Product Overview: Understanding Dihydrotestosterone in Research
Dihydrotestosterone (DHT) is the most potent naturally occurring androgen, synthesized from testosterone by the enzyme 5α-reductase. It plays a critical role in male sexual development, prostate growth, hair follicle biology, and endocrine signaling. Dysregulation of DHT levels is implicated in benign prostatic hyperplasia (BPH), prostate cancer, androgenetic alopecia, polycystic ovary syndrome (PCOS), and various endocrine disorders. Accurate quantification of DHT is essential for research in endocrinology, reproductive biology, urology, dermatology, and pharmacological studies targeting androgen signaling pathways.
Yanda Bio’s Customized Dihydrotestosterone (DHT) ELISA Kit is a competitive immunoassay optimized for sensitive and specific detection of this critical androgen. As a professional ELISA kit manufacturer with a portfolio exceeding 6,000 detectable targets and established collaborations with over 60 universities across China, we combine deep endocrinology expertise with rapid customization capabilities.
Explore Our Related Androgen Assays:
Why Choose Yanda Bio for Your Custom Androgen Immunoassays?
EEAT-Compliant Quality Standards
Our commitment to Experience, Expertise, Authoritativeness, and Trustworthiness ensures that every custom kit meets the highest research standards for steroid hormone quantification.
| Principle | Our Commitment |
|---|---|
| Experience | Over 6,000 detectable factors developed; 60+ university collaborations; years of specialized expertise in steroid hormone immunoassay manufacturing and validation. |
| Expertise | Specialized R&D team with proprietary steroid conjugation and antibody development protocols; proven expertise in optimizing assays for complex biological matrices. |
| Authoritativeness | Validated by academic research partners; publications citing our kits; ISO-aligned manufacturing standards; rigorous quality control at every development stage. |
| Trustworthiness | Rapid custom service with 3-7 day turnaround; transparent specifications; unconditional quality guarantee; reliable technical support from development through application. |
Rapid Custom Development for Hormone Research
Need an assay for a specific species, sample type, or detection range? Yanda Bio offers custom ELISA kit development services with a rapid 3-7 day turnaround—ideal for specialized endocrinology applications. Our team will work with you to:
- Optimize antibody specificity for DHT versus other androgens
- Validate performance with your specific sample matrix (serum, tissue, cell culture)
- Establish appropriate detection ranges for your experimental model
- Provide complete documentation and technical support
Superior Quality Metrics
- Intra-assay CV < 10% — Exceptional within-run precision
- Inter-assay CV < 15% — Reliable run-to-run consistency
- High Specificity — Minimal cross-reactivity with testosterone, androstenedione, and other steroid hormones
Two Optimized Kit Formats for Your Research Needs
To accommodate diverse experimental requirements and budget considerations, we offer this customized DHT ELISA kit in two specialized formats.
Format 1: Standard High-Efficiency Kit
Designed for routine, reliable quantification of DHT in standard research settings. This format employs a streamlined one-step incubation where antigen binds simultaneously with the solid-phase antibody and enzyme-labeled antibody. A proprietary sample diluent is included to effectively minimize interference from common matrix components such as lipids and binding proteins, ensuring stable baseline signals and optimal binding. With extensive validation demonstrating a success rate above 99%, this format represents exceptional value for most applications.
- Typical Pricing: Approximately $120 (retail). Bulk pricing available under $100 per kit.
- Special Offer: Free shipping on orders of three or more ELISA kits.
Format 2: Advanced High-Sensitivity Kit
Engineered for demanding applications where sample material is precious, limited, or where detection of very low DHT concentrations is required (e.g., female serum, pediatric samples, or small tissue biopsies). This advanced format utilizes a biotinylated recombinant monoclonal antibody (purity >95%) paired with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This signal amplification system delivers superior sensitivity, directly circumvents matrix effects, and is designed to ensure success in critical, one-attempt experiments.
Selection Guide:
- Choose Format 1 for cost-effective, reliable analysis in routine endocrine studies with sufficient sample volume
- Choose Format 2 when working with irreplaceable samples, detecting subtle changes in low-level androgens, or requiring maximum assay robustness
Assay Principle: Competitive ELISA Technology for Steroid Hormones
This kit employs a competitive inhibition enzyme immunoassay format, the gold standard for small molecule steroid hormones where sandwich formats are not feasible. The microplate is pre-coated with an antibody specific for Dihydrotestosterone. During incubation, DHT in standards or samples competes with HRP-labeled DHT (the tracer) for binding to the immobilized antibody. After washing to remove unbound tracer, TMB substrate is added. The HRP enzyme catalyzes a colorimetric reaction, producing a blue product that turns yellow upon acidification. The intensity of the color is inversely proportional to the concentration of DHT in the sample—higher DHT concentrations result in lower color intensity.
Sample Dilution Note: Samples are pre-diluted 2-fold during the procedure; multiply results by 2 to obtain actual concentrations.
Sample Collection & Preparation
Proper sample handling is critical for accurate steroid hormone quantification. Please follow these guidelines:
Liquid Samples
| Sample Type | Collection & Processing |
|---|---|
| Serum | Use sterile pyrogen/endotoxin-free tubes. Allow blood to clot for 120 minutes at room temperature or overnight at 2-8°C. Centrifuge at 3000 rpm for 20 minutes at 2-8°C. Carefully collect supernatant. Store at -20°C or -80°C. Avoid repeated freeze-thaw cycles. |
| Plasma | Collect using EDTA or heparin sodium as anticoagulant. Mix thoroughly. Centrifuge at 3000 rpm for 20 minutes at 2-8°C within 30 minutes of collection. Carefully collect supernatant. Store at -20°C or -80°C. |
| Urine, CSF, Saliva | Collect using sterile tubes. Centrifuge at 3000 rpm for 20 minutes at 2-8°C. Carefully collect supernatant. Store at -20°C or -80°C. |
| Cell Culture Supernatant | Collect using sterile tubes. Centrifuge at 3000 rpm for 20 minutes at 2-8°C. Carefully collect supernatant. Store at -20°C or -80°C. |
Solid Samples
| Sample Type | Processing Protocol |
|---|---|
| Animal Tissue | Rinse with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood. Weigh and mince tissue. Add PBS at 1:9 weight-to-volume ratio (1g tissue to 9mL PBS). Homogenize on ice, then centrifuge at 5000×g for 10 minutes at 2-8°C. Collect supernatant. |
| Adherent Cells | Wash with pre-chilled PBS, detach with trypsin, and collect by centrifugation (1000×g, 5 min). Wash cells 3 times with cold PBS. Resuspend at 1×10⁷ cells/mL in cold PBS. Lyse by sonication. Centrifuge at 3000 rpm for 20 minutes at 2-8°C. Collect supernatant. |
| Suspension Cells | Collect by centrifugation, wash 3 times with cold PBS, then follow same procedure as adherent cells. |
Important Sample Notes
- Extract samples as soon as possible after collection. Perform assays promptly after extraction.
- For short-term storage (up to 6 days), samples can be stored at 2-8°C.
- For long-term storage, freeze at -20°C (≤1 month) or -80°C (≤2 months). Avoid repeated freeze-thaw cycles.
- If precipitate forms during storage, centrifuge again before testing.
- Do not use samples containing sodium azide (NaN₃), as it inhibits horseradish peroxidase (HRP) activity.
Materials Required But Not Provided
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes and tips (0.5-10 μL, 2-20 μL, 20-200 μL, 200-1000 μL)
- 37°C incubator or water bath
Important Notes for Assay Procedure
- Store the kit at 2-8°C. Allow all reagents to equilibrate to room temperature for 20 minutes before use. Concentrated wash buffer may crystallize; this is normal. Dissolve crystals by warming in a water bath.
- Return unused strips to the resealable bag with desiccant and store properly at 2-8°C. Use as soon as possible.
- The S0 standard (zero concentration) serves as a negative control/blank. Note that samples are pre-diluted 2-fold during the procedure; multiply results by 2 to obtain actual concentrations.
- Strictly adhere to the incubation times, volumes, and sequence specified in the protocol.
- Mix all liquid components thoroughly before use.
Reagent Preparation
20× Wash Buffer Dilution: Dilute 1 part 20× wash buffer with 19 parts distilled water to prepare 1× working wash buffer.
Washing Method
Manual Washing: Aspirate liquid from wells, fill each well with wash buffer, allow to stand for 1 minute, then aspirate. Invert plate and tap dry on absorbent paper. Repeat 5 times.
Automated Plate Washer: Inject 350 μL of wash buffer per well, allow to soak for 1 minute, and wash 5 times.
Assay Procedure
- Remove the required number of strips from the aluminum foil bag after 20 minutes of room temperature equilibration. Reseal unused strips and return to 4°C.
- Set up standard wells and sample wells. Add 50 μL of each standard concentration to the standard wells.
- For sample wells: Add 25 μL of test sample followed by 25 μL of sample diluent.
- Add 50 μL of HRP-labeled competitive antigen (tracer) to both standard and sample wells. Seal with a plate cover and incubate at 37°C for 60 minutes.
- Aspirate liquid, tap dry on absorbent paper, fill each well with wash buffer, let stand for 1 minute, aspirate, and tap dry. Repeat washing 5 times (automated washer acceptable).
- Add 50 μL of Substrate A and 50 μL of Substrate B to each well. Incubate at 37°C in the dark for 15 minutes.
- Add 50 μL of stop solution to each well. Measure the OD at 450 nm within 15 minutes.
Data Analysis
This competitive ELISA requires calculation of percent binding for accurate quantification.
Calculation Steps:
- Read OD Values: Record absorbance at 450 nm within 15 minutes of adding stop solution.
- Calculate Percent Binding:
- Let B0 = OD of S0 standard (zero concentration)
- Let B = OD of each standard or sample
- Let NSB = Non-specific binding (if applicable)
- Percent binding = (B / B0) × 100%
- Construct Standard Curve:
- Plot standard concentrations (x-axis, log scale) against percent binding (y-axis, linear scale) using logit-log or 4-parameter logistic (4PL) curve fitting.
- Calculate logit values: logit = ln[B/B0 / (1 – B/B0)]
- Determine Sample Concentrations:
- Calculate percent binding for each sample
- Interpolate concentration from the standard curve
- Multiply by the dilution factor (2×) to obtain actual sample concentration
Automated Analysis: Use ELISA data analysis software with logit-log or 4PL models for automatic curve fitting and concentration calculation.
Kit Performance Specifications
| Parameter | Performance |
|---|---|
| Accuracy | Standard curve linear regression coefficient (R) ≥ 0.9900 |
| Sensitivity | Minimum detectable concentration < 5 ng/mL (customizable) |
| Detection Range | 50 – 2000 ng/mL (customizable based on species and sample type) |
| Specificity | High specificity with minimal cross-reactivity to testosterone (< 1%), androstenedione (< 0.5%), and other steroid hormones |
| Intra-assay CV | < 10% |
| Inter-assay CV | < 15% |
| Storage | 2-8°C, protected from light and moisture |
| Shelf Life | 6 months |
Customization Options
As a Customized Dihydrotestosterone ELISA Kit, this product can be tailored to your specific research requirements:
| Custom Parameter | Available Options |
|---|---|
| Target Species | Human, mouse, rat, rabbit, horse, or custom species |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, urine, saliva |
| Detection Range | Adjustable based on expected physiological levels |
| Sensitivity | Optimized for your specific application (low-range options available) |
| Kit Format | Standard or high-sensitivity (biotin-streptavidin amplification) |
| Custom Controls | Species-specific matrix-matched controls available |
Custom Development Timeline: 3-7 days from specification confirmation to prototype delivery.
Special Offer
Purchase any three ELISA kits from Yanda Bio and enjoy free shipping. Combine our Customized DHT kit with our Testosterone and Androstenedione kits to build a comprehensive androgen research panel at exceptional value.
Comparison with Premium International Brands
Yanda Bio’s custom ELISA kits deliver performance comparable to leading international brands at a fraction of the cost—often 40-60% less than major commercial alternatives. Our efficient manufacturing process and direct-to-researcher business model eliminate unnecessary markups, allowing us to offer professional-grade steroid hormone immunoassays at highly competitive prices. With our 3-7 day custom development capability, we also deliver tailored solutions far faster than traditional custom service providers.
Disclaimer
- This product is for research use only. It is not intended for clinical diagnosis or human use. The user assumes all responsibility for any consequences arising from misuse.
- Strict adherence to the protocol is required. Yanda Bio is not responsible for results obtained from deviations from the specified procedure.
Ordering & Contact Information
To order this Customized Dihydrotestosterone (DHT) ELISA Kit, or to inquire about custom development for other steroid hormones, volume discounts, and format selection guidance, please contact our sales team.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
