Are you searching for high-sensitivity Human CC chemokine ligand 12 (CCL12) for sale with verified specificity? Yanda Bio offers a rigorously validated Sandwich ELISA kit that eliminates matrix interference while delivering reproducible data for immunology and cancer studies. As the direct manufacturer, we remove middlemen from your procurement path. When you buy Human CC chemokine ligand 12 (CCL12) online from us, you access Human CC chemokine ligand 12 (CCL12) with manufacturer price —no distributor markups, just professional-grade reagents and the option to buy Human CC chemokine ligand 12 (CCL12) in China with full documentation support for customs clearance.
Price Reference for Common Human ELISA Kits:
- Retail Price: ~$120 USD
- Bulk/Wholesale Price: Under $100 USD
Choose Your Optimal Assay Format
To meet diverse research needs, we provide two optimized kit tiers. Purchasing either tier includes our complimentary expert testing service.
| Feature | Tier 1: Standard CCL12 ELISA Kit | Tier 2: High-Sensitivity CCL12 ELISA Kit |
|---|---|---|
| Core Technology | Optimized one-step sandwich ELISA with proprietary dilution buffer. | Biotinylated recombinant mAb (>95% pure) + Streptavidin-HRP (≥500 U/mg). |
| Key Advantage | Excellent Value & Reliability. Uses a simultaneous incubation step and a specialized diluent to minimize background interference from hemoglobin and lipids, ensuring robust performance. | Ultimate Sensitivity & Specificity. The advanced biotin-streptavidin system effectively neutralizes matrix effects, delivering superior signal-to-noise for low-abundance targets. |
| Ideal For | Routine analysis in standard immunoassay workflows. | High-precision studies, low-concentration samples, or complex matrices. |
Why Partner with Yanda Bio?
We do more than sell kits. We operate a dedicated protein-analysis laboratory and supply chain under one roof. Researchers who require large-volume reproducibility or custom coating formats can rely on our capacity.
Exclusive Sampling Service – Turn Data into Results Fast
- Samples processed within 24 hours of arrival.
- Fastest turnaround: 5 working days to receive your full data package.
- Deliverables: Raw Data, Concentration Calculation Sheet, Standard Curve Graph.
When your team is overloaded, our lab becomes your remote bench.
Unmatched Target Coverage
With 6,000+ validated detection targets spanning immunology, oncology, neuroscience, metabolism, and infectious disease, we continuously onboard emerging research targets to support diverse analytical needs from gene expression to functional protein quantitation. Our workflow supports long-term collaborations with 70+ universities.
Two-Tier Assay Architecture – Choose Your Precision Level
We engineered two distinct reaction formats to match your sample complexity and publication requirements.
Tier 1: Rapid Single-Step Incubation
Simultaneous binding of antigen to solid-phase antibody and enzyme-labeled detection antibody. The proprietary diluent actively suppresses common interferents like hemoglobin and lipids, reducing background fluctuation and stabilizing the reaction at the optimal binding site. Ideal for routine screening of clean sample matrices.
Tier 2: Biotinylated Recombinant Monoclonal Amplification System
Employs a biotinylated recombinant monoclonal detection antibody (purity >95%) paired with a Streptavidin-HRP conjugate (activity ≥500 U/mg). This signal-amplification cascade directly avoids matrix effects and endogenous interference, delivering superior signal-to-noise ratios for low-abundance CCL12 detection in complex samples like tissue homogenates.
Free Testing Service: Purchase any Yanda Bio ELISA research kit and receive complimentary sample processing using the corresponding kit, saving your team hands-on time.
Technical Specifications
Detection Principle
Double-antibody one-step sandwich ELISA. The microplate is pre-coated with anti-human CCL12 capture antibody. Samples, standards, and HRP-conjugated detection antibody are sequentially added, incubated, and washed. TMB substrate develops a blue color catalyzed by HRP, terminated to yellow. Absorbance at 450nm is directly proportional to CCL12 concentration.
Assay Performance
- Detection Range: 10.0 – 800 pg/mL
- Sensitivity: < 10.0 pg/mL
- Accuracy: Standard curve linear regression correlation coefficient R ≥ 0.9900
- Specificity: No cross-reactivity with structurally related soluble analogs
- Precision: Intra- and inter-assay CV < 15%
- Storage: 2–8°C, protected from light and moisture
- Shelf Life: 6 months
Sample Collection and Storage
- Serum: Collect in endotoxin-free tubes; centrifuge at 3000 rpm for 10 min.
- Plasma: EDTA, citrate, or heparin anticoagulation; centrifuge at 3000 rpm for 30 min.
- Cell Supernatant: Centrifuge at 3000 rpm for 10 min.
- Tissue Homogenate: Homogenize in normal saline; centrifuge at 3000 rpm for 10 min.
- Storage: Aliquot and store at -20°C if not tested immediately. Avoid repeated freeze-thaw cycles. Thaw at room temperature and ensure complete homogenization.
Required Equipment (Not Provided)
Microplate reader (450nm), precision pipettes and tips (0.5-10 μL to 200-1000 μL), 37°C incubator.
Assay Protocol Summary
- Equilibrate the kit to room temperature for 20 minutes.
- Add 50 μL of standards or 10 μL sample + 40 μL sample diluent to assigned wells; leave blank wells empty.
- Add 100 μL of HRP-detection antibody to each well except blank. Seal and incubate at 37°C for 60 minutes.
- Wash 5 times with 350 μL diluted wash buffer per well.
- Add 50 μL each of Substrate A and B; incubate 15 minutes at 37°C in the dark.
- Add 50 μL Stop Solution; read OD at 450 nm within 15 minutes.
- Calculate concentration using the standard curve generated in Excel (concentration vs. OD).
Custom ELISA Kit Service & Citation Reward
Need a specific coating format, detection range, or species reactivity not in our catalog? Our custom ELISA kit design team delivers tailored solutions. Provide your target specifications and we manufacture a pilot batch.
Publication Incentive: If you cite Yanda Bio’s ELISA kit in your paper and link to our website, we will send you a free standard ELISA kit of your choice from our regular catalog. Your next experiment could be on us.
Our Commitment
Six Manufacturing Advantages
- Full portfolio, consistent quality – 6,000+ targets under ISO-standard workflow.
- High sensitivity, strong stability – Validated lot-to-lot consistency.
- Simple protocol, fast detection – Optimized steps minimize hands-on time.
- Manufacturer-direct pricing – Eliminate distribution layers.
- Ample inventory, rapid dispatch – Most orders ship within 1–2 business days.
- Comprehensive support – Free latest quotations, principle documentation, product instructions.
After-Sales Assurance
24-hour response to any technical or product inquiry. If our product does not match the stated specifications upon arrival, we offer unconditional return or replacement—no delays, no arguments. Our reputation depends on your experimental continuity.
Assay Principle & Specifications
Principle: This one-step sandwich ELISA captures CCL12 between a pre-coated antibody and an HRP-conjugated detection antibody. TMB substrate produces a colorimetric signal measured at 450 nm, proportional to CCL12 concentration.
Performance Specifications:
- Sensitivity: < 10.0 pg/mL.
- Detection Range: 10.0 – 800 pg/mL.
- Accuracy (Linearity): R ≥ 0.9900.
- Precision: Intra- & Inter-assay CV < 15%.
- Specificity: No significant cross-reactivity.
- Storage: 2-8°C; 6-month shelf life.
Sample Preparation: Process serum, plasma, or cell culture supernatant by centrifugation (3000×g for 10-30 mins). Aliquot and store samples at -20°C; avoid repeated freeze-thaw cycles. A 1:5 dilution is recommended for serum/plasma.
Streamlined Assay Workflow
- Add 50 µL of standards or diluted samples to wells.
- Add 100 µL HRP-conjugated Detection Antibody.
- Incubate at 37°C for 60 min.
- Wash plate 5 times.
- Add 50 µL each of Substrate A & B. Incubate at 37°C for 15 min (dark).
- Add 50 µL Stop Solution. Read OD at 450 nm within 15 min.
Calculation: Plot the standard curve (0, X, Y… pg/mL) and interpolate sample values. Multiply results by the dilution factor.
Related Metabolite ELISA Kit:
Human CCL18 ELISA Kit: For Immune Regulation & Fibrosis Research
Applications & FAQ
Research Applications (RUO): Inflammatory disease research (arthritis, atherosclerosis), cancer microenvironment studies, autoimmune disorder mechanisms, monocyte/macrophage migration studies, and immunotoxicology.
Q: What is the difference between CCL12 and MCP-1?
A: While both are monocyte chemoattractants, CCL12 (MCP-5) and CCL2 (MCP-1) are distinct genes with specific receptor interactions and biological contexts. Our kit is specific for human CCL12.
Q: How do I claim the free kit for publication?
A: Upon publication of your article citing “Human CCL12 ELISA Kit (Yanda Bio)” with a link to our product page, send us the citation to claim your complimentary kit.
Q: How can I search your 6,000+ assay portfolio?
A: Contact our technical support with your target name or research area. We provide rapid availability checks and recommendations.
Q: How do I buy this ELISA kit from China?
A: As the direct manufacturer in China, you can buy this Human CCL12 ELISA Kit online by contacting Yanda Bio. We handle all export documentation and global shipping.
Manufacturer’s Note
This product is for Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical use. Users are responsible for determining the kit’s suitability for their specific applications.
