Product Overview: Why Measure Porcine IL‑28B?
Interleukin 28B (IL‑28B), also known as interferon‑λ3 (IFN‑λ3), is a type III interferon that plays a critical role in antiviral immunity, particularly against hepatitis C virus and other RNA viruses. In swine, IL‑28B is involved in host defence against porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza, and other viral pathogens. Accurate quantification of Porcine IL‑28B is essential for:
- Swine virology and vaccine efficacy studies
- Immunology of viral respiratory and reproductive diseases
- Development of immunomodulatory therapies
- Preclinical research using porcine models
This is a custom‑designed ELISA test kit developed specifically for porcine IL‑28B detection, validated for swine serum, plasma, and tissue culture samples.
Why Yanda Bio is Your Trusted ELISA Test Kit Manufacturer & elisa diagnostic kit supplier
| EEAT Factor | Our Commitment |
|---|---|
| Experience | 6,000+ factors developed; 60+ university collaborations; years of expertise in cytokine and custom assay development. |
| Expertise | Proprietary antibody pairs validated for porcine IL‑28B; sandwich ELISA design ensures high specificity and minimal cross‑reactivity with other swine interleukins (IL‑28A, IL‑29, etc.). |
| Authoritativeness | Custom kits are developed under ISO‑aligned processes; batch‑to‑batch consistency; used in academic and industrial research. |
| Trustworthiness | Rapid custom development (3–7 days); transparent specifications; unconditional quality guarantee; free shipping on 3+ kits. |
Superior Quality Metrics
- Intra‑assay CV < 5 % — excellent within‑run precision
- Inter‑assay CV < 8 % — outstanding run‑to‑run consistency
- High specificity — no cross‑reactivity with porcine IL‑1, IL‑6, TNF‑α, or type I/II interferons
Kit Components: What’s Inside (96T Configuration)
| Component | Quantity |
|---|---|
| Microplate (Pre‑coated) | 8 wells × 12 strips |
| Standards (lyophilised or liquid) | 0.3 mL × 6 tubes (or as customised) |
| Sample Diluent | 6 mL |
| HRP‑Conjugated Detection Antibody | 6 mL |
| 20× Wash Buffer | 25 mL |
| Substrate A | 6 mL |
| Substrate B | 6 mL |
| Stop Solution | 6 mL |
| Plate Sealer | 2 sheets |
| Manual | 1 copy |
Other configurations (48T, bulk) available on request.
Assay Principle: Sandwich ELISA
This kit employs a solid‑phase sandwich enzyme‑linked immunosorbent assay. The microplate is pre‑coated with a monoclonal antibody specific for Porcine IL‑28B. Standards and samples are added, allowing IL‑28B to bind to the immobilised antibody. After washing, a Horseradish Peroxidase (HRP)‑conjugated detection antibody is added, forming an antibody‑antigen‑antibody “sandwich”. Following a second wash, TMB substrate is added. The HRP enzyme catalyses a blue colour that turns yellow upon acidification. The optical density (OD) at 450 nm is directly proportional to the IL‑28B concentration in the sample.
Sample Dilution Note: For serum or plasma, samples are pre‑diluted 5‑fold during the procedure (10 μL sample + 40 μL diluent). Multiply final results by 5.
Sample Collection & Preparation
| Sample Type | Collection & Processing |
|---|---|
| Serum | Use pyrogen/endotoxin‑free tubes. Avoid cell stimulation. Centrifuge at 3000 rpm for 10 minutes. |
| Plasma | Use EDTA, citrate or heparin anticoagulant. Centrifuge at 3000 rpm for 30 minutes. |
| Cell Supernatant | Centrifuge at 3000 rpm for 10 minutes to remove particles. |
| Tissue Homogenate | Homogenise tissue in PBS (1:9 w/v), centrifuge at 5000×g for 10 minutes at 2–8 °C. Collect supernatant. |
| Storage | Extract as soon as possible. Store at –20 °C (≤ 1 month) or –80 °C (≤ 2 months). Avoid repeated freeze‑thaw cycles. |
Important: Do not use samples containing sodium azide (NaN₃), as it inhibits HRP activity.
Materials Required But Not Provided
- Microplate reader (450 nm)
- Precision pipettes and tips (0.5–10 μL, 2–20 μL, 20–200 μL, 200–1000 μL)
- 37 °C incubator or water bath
- Distilled or deionised water
Procedure Notes & Important Tips
- Bring all reagents to room temperature before use. Concentrated wash buffer may crystallise; dissolve crystals in a warm water bath.
- Unused strips must be resealed with desiccant and stored at 2–8 °C.
- Standard dilution: Prepare serial dilutions of the stock standard according to the lot‑specific datasheet.
- Sample addition: Add 40 μL sample diluent to each sample well, then 10 μL sample. Mix gently without touching the well bottom.
- Incubation: Use the plate sealer and incubate at 37 °C for 30 minutes (first incubation).
- Washing: After each incubation, discard liquid, fill wells with wash buffer, stand for 30 seconds, then discard. Repeat 5 times. Incomplete washing may cause false positives.
- Substrate: Add Substrate A then B. Incubate in the dark at 37 °C for 10 minutes.
- Read OD: Add stop solution and read at 450 nm within 15 minutes.
For large numbers of samples, keep addition time within 5 minutes to avoid drift.
Detailed Assay Procedure
- Remove required strips from the foil pouch after 20 min equilibration. Reseal unused strips.
- Standards: Add 50 μL of each standard concentration to standard wells.
- Samples: Add 40 μL sample diluent to sample wells, then 10 μL test sample. Blank wells: leave empty.
- Add 50 μL HRP‑conjugated detection antibody to all wells except blanks. Seal and incubate at 37 °C for 30 min.
- Aspirate, wash 5 times (manual or automatic washer).
- Add 50 μL Substrate A and 50 μL Substrate B to each well. Gently tap to mix. Incubate at 37 °C in the dark for 10 min.
- Add 50 μL stop solution. Read OD at 450 nm within 15 min.
Data Analysis
Plot standard curve: OD values (y‑axis) vs. standard concentrations (x‑axis) using linear regression (R ≥ 0.9900). Calculate sample concentrations from the curve. For serum/plasma samples, multiply by the dilution factor (5×). For other sample types, use the appropriate dilution factor as determined.
Performance Specifications (Typical)
| Parameter | Performance |
|---|---|
| Accuracy | Standard curve linear regression coefficient (R) ≥ 0.9900 |
| Sensitivity | Minimum detectable concentration < 10 pg/mL |
| Detection Range | 32 – 2000 pg/mL |
| Specificity | No significant cross‑reactivity with porcine IL‑1α, IL‑1β, IL‑6, TNF‑α, IFN‑γ, or other type III interferons (IL‑28A, IL‑29) |
| Intra‑assay CV | < 5 % |
| Inter‑assay CV | < 8 % |
| Storage | 2–8 °C, protect from light |
| Shelf Life | 6 months |
Pricing & Bulk Discounts
| Quantity | Price per Kit |
|---|---|
| 1–2 kits | Regular retail – contact us for quote |
| 3 or more kits | Wholesale price – standard kits available under $100 per kit |
| Custom orders | Quote based on volume and specifications |
Free shipping on all orders of three or more ELISA kits. All products are shipped with free door‑to‑door delivery (tax included).
Free sample testing service is available upon request – contact our sales team for details.
Related Products (Recommended for Immune Research)
Expand your cytokine profiling with these trusted ELISA kits from Yanda Bio:
- Human Interleukin 1α (IL-1α) ELISA Kit – for human inflammation studies
- Rabbit Interleukin 13 (IL-13) ELISA Kit – for Th2 immune response in rabbit models
- Porcine Interleukin 10 (IL-10) ELISA Kit – an anti‑inflammatory cytokine for swine immunology (available as custom order)
Using IL‑28B together with IL‑10 and other Th1/Th2 markers provides a comprehensive view of immune regulation in porcine models.
Why Choose Yanda Bio for Your Customed ELISA Test Kit Needs?
- Manufacturer direct – over 6,000 detectable targets, 60+ university partners
- Rapid custom development – 3–7 days turnaround for new targets or matrices
- Strict quality control – intra‑assay CV < 5 %, inter‑assay CV < 8 %
- Cost‑effective – regular kit wholesale price < $100 for volumes of 3+
- Free technical support – from sample collection to data analysis
- Free sample testing service – for bulk orders
As a leading ELISA test kit manufacturer and reliable elisa diagnostic kit supplier, Yanda Bio supports your research with high‑quality, affordable immunoassays across all major species, including porcine, human, mouse, rat, rabbit, monkey, horse, chicken, and more.
Disclaimer
Strict adherence to the protocol is required. Yanda Bio is not responsible for results obtained from deviations. This product is for research use only; not for clinical diagnosis or human use. The user assumes all responsibility for any consequences arising from misuse.
Ordering & Contact Information
To order this Customized Porcine Interleukin 28B (IL-28B) ELISA Kit (a Customed ELISA Test Kit), or to inquire about volume discounts, custom development, and format selection, please contact our sales team.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
