Rat 3 β-HSD ELISA Kit Materials supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0 、15 、30 、60 、120 、240pg/mL
Product Overview: Targeting a Key Enzyme in Steroidogenesis
3β-Hydroxysteroid Dehydrogenase (3β-HSD) is a pivotal enzyme located in the endoplasmic reticulum and mitochondria, catalyzing an essential step in the biosynthesis of all active steroid hormones. Its function is crucial for the conversion of pregnenolone to progesterone, dehydroepiandrosterone (DHEA) to androstenedione, and other key metabolic pathways. Accurate quantification of Rat 3β-HSD is therefore fundamental for research in endocrinology, reproductive biology, adrenal function, and disorders of steroid metabolism.
Yanda Bio’s Rat 3β-HSD ELISA Kit provides researchers with a sensitive, specific, and reliable tool to study this critical enzyme. As an expert in ELISA Kit production, with a comprehensive portfolio exceeding 6,000 detectable factors and proven, long-term collaborations with over 60 academic institutions, we specialize in delivering high-performance, cost-effective rat ELISA Kit solutions for rigorous scientific investigation.
Superior Quality Meets Exceptional Affordability
The performance and reproducibility of an ELISA kit are directly linked to the quality of its antibodies and the optimization of its assay chemistry. Premium international kits often command high prices based on these critical components.
Our Value Commitment: At Yanda Bio, we integrate high-specificity, high-affinity antibodies that meet stringent performance criteria, ensuring core reagent quality on par with leading brands. Through our efficient ELISA Kit production process and streamlined distribution model, we deliver this premium performance within an accessible and affordable rat ELISA Kit framework. This makes our product a genuinely cheap ELISA Kit alternative for steroid research, offering significant savings compared to premium international suppliers without compromising on data quality.
Dual-Format Flexibility: Optimized for Every Research Scenario
To address the diverse needs of the scientific community, from routine screening to high-sensitivity analysis of limited samples, we offer this assay in two distinct, performance-optimized formats.
Format A: High-Value Standard Kit
Designed for robust, reliable, and economical quantification of 3β-HSD in standard research settings. This format utilizes a simplified one-step incubation to reduce hands-on time. It features a proprietary matrix-optimization diluent that effectively minimizes interference from hemoglobin, lipids, and other common sample constituents, ensuring excellent reproducibility and a low background. With extensive validation demonstrating a success rate greater than 99%, this is our recommended affordable solution for most applications.
- Typical Pricing: Approximately $120 (retail). Under $100 per kit for bulk, wholesale, or institutional purchase agreements.
Format B: Advanced High-Sensitivity Kit
Engineered for the most demanding research involving low-abundance enzyme expression, scarce or irreplaceable samples (e.g., fine-needle biopsies, small tissue sections), or complex biological matrices. This advanced system employs a biotinylated recombinant monoclonal antibody (purity >95%) in combination with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This powerful signal amplification system achieves superior sensitivity, directly mitigates matrix effects, and is designed to ensure success in critical, one-attempt experiments.
Selection Guide for Researchers:
- Choose Format A for cost-effective and reliable analysis in studies with sufficient sample volume.
- Choose Format B when your project requires the highest level of detection sensitivity, involves trace-level analysis, or must overcome challenging sample backgrounds with limited material.
Assay Methodology: Robust Sandwich ELISA Design
This kit employs a well-established sandwich enzyme-linked immunosorbent assay (ELISA) principle. A highly specific capture antibody for Rat 3β-HSD is immobilized onto the microplate. During incubation, the enzyme present in standards or samples binds to these coated antibodies. Following a wash step, a Horseradish Peroxidase (HRP)-conjugated detection antibody is added, forming an antibody-antigen-antibody “sandwich” complex. After another wash to remove any unbound conjugate, a TMB substrate solution is added. The HRP enzyme catalyzes the conversion of TMB to a blue product, which turns yellow upon addition of the stop solution. The intensity of this yellow color, measured at 450 nm, is directly proportional to the concentration of 3β-HSD in the original sample.
Comprehensive Support & Guarantees
- Direct Savings on Premium Components: Obtain research-grade kits featuring high-quality antibodies through our efficient cost-effective rat ELISA Kit supply model.
- Complimentary Expert Testing Service: Purchase any Yanda Bio ELISA kit and receive FREE expert-assisted testing. Provide your samples, and our specialists will generate the data for you.
- Publication Recognition Program: Cite our product and link to this page in your peer-reviewed publication to receive a free standard ELISA kit.
- 100% Performance & Satisfaction Guarantee: We stand firmly behind our products. If performance does not meet the specifications detailed herein, we offer an unconditional replacement or full refund.
- Responsive 24-Hour Technical Support: Our dedicated team provides expert consultation and aims to deliver a proposed solution within one business day.
- Tailored Custom Development: For specialized research targets, inquire about our comprehensive custom ELISA kit design and production services.
Detailed Performance Specifications
- Accuracy & Linearity: The standard curve demonstrates a linear regression correlation coefficient (R) of ≥ 0.9900.
- Precision: Both intra-assay (within-run) and inter-assay (run-to-run) Coefficients of Variation (CV) are less than 15%.
- Specificity: The assay shows no significant cross-reactivity with other related soluble enzymes or steroidogenic proteins.
- Sample Compatibility: Validated for use with rat serum, plasma (EDTA, citrate, heparin), cell lysates, and tissue homogenates.
- Kit Stability & Shelf Life: The complete kit is stable for 6 months from the date of manufacture when stored continuously at 2-8°C.
Ordering & Contact Information
To purchase this Rat 3 β-HSD ELISA Kit for sale, or to discuss volume discounts, specific project needs, and custom assay services, please contact our sales team.
