The Human 5-Alpha Reductase (5AR) ELISA Kit enables quantitative measurement of 5α‑reductase protein levels, providing essential insights into androgen metabolism and related pathologies. In humans, three isoenzymes exist (SRD5A1, SRD5A2, and SRD5A3), with SRD5A1 and SRD5A2 primarily responsible for the NADPH‑dependent reduction of testosterone to the more potent androgen, dihydrotestosterone (DHT). Because DHT is a critical activator of the androgen receptor, this human 5‑alpha reductase ELISA kit is widely applied in prostate disease research to investigate the pathogenesis of benign prostatic hyperplasia (BPH) and prostate cancer, and to evaluate the pharmacodynamics of 5AR inhibitors such as finasteride and dutasteride in androgen‑dependent conditions. The kit is equally valuable in androgenetic alopecia and dermatological research for studying the mechanisms behind hair follicle miniaturization and excessive sebum production in acne, as well as for assessing the efficacy of hair‑loss treatments and cosmetic interventions. Furthermore, it supports endocrinology and developmental biology studies by enabling the analysis of steroid hormone biosynthesis and metabolic pathways, including the investigation of inherited disorders of sexual development caused by loss‑of‑function mutations in SRD5A2. With its straightforward protocol and high sensitivity, this human 5AR ELISA kit is a robust research tool for laboratories focused on androgen biology, endocrinology, and dermatology.
Choose Your Performance Tier for Optimal Results
We offer two optimized formats to suit your specific research requirements. Purchase any tier and gain access to our complimentary expert testing service.
| Feature | Tier 1: Standard 5AR ELISA Kit | Tier 2: High-Sensitivity 5AR ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with proprietary dilution buffer. | Biotinylated recombinant mAb (>95% pure) + Streptavidin-HRP (≥500 U/mg). |
| Key Advantage | Cost-Effective & Robust. Utilizes a simultaneous antigen-antibody incubation and a specialized diluent engineered to reduce interference from hemoglobin and lipids, ensuring stable, low-background performance. | Ultimate Precision & Sensitivity. The biotin-streptavidin signal amplification system directly overcomes matrix effects, delivering superior signal-to-noise for the most challenging samples or low-abundance targets. |
| Ideal For | Routine quantification in standard research models where sample material is not limiting. | Applications demanding the highest sensitivity, such as low-volume or low-expression samples. |
Complimentary Expert Testing Service: Your Fast Track to Data
Maximize efficiency and ensure data quality with our professional lab service, included with your purchase.
- Rapid Processing: Your experiment begins within 24 hours of sample receipt. You receive a comprehensive data package in as fast as 5 business days.
- Complete Analysis Report: The package includes raw OD data, calculated concentration tables, and a standard curve graph for immediate analysis.
- Risk-Free Validation: Ideal for confirming assay suitability before committing to large-scale in-house studies.
Our Foundational Commitment to Research Excellence
We build lasting partnerships through concrete actions that support your scientific integrity:
- Publish & Be Rewarded: Cite this Human 5AR ELISA Kit in your publication with a link to our site, and receive a free standard ELISA kit (up to $120 value) as our thanks.
- 100% Performance Guarantee: If a product does not meet its stated specifications, we offer an unconditional replacement or refund.
- Dedicated Support Pledge: Our team provides a proposed solution within 24 hours of any inquiry.
- Guardians of Your Discovery: We treat every sample with the respect it deserves, applying rigorous standards to safeguard the integrity of your research journey.
Technical Specifications & Assay Principle
Principle: This kit employs a validated one-step sandwich ELISA. 5α-Reductase present in the sample is captured between an immobilized antibody and an enzyme-conjugated detection antibody. Subsequent reaction with TMB substrate produces a color change measurable at 450 nm, proportional to the enzyme concentration.
Performance Specifications:
- Sensitivity: < 1.0 ng/mL.
- Detection Range: 1.0 – 200 ng/mL.
- Accuracy (Linearity): Correlation Coefficient R ≥ 0.9900.
- Precision: Intra- & Inter-assay CV < 15%.
- Specificity: Minimal cross-reactivity.
- Storage: 2-8°C; 6-month shelf life.
Sample Preparation: For cell and tissue samples, use appropriate lysis buffers. Clarify all samples by centrifugation (3000×g for 10 mins) and store at -20°C in aliquots. Serum may require specific dilution per protocol.
Streamlined Assay Workflow
- Addition: Add 50 µL of standards or prepared samples to the pre-coated wells.
- Detection & Incubation: Add 100 µL of the HRP-conjugated Detection Antibody. Incubate at 37°C for 60 minutes.
- Wash: Aspirate and wash the plate 5 times thoroughly.
- Development: Add 50 µL each of Substrate A and B. Incubate at 37°C for 15 minutes in the dark.
- Measurement: Add 50 µL Stop Solution. Measure absorbance at 450 nm within 15 minutes.
Related Products & Internal Link
Other recommended androgens and steroid hormone kits available from Yanda Bio:
- Human Dihydrotestosterone (DHT) ELISA Kit – High‑Specificity Sandwich Assay for Androgen Research
- Human Estrone-3-sulfate (E3S) ELISA Kit – Quantitative Sulfated Estrogen Immunoassay for Pharmacological & Oncology Research
- Customized Dihydrotestosterone (DHT) ELISA Kit – High-Specificity Competitive Assay for Androgen Research
Pricing & Services from Your Direct Supplier
| Option | Description | Lead Time / Price |
|---|---|---|
| Tier 1: Standard Kit Purchase | Reliable, value-driven solution for in-lab use. | Retail: $120. Bulk: < $100 (quote required). |
| Tier 2: High-Sensitivity Kit Purchase | Premium performance for maximum sensitivity needs. | Competitive manufacturer price (contact us). |
| Expert Testing Service | We run the assay and provide a complete data report. | ~5 business days. Free with kit purchase. |
| Custom ELISA Development | Tailored solutions from our portfolio of 6,000+ targets. | Project-based quotation. |
To order your Human 5AR ELISA Kit or explore our vast assay portfolio, contact Yanda Bio, your trusted direct manufacturer in China.
Research Applications & Frequently Asked Questions (FAQ)
Primary Applications (RUO): Benign prostatic hyperplasia (BPH) research, androgenetic alopecia studies, prostate cancer biology, steroid metabolism, and endocrine-disrupting compound analysis.
Q: What does this kit measure?
A: This ELISA kit quantifies the protein concentration of 5-alpha reductase. For measuring enzymatic activity, specific activity assay kits are recommended. Please confirm your experimental goal.
Q: How do I access your portfolio of 6,000+ assays?
A: Contact our technical support team with your target of interest. We can quickly check availability or discuss custom development options for novel targets.
Q: How do I claim the free kit for publication?
A: Upon publication of your article citing the “Human 5-Alpha Reductase (5AR) ELISA Kit (Yanda Bio)” with a link to our product page, send us the citation details to claim your complimentary kit.
Q: How can I buy this ELISA kit from China?
A: As the factory-direct source in China, you can buy this Human 5AR ELISA Kit online by contacting Yanda Bio directly. We manage seamless global logistics and documentation.
Manufacturer’s Note
This product is for Research Use Only (RUO). Not for diagnostic or clinical use. Researchers are responsible for validating the kit’s suitability for their specific sample types and experimental models.
