Why Measure Rat Adiponectin?
Adiponectin (ADPN) is an adipokine secreted exclusively by adipose tissue. It plays a central role in glucose homeostasis, lipid metabolism, and insulin sensitivity. In rodent models, low adiponectin levels are associated with obesity, type 2 diabetes, insulin resistance, and cardiovascular disease. Accurate quantification of Rat Adiponectin is essential for:
- Metabolic syndrome and diabetes research
- Obesity and insulin resistance studies
- Cardiovascular and inflammatory disease models
- Preclinical drug efficacy evaluation
Key biological functions of adiponectin:
- Improves insulin sensitivity – enhances glucose uptake in muscle and liver.
- Anti‑inflammatory – suppresses pro‑inflammatory cytokines.
- Regulates lipid metabolism – promotes fatty acid oxidation and reduces hepatic steatosis.
- Cardiovascular protection – reduces risk of atherosclerosis and coronary events.
Maintaining adequate adiponectin levels through physical activity and healthy diet is critical for metabolic health.
Superior Quality Metrics
- Intra‑assay CV < 5 %
- Inter‑assay CV < 8 %
- High specificity – no cross‑reactivity with rat leptin, resistin, or other adipokines
Kit Components (96T & 48T)
| Component | 96‑Well | 48‑Well |
|---|---|---|
| Pre‑coated Microplate | 8 wells × 12 strips | 8 wells × 6 strips |
| Standards (6 vials) | 0.3 mL each | 0.3 mL each |
| Sample Diluent | 6 mL | 3 mL |
| HRP‑Conjugated Detection Antibody | 6 mL | 3 mL |
| 20× Wash Buffer | 25 mL | 15 mL |
| Substrate A | 6 mL | 3 mL |
| Substrate B | 6 mL | 3 mL |
| Stop Solution | 6 mL | 3 mL |
| Plate Sealer | 2 sheets | 2 sheets |
| Manual | 1 copy | 1 copy |
Assay Principle (Sandwich ELISA)
This kit uses a solid‑phase sandwich enzyme‑linked immunosorbent assay. The microplate is pre‑coated with a monoclonal antibody specific for rat adiponectin. Standards and samples are added, allowing adiponectin to bind to the immobilised antibody. After washing, an HRP‑conjugated detection antibody is added, forming an antibody‑antigen‑antibody “sandwich”. Following a second wash, TMB substrate is added. The HRP enzyme catalyses a blue colour that turns yellow after adding stop solution. The optical density (OD) at 450 nm is directly proportional to the adiponectin concentration.
Sample Dilution Note: Samples are pre‑diluted 5‑fold during the procedure – multiply final results by 5.
Sample Collection & Preparation
| Sample Type | Procedure |
|---|---|
| Serum | Use pyrogen/endotoxin‑free tubes. Centrifuge at 3000 rpm for 10 minutes. |
| Plasma | Use EDTA, citrate or heparin. Centrifuge at 3000 rpm for 30 minutes. |
| Cell Supernatant | Centrifuge at 3000 rpm for 10 minutes to remove particles. |
| Tissue Homogenate | Homogenise tissue in PBS (1:9 w/v), centrifuge at 5000×g for 10 min at 2–8 °C. |
| Storage | Aliquot and store at –20 °C (≤1 month) or –80 °C (≤2 months). Avoid repeated freeze‑thaw cycles. |
Materials Required (Not Provided)
- Microplate reader (450 nm)
- Precision pipettes (0.5–10 μL, 2–20 μL, 20–200 μL, 200–1000 μL)
- 37 °C incubator or water bath
Important Notes for Assay Procedure
- Bring all reagents to room temperature for 20 min before use.
- Unused strips must be resealed with desiccant and stored at 2–8 °C.
- The S0 standard (zero concentration) is the negative control. Multiply sample results by the dilution factor (5×).
- Strictly follow incubation times and volumes.
- Mix all liquid components thoroughly.
Reagent Preparation
20× Wash Buffer Dilution: Dilute 1 part 20× wash buffer with 19 parts distilled water to obtain 1× working wash buffer.
Washing Method
Manual: Aspirate liquid, fill each well with wash buffer, stand for 1 min, aspirate, and tap dry on absorbent paper. Repeat 5 times.
Automatic washer: Inject 350 μL per well, soak for 1 min, wash 5 times.
Assay Procedure (Step‑by‑Step)
- Equilibrate strips to room temperature for 20 min.
- Standard wells: add 50 μL of each standard concentration.
- Sample wells: add 10 μL sample + 40 μL sample diluent. Leave blank wells empty.
- Add 100 μL HRP‑conjugated detection antibody to all wells except blanks. Seal and incubate at 37 °C for 60 min.
- Aspirate and wash 5 times.
- Add 50 μL Substrate A + 50 μL Substrate B to each well. Incubate at 37 °C in the dark for 15 min.
- Add 50 μL stop solution. Measure OD at 450 nm within 15 min.
Data Analysis
Plot standard curve: OD values (y‑axis) vs. standard concentrations (x‑axis) using linear regression (R ≥ 0.9900). Calculate sample concentrations from the curve and multiply by the dilution factor (5×).
Performance Specifications
| Parameter | Value |
|---|---|
| Accuracy (R) | ≥ 0.9900 |
| Sensitivity | < 1.0 μg/mL |
| Detection Range | 1.0 – 800 μg/mL |
| Specificity | No cross‑reactivity with rat leptin, resistin, or other adipokines |
| Intra‑assay CV | < 5 % |
| Inter‑assay CV | < 8 % |
Pricing & Shipping
| Quantity | Price per Kit |
|---|---|
| 1–2 kits | $120 (retail) |
| 3+ kits | Wholesale price – contact for quote |
Free shipping on orders of three or more ELISA kits.
For the most competitive adiponectin elisa kit price, buy directly from the manufacturer – no middlemen.
Related Products (Recommended)
To expand your metabolic research, consider these complementary kits (available from Yanda Bio):
- Human Adiponectin ELISA Kit – for clinical and translational studies
- Mouse Adiponectin ELISA Kit – for murine metabolic disease models
Using rat, mouse and human adiponectin kits together allows cross‑species comparisons and validates findings across models.
Why Choose Yanda Bio?
- Manufacturer direct – over 6,000 targets, 60+ university partners
- Rapid custom development – 3–7 days turnaround
- Strict quality control – CV < 5 % intra‑assay, < 8 % inter‑assay
- Cost‑effective – premium performance at 40–60 % lower cost than leading brands
- Free shipping on 3+ kits
Disclaimer
This product is for research use only. Not for diagnostic or therapeutic use. Strict adherence to the protocol is required; Yanda Bio is not responsible for results obtained from deviations.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
