The Mouse 5-Hydroxytryptamine (5-HT) ELISA Kit provides sensitive and quantitative measurement of serotonin (5-HT) levels across a broad range of mouse biological samples, including serum, plasma, tissue homogenates, and cell culture supernatants or cell/tissue lysates. Please note the impact of the cell lysis solution on the experimental results.As a critical monoamine neurotransmitter, 5-hydroxytryptamine regulates essential physiological functions such as mood, sleep, and appetite, with dysregulated serotonin levels closely linked to depression, anxiety, and other neuropsychiatric disorders. This mouse serotonin ELISA kit is therefore widely applied in neuroscience research to profile neurotransmitter dynamics and investigate the regulatory mechanisms underlying emotion and sleep physiology. It is equally valuable in pharmacological studies for evaluating the effects of drug candidates, including SSRIs and other serotonergic agents, on 5-HT concentrations, as well as in disease model research for validating behavioral phenotypes and exploring metabolic pathways in relevant rodent models. With its standardized sandwich ELISA workflow, this kit delivers reproducible, high-throughput results for neurobiology, pharmacology, and disease mechanism investigations.
The Mouse 5-HT ELISA Kit consists of the following components
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0,30,60, 120,240,480 ng/mL
Value Without Compromise: Choose Your Precision Tier
Our Mouse 5-Hydroxytryptamine (5-HT) assay is available in two optimized formats to suit your sensitivity requirements and budget. Purchasing either tier includes our complimentary expert sample testing service.
| Feature | Tier 1: Standard Mouse 5-HT ELISA Kit | Tier 2: High-Sensitivity Mouse 5-HT ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with a proprietary matrix-suppression diluent. | Biotinylated recombinant monoclonal antibody (>95% pure) + Streptavidin-HRP conjugate (≥500 U/mg). |
| Key Advantage | Exceptional Value & Proven Reliability (>99% success rate). Employs a simultaneous incubation for efficiency. The specialized diluent minimizes background from hemoglobin and lipids, ensuring robust performance. | Ultimate Sensitivity & Matrix Immunity. The advanced biotin-streptavidin system directly neutralizes matrix effects, delivering superior signal-to-noise for the most demanding applications. |
| Price Advantage | Retail: $120. Bulk: <$100. A low-cost, high-quality alternative offering savings compared to premium brands. | Significantly more cost-effective than comparable high-sensitivity kits from leading suppliers, without sacrificing performance. |
| Our Selection Advice | Ideal for routine neuroscience & behavioral research. | Recommended for precious samples (e.g., micro-dissected brain regions) or detecting subtle physiological changes. |
Unbeatable Value Proposition
Why pay premium prices? Yanda Bio’s Mouse 5-Hydroxytryptamine (5-HT) kits are part of our commitment to providing cost-effective, high-performance research tools through efficient in-house production. Our direct-to-researcher model eliminates distributor markups, allowing us to offer:
- Superior Pricing: Obtain reliable data at a low-cost point, with bulk options under $100.
- Comparable Performance: Our kits are rigorously validated to deliver sensitivity and specificity on par with leading international brands.
- Included Value: Enjoy free expert testing services and comprehensive support often offered as expensive add-ons elsewhere.
Complimentary Expert Testing Service
Maximize efficiency and ensure results with our professional service, included with every purchase.
- Rapid Processing: Analysis begins within 24 hours of sample receipt.
- Complete Reporting: Receive a full data package in ~5 business days, including raw OD values, concentration tables, and a standard curve.
Our Commitment to Your Research
- Publication Reward: Cite this kit with a link to our site in your publication, and receive a free standard ELISA kit.
- 100% Satisfaction Guarantee: We promise performance with an unconditional replacement or refund.
- 24-Hour Support: Get a proposed solution within one business day.
Assay Principle & Specifications
Detection Principle: This sandwich ELISA captures mouse 5-HT between an immobilized antibody and an HRP-conjugated detection antibody. The TMB substrate reaction, measured at 450 nm, yields a signal proportional to serotonin concentration.
Performance Specifications:
- Sensitivity: < 1.0 ng/mL.
- Detection Range: 1.0 – 480 ng/mL.
- Accuracy: R ≥ 0.9900.
- Precision: Intra- & Inter-assay CV < 15%.
- Specificity: High specificity with minimal cross-reactivity.
- Storage: 2-8°C; 6-month shelf life.
Sample Preparation: For brain or intestinal tissue, use appropriate homogenization buffers. For serum/plasma, centrifuge at 3000×g for 10-30 mins. Aliquot and store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. A 1:5 dilution is typically required for liquid samples.
Streamlined Assay Protocol
- Add 50 µL of standards or prepared samples to the pre-coated wells.
- Add 100 µL HRP-conjugated Detection Antibody (except blank). Incubate at 37°C for 60 min.
- Wash the microplate 5 times thoroughly.
- Add 50 µL each of Substrate A & B. Incubate at 37°C for 15 min (dark).
- Add 50 µL Stop Solution. Read absorbance at 450 nm within 15 min.
Ordering & Custom Services
| Option | Description | Details |
|---|---|---|
| Tier 1: Standard Kit | Low-cost, reliable solution for common research. | Retail: $120. Bulk: <$100 (quote). |
| Tier 2: High-Sensitivity Kit | Cost-effective premium performance. | Contact for manufacturer price. |
| Expert Testing Service | We run the assay for you. | Free with kit purchase. |
| Custom ELISA Development | Tailored solutions. | Project-based quote. |
Ready to buy a high-quality, low-cost Mouse 5-HT ELISA Kit? Contact Yanda Bio for pricing or a custom quote.
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Research Applications & FAQ
Primary Applications (RUO): Depression & anxiety research, neurodegenerative disease studies (e.g., Alzheimer’s), gastrointestinal physiology (IBS research), sleep/wake cycle studies, and psychopharmacology (drug mechanism of action).
Q: Why is serotonin (5-HT) an important research target?
A: 5-HT is a key neurotransmitter regulating mood, cognition, and numerous peripheral functions. Its dysregulation is implicated in major depression, anxiety disorders, migraines, and GI disorders, making it a central biomarker in biomedical research.
Q: How do your prices compare to other major brands?
A: Through efficient in-house production, we offer Mouse 5-Hydroxytryptamine (5-HT) kits that are significantly more cost-effective than comparable products from leading international suppliers, providing exceptional value without compromising data quality.
Q: Can this kit be used for rat samples?
A: It is validated for mouse samples. For rat samples, please contact us about our custom service for cross-reactivity validation or species-specific development.
Q: How do I claim the free publication kit?
A: Send us the citation (DOI) of your article citing “Mouse 5-Hydroxytryptamine (5-HT) ELISA Kit (Yanda Bio)” with a link to our product page.
Manufacturer’s Note
For Research Use Only (RUO). Not for diagnostic use.
