Rat 5-HT2/3/4R ELISA Kit Materials supplied
| Name | 96 determinations | 48 determinations |
| Microelisa stripplate | 12*8strips | 12*4strips |
| Standard | 0.3ml*6tubes | 0.3ml*6tubes |
| Sample Diluent | 6.0ml | 3.0ml |
| HRP-Conjugate reagent | 10.0ml | 5.0ml |
| 20X Wash solution | 25ml | 15ml |
| Chromogen Solution A | 6.0ml | 3.0ml |
| Chromogen Solution B | 6.0ml | 3.0ml |
| Stop Solution | 6.0ml | 3.0ml |
| Closure platemembrane | 2 | 2 |
| User manual | 1 | 1 |
| Sealed bags | 1 | 1 |
Note: Standard (S0 → S5) concentration was followed by:0 、15 、30 、60 、120 、240 pg/mL
Product Introduction: Targeting Critical Serotonin Receptor Subtypes
Serotonin (5-Hydroxytryptamine, 5-HT) receptors 2, 3, and 4 (5-HT2/3/4R) are pivotal G-protein coupled and ligand-gated ion channels involved in a vast array of physiological and neurological processes. Their functions span mood regulation, anxiety, cognition, gastrointestinal motility, and vascular tone. Dysregulation of these receptors is implicated in depression, schizophrenia, migraine, and irritable bowel syndrome. Simultaneous or selective quantification of Rat 5-HT2/3/4R levels provides invaluable insights for neuropharmacology, psychotropic drug development, and gastrointestinal neuroscience research.
Yanda Bio’s Rat 5-HT2/3/4R ELISA Kit is expertly designed to offer researchers a robust, multiplex-capable tool for these complex targets. As a professional manufacturer with a portfolio exceeding 6,000 detectable factors and active research partnerships with over 60 domestic universities, we deliver high-quality, cost-effective rat ELISA Kit solutions that meet the precise demands of advanced neurobiological and pharmacological studies.
Laboratory-Grade Precision at an Unbeatable Price Point
Accurate quantification of specific GPCRs like serotonin receptors requires antibodies with exceptional subtype specificity—a feature that often inflates the cost of premium kits. We bridge the gap between premium quality and practical affordability.
Our Value Proposition: We utilize high-specificity, cross-reactive or subtype-selective antibodies validated for accurate detection of the target 5-HT receptor subtypes, ensuring research-grade performance. Through our optimized ELISA Kit production process and efficient distribution, we integrate this specificity into a remarkably affordable rat ELISA Kit framework. This model delivers a genuinely low-price ELISA Kit alternative, making critical receptor studies accessible without sacrificing the reliability and precision required for publication-ready data, at a fraction of the cost of leading international brands.
Assay Design Expertise: While this kit employs our highly sensitive sandwich ELISA format, our technical team can also develop assays using competitive ELISA or other methodologies. Contact us to tailor the perfect solution for your specific receptor target or application.
Dual-Format Flexibility: Choose Your Level of Sensitivity
To accommodate diverse research scenarios—from general screening to the analysis of low-abundance receptors in limited samples—we offer this comprehensive assay in two performance-based formats.
Format A: High-Value Standard Kit
Engineered for reliable, routine quantification of 5-HT2/3/4R expression. This kit features an efficient one-step incubation and a proprietary sample diluent. The diluent is specifically formulated to neutralize interference from high levels of lipids, hemoglobin, and other neural or systemic matrix components, ensuring a stable baseline and excellent reproducibility. With extensive validation supporting a >99% success rate, this is our most accessible and cheap ELISA Kit option.
- Typical Pricing: Approximately $120 (retail). Volume discounts bring the price below $100 per kit.
Format B: Advanced High-Sensitivity Kit
Purpose-built for high-stakes research involving trace receptor expression, scarce samples (e.g., specific brain nuclei micro-punches), or complex tissue lysates. This system leverages a biotinylated recombinant monoclonal antibody (purity >95%) and a high-activity Streptavidin-HRP conjugate (≥500 U/mg). This powerful signal amplification cascade maximizes the detection limit, effectively circumvents challenging matrix effects, and is engineered for guaranteed success in critical, one-attempt experiments with irreplaceable material.
Selection Guide for Optimal Results:
- Choose Format A for robust, economical analysis in studies with sufficient sample availability, such as whole tissue analysis or cell culture models.
- Choose Format B when your project demands the highest sensitivity for low-abundance targets, involves micro-dissected or otherwise limited samples, or requires maximum assay robustness.
Assay Principle: Specific Sandwich ELISA Technology
This kit operates on the robust sandwich enzyme-linked immunosorbent assay (ELISA) principle. The microplate is pre-coated with a capture antibody designed for specific interaction with Rat 5-HT2/3/4 receptor proteins. Standards and prepared samples are added, allowing target receptors to bind. After washing, an HRP-conjugated detection antibody is added, forming an immobilized “antibody-antigen-antibody” complex. Following a second wash, TMB substrate is added. The enzymatic reaction produces a blue color, which turns yellow upon stopping. The optical density (OD) at 450 nm is directly proportional to the concentration of the target serotonin receptors in the sample.
Why Choose Yanda Bio for Your Neuroresearch?
- Unmatched Value for Premium Assays: Access sophisticated multi-receptor detection technology through our efficient, cost-effective rat ELISA Kit supply model.
- Complimentary Expert Analysis: Purchase any Yanda Bio ELISA kit and receive FREE testing by our specialists. We generate reliable data from your provided samples.
- Publication Support Incentive: Cite our kit and link to our product page in your peer-reviewed article to receive a free standard ELISA kit.
- 100% Satisfaction & Performance Guarantee: We trust our products. If performance deviates from the published specifications, we offer a no-questions-asked replacement or full refund.
- Dedicated 24-Hour Technical Support: Receive expert consultation and a targeted solution proposal within one business day.
- Custom Assay Development: For novel targets or specific research needs beyond our catalog, we provide comprehensive custom ELISA kit services.
Detailed Performance Specifications
- Accuracy & Linearity: The standard curve demonstrates a linear regression correlation coefficient (R) of ≥ 0.9900.
- Precision: Both intra-assay and inter-assay Coefficients of Variation (CV) are < 15%.
- Specificity: The assay shows minimal cross-reactivity with other non-target serotonin receptor subtypes, ensuring specific detection.
- Sample Compatibility: Validated for rat serum, plasma, tissue homogenates (e.g., brain, intestine), and cell membrane fractions.
- Kit Stability & Shelf Life: The complete kit is stable for 6 months when stored continuously at 2-8°C.
Ordering & Contact Information
To purchase this Rat 5-HT2/3/4R ELISA Kit for sale, or to discuss bulk pricing, specific validation data, and custom assay services, please contact our sales team.
