data
Streamline your workflow and ensure data integrity with our professional in-house service, included with every purchase.
- Rapid Turnaround: We initiate the assay within 24 hours of sample receipt.
- Comprehensive & Timely Reporting: You will receive a detailed data package in approximately 5 business days, containing raw OD values, calculated concentration tables, and a standard curve graph.
- Risk-Free Validation: Ideal for confirming assay performance with your specific mouse samples before establishing an in-house protocol.
Our Unwavering Commitment to Your Research
We build trust through actionable guarantees:
- Publication Reward: Cite this Mouse RANKL ELISA Kit with a link to our product page in your publication, and we will send you a complimentary standard ELISA kit (up to $120 value).
- 100% Performance Guarantee: We stand behind our products with an unconditional replacement or refund policy if specifications are not met.
- 24-Hour Support Pledge: Our dedicated team provides a clear resolution path within 24 hours of any inquiry.
- Guardians of Your Science: We treat every sample with precision and care, upholding the highest standards to support your scientific integrity.
Assay Principle & Technical Specifications
Detection Principle: The kit employs a validated one-step sandwich ELISA. Mouse RANKL is captured between an immobilized antibody and an HRP-conjugated detection antibody. Following washes, a TMB substrate reaction produces a color change measured at 450 nm, with intensity directly proportional to the RANKL concentration.
Performance Specifications:
- Sensitivity: < 10 pg/mL.
- Detection Range: 10.0 – 800 pg/mL.
- Accuracy (Linearity): Correlation Coefficient R ≥ 0.9900.
- Precision: Intra-assay & Inter-assay Coefficient of Variation (CV) < 15%.
- Specificity: No significant cross-reactivity with analogous molecules.
- Storage: 2-8°C, protected from light and moisture; 6-month shelf life.
Sample Handling: For serum/plasma, collect using standard procedures and centrifuge at 3000×g for 10-30 minutes. For tissues or cells, use appropriate lysis buffers. Aliquot and store all samples at -20°C or lower, avoiding repeated freeze-thaw cycles. A 1:5 sample dilution is typically required for serum/plasma.
Streamlined Workflow Overview
- Addition: Add 50 µL of standards or prepared samples to the pre-coated wells.
- Incubation: Add 100 µL of the HRP-conjugated Detection Antibody (except to blank well). Incubate at 37°C for 60 minutes.
- Wash: Wash the microplate 5 times thoroughly using the provided wash buffer.
- Development: Add 50 µL each of Substrate A and B. Incubate at 37°C for 15 minutes in the dark.
- Measurement: Add 50 µL Stop Solution. Read the absorbance at 450 nm within 15 minutes.
Ordering Information & Services
| Option | Description | Details |
|---|---|---|
| Tier 1: Standard Kit Purchase | Cost-effective, reliable solution for in-laboratory use. | Retail: $120. Bulk: < $100 (contact for quote). |
| Tier 2: High-Sensitivity Kit Purchase | Premium-grade kit for maximum sensitivity requirements. | Contact for competitive manufacturer price. |
| Expert Testing Service | Full-service assay execution and detailed report. | ~5-day turnaround. Free with kit purchase. |
| Custom ELISA Development | Tailored solutions from our portfolio of 6,000+ targets. | Project-based quotation available. |
To buy the Mouse TNFSF11/RANKL ELISA Kit online or request a quote, contact Yanda Bio today.
Research Applications & Frequently Asked Questions (FAQ)
Primary Applications (Research Use Only): Osteoporosis and bone metabolism research, rheumatoid arthritis and inflammatory joint disease models, cancer-induced bone disease (e.g., breast cancer metastasis), dental biology (periodontitis), and immune cell differentiation studies.
Q: What is the primary function of RANKL?
A: RANKL is the essential cytokine that binds to its receptor RANK on osteoclast precursors, triggering their differentiation, activation, and survival. It is the primary mediator of physiological and pathological bone resorption.
Q: How do I claim the free kit for a publication?
A: Once your peer-reviewed article citing the “Mouse TNFSF11/RANKL ELISA Kit (Yanda Bio)” with a direct link to this product page is published, send us the citation details (DOI). We will promptly fulfill your reward.
Q: What is the key difference between the two tiers?
A: The core difference is the detection system sensitivity and matrix tolerance. Tier 2’s biotin-streptavidin system provides significantly higher signal amplification, making it indispensable for detecting very low RANKL concentrations or for use with samples prone to high background.
Q: Why source from a manufacturer in China?
A: Sourcing directly from Yanda Bio in China ensures you receive the authentic manufacturer price, benefit from in-house R&D and quality control, and gain direct technical support, offering exceptional value without compromising on quality.
Manufacturer’s Note
This product is for Research Use Only (RUO). It is not intended for diagnostic, therapeutic, clinical, or animal clinical procedures. Researchers are responsible for validating the kit’s suitability for their specific experimental models.
