Mouse 5α-Reductase (5AR) ELISA Kit contain
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0,20,40,80, 160,320 ng/ml
Accurately Quantify Mouse 5α-Reductase (5AR) for Hormone & Disease Research
Yanda Bio’s Mouse 5α-Reductase (5AR) ELISA Kit provides a specific and sensitive assay for the quantitative measurement of 5α-Reductase protein in mouse tissue lysates (e.g., prostate, liver), cell extracts, and serum. This key enzyme catalyzes the irreversible conversion of testosterone to the more potent androgen, dihydrotestosterone (DHT), making it a critical focus in research on prostate biology, androgenetic alopecia, and endocrine-related disorders.
As a factory-direct manufacturer, we offer premium ELISA kit production at transparent, competitive prices. This kit serves as a reliable and cost-effective ELISA kit alternative to suppliers like Abcam, delivering comparable performance without the premium cost. Optimize your research budget without compromising on data quality.
High-quality microplates are the key to the success of the experiment.
The microplate included in this one-step sandwich mouse 5α-reductase (5AR) ELISA kit is engineered for high sensitivity, reproducibility, and convenience. Key features of the pre-coated plate include:
1. Material & Physical Design
- Material: Manufactured from high-quality, imported polystyrene with excellent protein adsorption capacity, ensuring strong and consistent binding.
- Format: A standard 96-well plate with a detachable strip design (8 wells × 12 strips). This allows you to use only the required number of strips, minimizing waste and supporting cost-effective assay runs.
- Well shape: Flat-bottom wells. The low refractive index of flat-bottom plates ensures optimal compatibility with microplate readers, delivering accurate optical density (OD) readings.
2. High-Binding Surface & Pre-Coating
- Surface treatment: The plate features a high-binding surface that can bind approximately 300–400 ng IgG/cm², specifically optimized for proteins larger than 10 kDa.
- Pre-coated capture antibody: Each well is pre-coated with a specific capture antibody targeting mouse 5α-reductase (5AR). Thanks to the high-binding surface, the capture antibody is firmly immobilized, enabling efficient antigen capture in a single incubation step and simplifying the one-step sandwich workflow.
3. Optical Compatibility for Colorimetric Detection
- Plate color: Transparent, designed for standard colorimetric ELISA detection.
- Detection wavelength: Used with an HRP-labeled detection antibody and TMB substrate (which produces a yellow color upon reaction), the transparent plate is optimized for absorbance measurement at 450 nm with a standard microplate reader.
4. Precision & Assay Reproducibility
- Uniformity: Advanced manufacturing ensures uniform well thickness and diameter across the plate. This minimizes well-to-well variation, significantly improving intra-assay precision and data reproducibility for your quantitative mouse 5AR measurements.
Select the Perfect Assay for Your Needs: Value vs. Ultra-Sensitivity
To support diverse research goals, we offer two distinct Mouse 5AR ELISA Kit formats. Purchasing either tier grants access to our complimentary expert sample testing service.
| Feature | Tier 1: Standard 5AR ELISA Kit | Tier 2: High-Sensitivity 5AR ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with a proprietary, interference-reducing diluent. | Biotinylated recombinant monoclonal antibody (>95% pure) + Streptavidin-HRP conjugate (≥500 U/mg). |
| Key Advantage | Exceptional Value & Proven Reliability (>99% success rate). Uses a simultaneous incubation step. The specialized diluent minimizes background from hemoglobin and lipids, ensuring robust performance for routine analysis. | Maximum Sensitivity & Matrix Resistance. The advanced biotin-streptavidin system directly overcomes matrix effects, providing superior signal-to-noise for the most demanding applications. |
| Our Selection Advice | Ideal for common targets and standard research. Choose this tier for outstanding value, with a retail price of $120 and bulk pricing under $100. | Recommended for precious/rare samples or low-abundance targets. Invest in this tier to maximize the chance of first-attempt success with critical experiments. |
| Best For | High-throughput studies, routine biomarker analysis, and budget-conscious projects. | Studies with limited sample volume, detecting subtle expression changes, or analyzing complex tissue lysates. |
Complimentary Expert Testing Service
Validate your assay or save time with our professional service, included with every kit purchase.
- Fast Turnaround: Begin analysis within 24 hours of sample receipt; receive a full report in ~5 business days.
- Complete Data Package: Get raw OD values, concentration tables, and a standard curve graph.
Why Choose Yanda Bio’s 5AR ELISA Kit?
- Direct Manufacturer Price: Source Mouse 5AR ELISA Kit with manufacturer price directly, eliminating distributor markups.
- Custom & Production Flexibility: We offer ELISA kit custom service and reliable ELISA kit production for specialized needs.
- Publication Reward: Cite this kit with a link to our site in your publication, and receive a free standard ELISA kit.
- 100% Satisfaction Guarantee: We promise performance with an unconditional replacement or refund policy.
- 24-Hour Support: Get a proposed solution within one business day.
Assay Principle & Specifications
Detection Principle: This sandwich ELISA captures mouse 5AR between an immobilized antibody and an HRP-conjugated detection antibody. TMB substrate reaction, measured at 450 nm, yields a signal proportional to 5AR concentration.
Performance Specifications:
- Sensitivity: < 1.0 ng/mL.
- Detection Range: 1.0 – 320 ng/mL.
- Accuracy: R ≥ 0.9900.
- Precision: Intra- & Inter-assay CV < 15%.
- Specificity: High specificity with minimal cross-reactivity.
- Storage: 2-8°C; 6-month shelf life.
Sample Prep: Use appropriate lysis buffers for tissues/cells. For serum/plasma, centrifuge at 3000×g for 10-30 mins. Aliquot and store at -20°C, avoiding freeze-thaw cycles. A 1:5 dilution is typically required.
Ordering & Custom Services
| Option | Description | Details |
|---|---|---|
| Tier 1: Standard Kit | Cost-effective solution for common research needs. | Retail: $120. Bulk: <$100 (quote required). |
| Tier 2: High-Sensitivity Kit | Premium performance for critical applications. | Contact for manufacturer price. |
| Expert Testing Service | We run the assay for you. | Free with kit purchase. |
| Custom ELISA Development | Tailored Mouse 5AR ELISA Kit customed to your specs. | Project-based quote. |
Ready to buy a reliable, cost-effective Mouse 5AR ELISA Kit? Contact Yanda Bio today for pricing or to request a custom quote.
Related Androgen Mouse ELISA Kit:
- Mouse 3-Indolelactic Acid (ILA) ELISA Kit
- Mouse FDX1 (Adrenodoxin) ELISA Kit——Ferredoxin 1 Quantitation from Direct Manufacturer
Research Applications & FAQ
Applications (RUO): Prostate disease research (BPH, cancer), studies on androgenetic alopecia, endocrine disruption, steroid metabolism, and liver function.
Q: Is this kit for protein concentration or enzymatic activity?
A: This ELISA kit measures the protein concentration of 5α-Reductase. For measuring enzymatic activity, a specific activity assay is required.
Q: How does this kit compare to Abcam’s?
A: Our Mouse 5AR ELISA Kit offers comparable sensitivity and specificity as a cost-effective elisa kits alternatives to abcam, with the added benefits of direct manufacturer support, bulk pricing under $100, and a free testing service.
Q: Can this kit be used for rat samples?
A: It is validated for mouse samples. Cross-reactivity with rat 5AR is not guaranteed. Please inquire about our Elisa Kit custom service for species-specific validation or development.
Q: How do I claim the free publication kit?
A: Send us the citation (DOI) of your published article that cites “Mouse 5AR ELISA Kit (Yanda Bio)” and links to our product page.
Manufacturer’s Note
For Research Use Only (RUO). Not for diagnostic use.
