Our Product Tiers: Find Your Perfect Match
| Feature | Tier 1: Standard ELISA Kit (Cost-Effective) | Tier 2: High-Sensitivity ELISA Kit (Premium Performance) |
|---|---|---|
| Core Technology | Standard HRP-conjugated detection antibody. | Biotinylated recombinant mAb (>95% pure) + Streptavidin-HRP (≥500 U/mg). |
| Key Advantage | Optimized for value. Uses a specialized diluent to reduce background interference from hemoglobin and lipids in serum/plasma samples. | Maximized sensitivity & specificity. The biotin-streptavidin amplification system directly circumvents matrix interference, offering superior signal-to-noise. |
| Sample Protocol | Serum/Plasma requires a 5-fold dilution with the provided buffer for optimal performance. | Designed for minimal interference, offering robust performance across sample types. |
| Ideal For | High-throughput screening, studies with ample target concentration, and budget-conscious projects. | Detecting very low-abundance NELL1, working with challenging sample matrices, or requiring the highest data precision. |
| Price Point | Retail: $120. Bulk (<$100). | Higher. Production costs are ~2.3x higher due to premium reagents. Contact for quote. |
| Both tiers utilize the same reliable one-step sandwich ELISA principle for consistent, reproducible results. |
Assay Principle & Key Features
Principle: Both kits use a One-Step Sandwich ELISA. The target NELL1 in samples is captured between a pre-coated antibody and an HRP-conjugated detection antibody. After washing, TMB substrate produces a color change measurable at 450nm, with intensity proportional to NELL1 concentration.
Why Choose Yanda Bio?
- Source Manufacturer Prices: Eliminate middleman markups.
- Proven Reliability: Validated in collaborations with 70+ academic institutions.
- Flexible Custom Service: Tailor kits to your unique requirements.
- Free Sample Testing: Validate kit performance with your samples before purchase.
Kit Specifications & Components
Shared Performance Specifications
- Accuracy: R value ≥ 0.9900.
- Sensitivity: < 0.1 ng/mL.
- Detection Range: 0.1 – 8 ng/mL.
- Specificity: No significant cross-reactivity.
- Precision: Intra- & Inter-assay CV < 15%.
- Shelf Life: 6 months at 2-8°C.
Standard Curve & Contents
Standard Concentrations (S0-S5): 0, 0.5, 1, 2, 4, 8 ng/mL.
Shared Components (96T/48T):
- Pre-coated Microplate (8/4 strips)
- Standards (0.3mL x 6 tubes)
- Sample Diluent* (6mL/3mL) *Tier-specific formulation
- Detection Antibody* (10mL/5mL)
- 20X Wash Buffer (25mL/15mL)
- Substrate A & B (6mL/3mL each)
- Stop Solution (6mL/3mL)
- Sealers, Manual, Bag
Sample Handling & Workflow
Collection: Follow standard protocols for serum, plasma (EDTA/citrate/heparin), cell culture supernatant, or tissue homogenate. Centrifuge at 3000×g for clarification.
Storage: Aliquot and store at -20°C; avoid repeated freeze-thaw cycles.
General Procedure:
- Add 50µL of standards or diluted samples (10µL sample + 40µL diluent for Tier 1 serum/plasma) to wells.
- Add 100µL of the respective HRP Detection Antibody.
- Incubate at 37°C for 60 min.
- Wash plate 5x.
- Add TMB Substrate (A&B), incubate at 37°C for 15 min (dark).
- Add Stop Solution, read OD at 450 nm.
Calculation: Use the standard curve. For Tier 1 diluted samples, multiply result by 5.
Ordering, Services & Support
Select Your Tier:
- Tier 1 (Standard): Retail $120. Bulk orders (under $100) available.
- Tier 2 (High-Sensitivity): Contact for pricing.
Value-Added Services:
- Custom ELISA Development: Modify kits per your specific research design.
- Free Sample Testing Service: Let us run your samples and provide the data.
Ready to order your Human NELL1 ELISA Kit? Contact Yanda Bio today for pricing, technical consultation, or to start your free testing service.
Applications & FAQ
Research Applications (RUO): Bone biology, craniosynostosis research, cancer studies (osteosarcoma, prostate cancer), vascular biology, and tissue regeneration.
Q: How do I choose between Tier 1 and Tier 2?
A: Tier 1 is ideal for routine analysis where target levels are expected to be within range and cost is key. Tier 2 is for pushing detection limits, working with problematic samples, or demanding maximum assay robustness.
Q: Why is Tier 2 more expensive?
A: The high-purity biotinylated monoclonal antibodies and high-activity streptavidin-HRP are significantly more costly to produce but provide unmatched sensitivity and interference resistance.
Q: Are you the actual manufacturer?
A: Yes. As a factory-direct source, we control quality and cost, passing the savings and support directly to you.
Technical Support & Disclaimer
Our team provides comprehensive support. This product is for Research Use Only (RUO). Not for diagnostic use. Users are responsible for following the protocol and applicable regulations.
