The Rat S1P ELISA Kit supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by: 0,50, 100,200,400,800 nmol/ L
Understanding Sphingosine-1-phosphate (S1P)
Sphingosine-1-phosphate (S1P) is a potent bioactive lipid mediator with critical roles in immune cell trafficking, vascular integrity, angiogenesis, and neurological function. Precise quantification of Rat S1P levels is essential for research in immunology, inflammation, cardiovascular biology, cancer, and neurodegenerative diseases.
Yanda Bio’s Rat Sphingosine-1-phosphate (S1P) ELISA Kit is meticulously validated to provide researchers with reliable, reproducible data for this pivotal signaling molecule. As a leader in ELISA Kit production, with a portfolio exceeding 6,000 detectable targets and enduring collaborations with over 60 universities, we deliver premium, cost-effective rat ELISA Kit solutions to drive scientific discovery forward.
Exceptional Quality, Direct-to-Researcher Value
High-cost ELISA kits often justify their price through the use of high-affinity antibodies and optimized reagents. At Yanda Bio, we believe premium performance should be accessible.
Our Core Advantage: We source and utilize high-specificity antibodies and high-activity enzyme conjugates equivalent in quality to those found in leading international brands. By streamlining our efficient ELISA Kit production and supply chain, we integrate these superior components into a genuinely low-cost ELISA Kit model. This ensures you receive exceptional sensitivity and specificity for your S1P research without the premium price tag.
Dual-Format Design: Tailored for Your Research Need & Budget
To accommodate diverse experimental goals—from high-throughput screening to analysis of precious samples—we offer two optimized assay formats.
Format 1: Standard Performance Kit
Engineered for robust, cost-effective analysis of S1P in standard research settings. This kit employs a simplified one-step incubation protocol and a proprietary matrix optimization buffer. The specialized diluent effectively reduces interference from hemoglobin, lipids, and other common sample components, ensuring low background noise and high data consistency. With a validated success rate exceeding 99%, it is the ideal cost-effective choice for routine quantification.
- Typical Investment: Approximately $120 (retail). Less than $100 per kit for volume or wholesale purchases.
Format 2: Ultra-Sensitive Kit
Designed for the most demanding applications where the sample is limited, or S1P concentrations are expected to be very low. This advanced format leverages a biotinylated recombinant monoclonal antibody (purity >95%) coupled with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This powerful signal amplification system maximizes detection sensitivity, directly mitigates matrix effects, and is built to guarantee success in critical, one-attempt experiments.
Selecting the Right Format:
- Choose Format 1 for reliable, high-value measurement in studies with abundant sample material.
- Choose Format 2 when working with trace analyte levels, irreplaceable biopsies, or complex sample matrices requiring the highest level of assay robustness.
Assay Principle & Protocol Summary
This kit is based on a well-established sandwich enzyme-linked immunosorbent assay (ELISA) principle. A capture antibody specific for Rat S1P is pre-coated onto the microplate. S1P present in standards or samples binds to the immobilized antibody. After washing, an HRP-conjugated detection antibody is added to form an antibody-antigen-antibody complex. Subsequent addition of TMB substrate produces a blue color, which turns yellow upon stopping. The intensity of the yellow color (OD at 450nm) is directly proportional to the concentration of S1P in the sample.
Key Procedure Notes: All reagents should be equilibrated to room temperature before use. The provided wash buffer concentrate must be diluted accurately. Consistent timing during incubation and thorough washing are critical for optimal performance and reproducibility.
Commitment to Researcher Success
- Premium Components, Affordable Price: Access top-tier immunoassay performance through our efficient, value-driven, cost-effective rat ELISA Kit model.
- Free Expert Testing Service: Purchase any Yanda Bio ELISA kit and receive FREE data generation service—simply send us your prepared samples.
- Publication Incentive Program: Acknowledge the use of our kit with a citation and direct link to this product page in your publication to receive a complimentary standard ELISA kit.
- 100% Satisfaction Guarantee: We stand firmly behind our products. If performance does not meet the specifications detailed herein, we offer an unconditional replacement or full refund.
- Dedicated 24/7 Support: Our technical support team is committed to providing a detailed response and proposed solution within 24 hours of your inquiry.
- Fully Customizable Solutions: Need an assay not in our extensive catalog? Inquire about our comprehensive custom ELISA kit development services.
Technical Specifications & Performance Data
- Accuracy: Linear regression correlation coefficient (R) of the standard curve ≥ 0.9900.
- Precision: Intra-assay Coefficient of Variation (CV) < 10%; Inter-assay CV < 15%.
- Specificity: Demonstrates no significant cross-reactivity with other relevant sphingolipid analogs.
- Sample Compatibility: Fully validated for use with rat serum, plasma (EDTA, citrate, heparin), cell culture supernatant, and tissue homogenate.
- Kit Stability: Stable for 6 months from the date of manufacture when stored continuously at 2-8°C.
