Mouse S1P ELISA Kit Materials supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by: 0,50, 100,200,400,800 nmol/ L
Reagent preparation
20×wash solution:Dilute with Distilled or deionized water 1:20.
Choose Your Optimal Assay Format
To accommodate diverse research needs, we offer two optimized kit formats. Purchasing either tier grants access to our complimentary expert testing service.
| Feature | Tier 1: Standard Mouse S1P ELISA Kit | Tier 2: High-Sensitivity Mouse S1P ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with proprietary background-suppression diluent. | Biotinylated recombinant monoclonal antibody (>95% pure) coupled with Streptavidin-HRP conjugate (≥500 U/mg). |
| Key Advantage | Cost-Effective & Highly Reliable (>99% Success Rate). Uses a simultaneous incubation step and a specialized diluent to reduce interference from hemoglobin and lipids, ensuring robust performance for routine analysis. | Maximum Sensitivity & Matrix Tolerance. The advanced biotin-streptavidin amplification system directly circumvents matrix effects, providing exceptional signal-to-noise for challenging samples. |
| Selection Guidance | Ideal for common targets. Choose this tier for its proven reliability, excellent value, and established performance in diverse experimental settings. | Recommended for precious samples or rare/low-abundance targets. Select this tier for the highest likelihood of first-attempt success with critical or limited samples. |
Complimentary Expert Testing Service: Accelerate Your Research
Leverage our professional laboratory expertise to save time and ensure data integrity. This service is included with every purchase.
- Rapid Processing: We initiate your assay within 24 hours of sample receipt.
- Comprehensive & Fast Reporting: You will receive a detailed data package in approximately 5 business days, including raw OD values, calculated concentration tables, and a standard curve graph.
- Confidence in Results: An ideal solution for validating assay performance with your specific mouse models.
Our Commitment to Your Scientific Success
We build trust through transparent and actionable guarantees:
- Publication Acknowledgement: Cite this Mouse S1P ELISA Kit with a link to our product page in your publication, and we will send you a complimentary standard ELISA kit (up to $120 value).
- 100% Performance Guarantee: We stand firmly behind our products. If a kit does not meet its published specifications, we offer an unconditional replacement or refund.
- 24-Hour Support Resolution: Our dedicated team provides a clear path toward resolution within 24 hours of any inquiry.
- Guardians of Your Research: We treat every sample with the utmost precision, understanding its vital role in your scientific progress.
Assay Principle & Technical Specifications
Detection Principle: The kit employs a validated one-step sandwich ELISA. Mouse S1P in the sample is captured between an immobilized antibody and an HRP-conjugated detection antibody. Following washes, a TMB substrate reaction produces a color change measured at 450 nm. The signal intensity is directly proportional to the S1P concentration.
Typical Performance Specifications:
- Sensitivity: < 10 pg/mL.
- Detection Range: 10 – 800 pg/mL.
- Accuracy (Linearity): Correlation Coefficient R ≥ 0.9900.
- Precision: Intra-assay & Inter-assay Coefficient of Variation (CV) < 15%.
- Specificity: High specificity with minimal cross-reactivity to analogous lipids.
- Storage: 2-8°C, protected from light and moisture; 6-month shelf life.
Sample Handling: For serum/plasma, collect using standard procedures and centrifuge at 3000×g for 10-30 minutes to clarify. For tissues, use appropriate extraction methods. Aliquot and store all samples at -20°C or lower, avoiding repeated freeze-thaw cycles. A 1:5 sample dilution is typically recommended.
Streamlined Assay Workflow
- Add Standards & Samples: Pipette 50 µL of standards or prepared samples into the antibody-coated wells.
- Add Detection Antibody & Incubate: Add 100 µL of HRP-conjugated Detection Antibody to all wells except the blank. Incubate at 37°C for 60 minutes.
- Wash Plate: Aspirate and wash the microplate 5 times thoroughly with the provided wash buffer.
- Develop Signal: Add 50 µL each of Substrate A and B. Incubate at 37°C for 15 minutes protected from light.
- Stop & Read: Add 50 µL Stop Solution. Measure the absorbance at 450 nm within 15 minutes.
Calculation: Generate a standard curve by plotting standard concentrations against their OD values. Interpolate sample concentrations from the curve, applying the appropriate dilution factor.
Ordering Information & Services
| Option | Description | Details |
|---|---|---|
| Tier 1: Standard Kit Purchase | Reliable, cost-effective solution for in-laboratory use. | Retail: $120. Bulk: < $100 (contact for quote). |
| Tier 2: High-Sensitivity Kit Purchase | Premium performance for maximum sensitivity needs. | Contact for competitive manufacturer price. |
| Expert Testing Service | Full-service assay execution and detailed analytical report. | ~5-day turnaround. Free with kit purchase. |
| Custom ELISA Development | Tailored assay solutions from our portfolio of 6,000+ targets. | Project-based quotation available. |
To buy the Mouse S1P ELISA Kit or request a quote, contact Yanda Bio, your trusted direct supplier.
Research Applications & Frequently Asked Questions (FAQ)
Primary Applications (Research Use Only): Immunology & inflammation studies (lymphocyte egress), cardiovascular research (endothelial barrier function), cancer biology (tumor microenvironment), neuroscience (neuroinflammation, multiple sclerosis models), and metabolic disorder research.
Q: Why is S1P an important research target?
A: S1P is a master regulator of lymphocyte circulation, vascular stability, and cell survival. Its gradients between tissues and blood are critical for immune function, and its dysregulation is implicated in autoimmune diseases, cancer, and vascular disorders.
Q: How do I decide between Tier 1 and Tier 2?
A: Follow our Selection Guidance. For well-established research where sample is abundant, Tier 1 offers outstanding value and reliability. For projects involving rare samples, low-concentration measurements, or where experimental success is critical, Tier 2 provides the necessary sensitivity and robustness.
Q: How do I claim the free kit for a publication?
A: Upon publication of your article citing the “Mouse Sphingosine-1-phosphate (S1P) ELISA Kit (Yanda Bio)” with a direct link to this product page, send us the citation details (DOI). We will promptly fulfill your complimentary kit reward.
Q: Why choose Yanda Bio as your supplier from China?
A: As a direct manufacturer, we ensure authentic manufacturer pricing, complete in-house quality control, direct access to technical support, and a vast portfolio, providing you with a high-value, reliable research tool without intermediary costs.
Manufacturer’s Note
This product is for Research Use Only (RUO). It is not intended for diagnostic, therapeutic, clinical, or animal clinical procedures. Optimal results depend on proper sample preparation and adherence to the protocol.
