Mouse 1,25-Dihydroxyvitamin D3 ELISA Kit for Bone, Immune & Metabolic Research
Yanda Bio’s Mouse 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of the biologically active form of Vitamin D in mouse serum, plasma, and other biological fluids. As the key hormonal regulator of calcium-phosphate homeostasis, bone metabolism, and immune modulation, precise measurement of 1,25(OH)2D3 is crucial for research in osteoporosis, autoimmune diseases, cancer, and renal physiology using mouse models.
As a factory-direct manufacturer with dedicated R&D facilities in China, we deliver premium quality at exceptional value. This Mouse 1,25(OH)2D3 ELISA Kit made in China is competitively priced at a retail price of $120, with bulk orders available for under $100. Our expertise is backed by a portfolio of over 6,000 assays and collaborations with 70+ universities worldwide.
Two Performance Tiers for Precision & Value
We offer two optimized formats to suit your specific sensitivity and budget requirements. Purchasing either tier includes our complimentary expert testing service.
FeatureTier 1: Standard Mouse 1,25(OH)2D3 ELISA KitTier 2: High-Sensitivity Mouse 1,25(OH)2D3 ELISA Kit
Core Technology Optimized one-step sandwich ELISA with a proprietary matrix-suppression diluent. Biotinylated recombinant monoclonal antibody (>95% pure) combined with Streptavidin-HRP conjugate (≥500 U/mg).
Key Advantage: Excellent Cost-Effectiveness & Reliability. Employs a simultaneous single-step incubation for efficiency. The specialized diluent reduces background interference from hemoglobin and lipids, ensuring stable performance. Maximum Sensitivity & Matrix Resistance. The advanced biotin-streptavidin amplification directly neutralizes matrix effects, delivering superior signal-to-noise for detecting low-abundance targets or challenging samples.
Ideal For Routine analysis in studies where 1,25(OH)2D3 concentrations are expected within standard ranges. Research demanding the highest sensitivity (<0.1 ng/mL), such as detecting subtle physiological changes or analyzing limited sample volumes.
Complimentary Expert Testing Service: Fast, Authoritative Results
Leverage our professional laboratory to save time and ensure optimal data quality.
- Rapid Turnaround: We initiate your assay within 24 hours of sample receipt, delivering a comprehensive data package in ~5 business days.
- Complete Report: Receive raw OD values, calculated concentration tables, and a standard curve graph.
- Validation Support: Perfect for verifying assay performance with your specific mouse models.
Our Commitment to Your Research Success
We build trust through actionable guarantees:
- Publication Reward: Cite this Mouse 1,25(OH)2D3 ELISA Kit with a link to our product page in your publication, and we will send you a complimentary standard ELISA kit (up to $120 value).
- 100% Satisfaction Guarantee: We promise performance with an unconditional replacement or refund policy.
- 24-Hour Support Pledge: Our team provides a proposed solution within 24 hours of any inquiry.
- Guardians of Your Science: We handle every sample with the precision and care your research deserves.
Assay Principle & Technical Specifications
Detection Principle: The kit utilizes a validated sandwich ELISA. Mouse 1,25(OH)2D3 is captured between an immobilized antibody and an HRP-conjugated detection antibody. The TMB substrate reaction, measured at 450 nm, yields a signal proportional to the analyte concentration.
Performance Specifications:
- Sensitivity: < 0.1 ng/mL.
- Detection Range: 0.1 – 4 ng/mL.
- Accuracy (Linearity): Correlation Coefficient R ≥ 0.9900.
- Precision: Intra- & Inter-assay CV < 15%.
- Specificity: No significant cross-reactivity with analogous metabolites.
- Storage: 2-8°C, protected from light; 6-month shelf life.
Sample Handling: For serum/plasma, collect using standard protocols. Centrifuge at 3000×g for 10-30 minutes to clarify. Aliquot and store at -20°C, avoiding repeated freeze-thaw cycles. A 1:5 dilution is typically required.
Streamlined Assay Workflow
- Add 50 µL of standards or prepared samples to wells.
- Add 100 µL HRP-conjugated Detection Antibody (except blank).
- Incubate at 37°C for 60 min.
- Wash the plate 5 times thoroughly.
- Add 50 µL each of Substrate A & B. Incubate at 37°C for 15 min (dark).
- Add 50 µL Stop Solution. Read absorbance at 450 nm within 15 min.
Ordering Information & Services
OptionDescriptionDetails
Tier 1: Standard Kit Purchase Reliable, value-driven in-lab solution. Retail: $120. Bulk: < $100 (quote required).
Tier 2: High-Sensitivity Kit Purchase Premium performance for maximum sensitivity. Contact for a competitive manufacturer’s price.
Expert Testing Service Full-service analysis and reporting. ~5-day turnaround. Free with kit purchase.
Custom ELISA Development: Tailored assays for unique research needs. Project-based quotation available.
Ready to buy the Mouse 1,25(OH)2D3 ELISA Kit? Contact Yanda Bio for pricing, quotes, or to start your free testing service.
Research Applications & Frequently Asked Questions (FAQ)
Primary Applications (RUO): Bone and mineral metabolism studies (osteoporosis, renal osteodystrophy), autoimmune disease research (multiple sclerosis, rheumatoid arthritis), cancer biology (prostate, colon), and vitamin D metabolic pathway analysis.
Q: Why is measuring active 1,25(OH)2D3 important?
A: While 25-OH-Vitamin D reflects nutritional status, 1,25(OH)2D3 is the hormonally active form that directly mediates biological effects on gene expression related to calcium absorption, immune function, and cell growth. Its measurement is key to understanding dynamic physiological regulation.
Q: How do I claim the free kit for a publication?
A: Upon publication of your article citing the “Mouse 1,25(OH)2D3 ELISA Kit (Yanda Bio)” with a link to our product page, send us the citation details (DOI). We will promptly ship your complimentary reward.
Q: What is the main advantage of the Tier 2 kit?
A: The biotin-streptavidin amplification system in Tier 2 provides significantly higher sensitivity and better resistance to sample matrix interference, making it essential for detecting very low physiological levels or working with challenging sample types like lipemic serum.
Q: Why choose Yanda Bio as your supplier from China?
A: As a direct manufacturer, we provide authentic manufacturer pricing, complete in-house quality control, direct access to technical experts, and a vast portfolio, ensuring you receive a reliable, high-value research tool without intermediary markups.
Manufacturer’s Note
This product is for Research Use Only (RUO). Not for diagnostic, therapeutic, or clinical procedures. Users are responsible for determining the kit’s suitability for their specific applications.
