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Rat C-Type Natriuretic Peptide (CNP) ELISA Kit | Reliable Quantification of a Key Cardiovascular Regulator

Product Code: YD-R-CNP
Application: For the specific and quantitative detection of Rat C-Type Natriuretic Peptide (CNP) in serum, plasma, cell culture supernatants, and tissue homogenates.
Detection Range: 0.1 – 80 U/mL
Sensitivity: < 0.1 U/mL Sample Volume: 10 μL Total Assay Time: Approximately 1 hour 45 minutes Storage: 2-8°C, protect from light Format: 96-well pre-coated strip plate [Buy Rat C-type Natriuretic Peptide (CNP) ELISA Kit Online] | [Request a Quote for Bulk Orders & Custom Kits]

Rat C-Type Natriuretic Peptide (CNP) ELISA Kit supplied

Name96 determinations48 determinations
Microelisa stripplate12*8strips12*4strips
Standard0.3ml*6tubes0.3ml*6tubes
Sample Diluent6.0ml3.0ml
HRP-Conjugate reagent10.0ml5.0ml
20X Wash solution25ml15ml
Chromogen Solution A6.0ml3.0ml
Chromogen Solution B6.0ml3.0ml
Stop Solution6.0ml3.0ml
Closure platemembrane22
User manual11
Sealed bags11

Note: Standard (S0 → S5) concentration was followed by:0,5, 10,20,40,80 pg/ml

Introduction to C-Type Natriuretic Peptide (CNP)

C-Type Natriuretic Peptide (CNP) is a vital paracrine signaling molecule belonging to the natriuretic peptide family. Primarily produced by endothelial cells and in the central nervous system, CNP plays a crucial role in regulating vascular tone, endothelial health, bone growth, and cardiovascular homeostasis. Accurate measurement of Rat CNP levels is essential for research in hypertension, atherosclerosis, heart failure, angiogenesis, and skeletal development studies.

Yanda Bio’s Rat C-Type Natriuretic Peptide (CNP) ELISA Kit is meticulously optimized to deliver precise and consistent results for this important biomarker. As a specialized manufacturer with an extensive portfolio of over 6,000 detectable factors and active, long-term collaborations with more than 60 universities in China, we are dedicated to providing high-quality, cost-effective rat ELISA Kit solutions that empower scientific discovery globally.


Professional Grade Performance at an Affordable Price

The foundation of a dependable ELISA lies in the specificity of its antibodies and the robustness of its assay design. While premium international kits offer reliability, they often come at a significant cost premium.

Our Value Proposition: At Yanda Bio, we source and utilize high-affinity, species-specific antibodies that meet rigorous performance standards, comparable to those used in leading-brand kits. By leveraging our optimized, in-house ELISA Kit production capabilities and a direct-to-researcher model, we eliminate unnecessary markups. This allows us to offer a professional-grade, highly affordable rat ELISA Kit that delivers exceptional sensitivity and specificity, providing a genuinely cheap ELISA Kit alternative without compromising data integrity for cardiovascular and endocrine research.


Two Optimized Formats for Tailored Experimental Needs

To accommodate diverse research requirements—from high-throughput studies to the analysis of rare or limited samples—we offer this assay in two strategically designed formats.

Format A: Standard High-Efficiency Kit
Ideal for routine, reliable quantification of CNP in standard research settings. This format employs a streamlined one-step incubation protocol, significantly reducing hands-on time. A proprietary matrix optimization buffer is included to effectively mitigate interference from common sample components like hemoglobin and lipids, ensuring low background noise and excellent reproducibility. With a validation history demonstrating a success rate above 99%, this format stands as our most cost-effective and affordable solution.

  • Typical Pricing: Around $120 (retail). Wholesale prices start under $100 per kit for volume purchases.

Format B: Advanced High-Sensitivity Kit
Designed for the most challenging research scenarios, such as detecting very low peptide concentrations or analyzing irreplaceable or minimal sample volumes (e.g., micro-dissected tissue, precious longitudinal samples). This advanced system features a biotinylated recombinant monoclonal antibody (purity >95%) combined with a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This powerful signal amplification architecture maximizes detection limits, robustly neutralizes matrix effects, and is engineered to guarantee definitive results in pivotal, one-attempt experiments.

Selection Guide for Researchers:

  • Select Format A for robust and economical analysis in projects with sufficient sample availability.
  • Select Format B when your study demands ultimate sensitivity for trace-level detection, involves scarce or invaluable samples, or requires maximum assay robustness against complex biological backgrounds.

Assay Principle: Robust Sandwich ELISA Technology

This kit utilizes a sensitive sandwich enzyme-linked immunosorbent assay (ELISA) methodology. A monoclonal antibody specific for Rat CNP is pre-coated onto the microplate. During incubation, the CNP present in standards or samples binds to these immobilized capture antibodies. Following a wash step to remove unbound material, a Horseradish Peroxidase (HRP)-conjugated detection antibody is added, forming an antibody-antigen-antibody “sandwich” complex. After a subsequent wash, a TMB (3,3′,5,5′-Tetramethylbenzidine) substrate solution is added. The HRP enzyme catalyzes the conversion of TMB to a blue product, which turns yellow upon acidification with the stop solution. The intensity of this yellow color, measured spectrophotometrically at 450 nm, is directly proportional to the concentration of CNP in the original sample.


Commitment to Research Excellence & Customer Success

  • Direct Access to Quality: Source premium immunoassay components through our efficient, cost-effective rat ELISA Kit supply chain, achieving significant savings versus premium international brands.
  • Complimentary Expert-Assisted Testing: Purchase any Yanda Bio ELISA kit and qualify for FREE testing by our specialists. Send us your prepared samples, and we will perform the assay and return the validated data.
  • Publication Acknowledgment Program: Cite our product and link to this product page in your peer-reviewed manuscript to receive a complimentary standard ELISA kit of your choice.
  • 100% Satisfaction & Performance Guarantee: We stand firmly behind our承诺 with an unconditional policy: if the product does not perform as detailed in the specifications, you are eligible for a prompt replacement or full refund.
  • Responsive 24-Hour Technical Support: Our dedicated support team is committed to providing a detailed, actionable response to any technical or application inquiry within one business day.
  • Custom Assay Development Services: For targets not yet in our vast catalog, we offer comprehensive custom ELISA kit design, development, and production services tailored to your specific research needs.

Detailed Performance Specifications

  • Accuracy & Linearity: The standard curve exhibits a linear regression correlation coefficient (R) of ≥ 0.9900, confirming excellent dose-response accuracy.
  • Precision: Both intra-assay (within plate) and inter-assay (between plates; day-to-day) coefficients of variation (CV) are consistently below 15%.
  • Specificity: The assay demonstrates negligible cross-reactivity with other natriuretic peptides (ANP, BNP) or structurally similar compounds.
  • Sample Compatibility: Validated for use with rat serum, plasma (EDTA, citrate, heparin anticoagulants), cell culture supernatant, and tissue homogenates.
  • Kit Stability & Shelf Life: The complete kit is stable for 6 months from the date of manufacture when stored continuously at the recommended 2-8°C.

Ordering & Contact Information

To order this Rat C-Type Natriuretic Peptide (CNP) ELISA Kit for sale, or to inquire about volume pricing, specific project consultations, and custom service options, please contact our sales team and provided the test factor .

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