Yanda Bio introduces its ready-to-use Horse IL-1β ELISA kit, specifically developed for the quantitative measurement of interleukin-1β in equine serum, plasma, cell culture supernatant, and tissue homogenates. As a manufacturer with over 6,000 ELISA targets validated across human, mouse, rat, horse, rabbit, canine, bovine, chicken, duck, sheep, monkey, fish, insect, plant, and microbial species, we are trusted by more than 60 universities and research institutes in China. Every order is backed by manufacturer‑direct quality and a standout offer: buy any 3 kits and enjoy free shipping. Best of all, when you purchase this Horse Interleukin-1β ELISA kit, you unlock a complimentary elisa testing service — our professional lab will run the assay for you, record the entire process on video, and deliver all raw data and calculated results, completely free of charge. We also provide specialized immunogenicity elisa testing services to support equine vaccine and biologic development programs.
Why Interleukin-1β Matters in Equine Health
Interleukin-1β (IL-1β) is a master pro‑inflammatory cytokine produced predominantly by activated monocytes and macrophages. In horses, elevated IL-1β levels are strongly associated with a range of debilitating inflammatory conditions, including acute laminitis, osteoarthritis, septic arthritis, endotoxemia, and systemic inflammatory response syndrome (SIRS). Quantifying IL-1β with a reliable Horse IL-1β ELISA kit allows researchers and veterinary scientists to monitor disease progression, evaluate the efficacy of novel anti‑inflammatory therapies, and study the intricate cytokine networks involved in equine innate immunity. The growing interest in equine‑specific biomarkers makes a sensitive, species‑validated assay indispensable for translational and preclinical equine research.
Your Kit Comes with a Free, Fully Managed ELISA Testing Service
Yanda Bio operates a fully equipped professional ELISA laboratory. When you order this kit, you don’t just receive the reagents — you also gain access to our free elisa testing service. Simply ship your prepared equine samples to us, and our experienced technicians will handle the entire workflow:
- Precise sample processing and plate layout according to the kit protocol
- Automated or manual washing, incubation, and detection steps
- Complete video recording of the experiment so you can review every pipetting step
- Delivery of the raw OD values, standard curve, and final calculated concentrations in a ready‑to‑use Excel format
This hands‑free approach eliminates the need for in‑house optimization, reduces instrumentation burden, and guarantees expert‑level reproducibility. Additionally, for equine vaccine and therapeutic protein studies, Yanda Bio offers standalone immunogenicity elisa testing services that measure anti‑drug antibody responses or cytokine release profiles under GLP‑like documentation. Whether you need a single plate analyzed or a large‑scale immunogenicity screening, our elisa testing service infrastructure is built to accelerate your research timeline.
How the Assay Works – One‑Step Sandwich Principle
This Horse IL-1β ELISA kit is based on a quantitative one‑step double‑antibody sandwich technique. The microplate wells come pre‑coated with a high‑affinity capture antibody specific for equine IL-1β. During the assay, standards and samples are pipetted into the wells, where IL-1β binds to the immobilized antibody. An HRP‑conjugated detection antibody is added simultaneously, forming a stable antibody‑antigen‑enzyme sandwich in a single 60‑minute incubation. After thorough washing to remove unbound material, the TMB substrate is introduced. The HRP catalyzes a colorimetric reaction — first producing a blue color, which turns yellow upon addition of the acidic stop solution. The intensity of the yellow color, measured at 450 nm, is directly proportional to the concentration of equine IL-1β. A standard curve is generated using the provided calibrators, and sample concentrations are interpolated with ease.
Kit Components
| Component | 96‑Well Format | 48‑Well Format | Notes |
|---|---|---|---|
| Pre‑coated Microplate | 12 strips × 8 wells | 12 strips × 4 wells | Ready to use |
| Standard (S0–S5) | 0.3 mL × 6 tubes | 0.3 mL × 6 tubes | Concentrations: 0, 40, 80, 160, 320, 640 pg/mL |
| Sample Diluent | 6 mL | 3 mL | – |
| Detection Antibody‑HRP Conjugate | 10 mL | 5 mL | – |
| 20× Wash Buffer | 25 mL | 15 mL | Dilute before use |
| Substrate A | 6 mL | 3 mL | – |
| Substrate B | 6 mL | 3 mL | – |
| Stop Solution | 6 mL | 3 mL | – |
| Plate Sealers | 2 sheets | 2 sheets | – |
| Instruction Manual | 1 copy | 1 copy | – |
| Self‑sealing Bag | 1 piece | 1 piece | For unused strip storage |
Standard curve concentrations (S0–S5): 0, 40, 80, 160, 320, 640 pg/mL. The zero standard serves as the blank/negative control.
Assay Performance Characteristics
| Parameter | Specification |
|---|---|
| Detection Range | 1.0 – 640 pg/mL |
| Analytical Sensitivity | < 1.0 pg/mL |
| Accuracy | Standard curve linear regression R ≥ 0.9900 |
| Precision | Intra‑assay CV < 10% ; Inter‑assay CV < 15% |
| Specificity | Recognizes equine IL‑1β; no significant cross‑reactivity with structurally related soluble analogs |
| Sample Types | Serum, plasma (EDTA/citrate/heparin), cell culture supernatant, tissue homogenate |
| Shelf Life | 6 months when stored at 2–8°C and protected from light |
Sample Collection, Processing & Storage
Strict adherence to the following guidelines ensures reliable quantification and prevents HRP inhibition.
- Serum: Collect blood in endotoxin‑ and pyrogen‑free tubes without cell stimulation. Allow clotting, then centrifuge at 3000 rpm for 10 minutes to separate serum. Note regarding cell lysis buffers: if you are working with lysates rather than serum, please refer to our internal resource on the impact of cell lysis buffer components on ELISA performance .
- Plasma: Use EDTA, citrate, or heparin as anticoagulant. Mix, centrifuge at 3000 rpm for 30 minutes, and harvest the supernatant.
- Cell Culture Supernatant: Centrifuge at 3000 rpm for 10 minutes to remove particulates and debris.
- Tissue Homogenate: Homogenize equine tissue in an appropriate volume of normal saline. Centrifuge at 3000 rpm for 10 minutes and collect the supernatant.
- Storage: If not assayed immediately, aliquot samples and store at -20°C. Avoid repeated freeze‑thaw cycles. Thaw completely at room temperature and mix gently to ensure homogeneity.
Important: Samples are diluted 5‑fold during the assay procedure as per the standard protocol. Multiply the concentration obtained from the standard curve by 5 to obtain the actual sample concentration.
Assay Protocol Overview
Detailed step‑by‑step instructions are included with every kit. Below is a summary for quick reference.
- Equilibrate: Bring all reagents and the required number of coated strips to room temperature (20‑minute balance). Reseal unused strips immediately and return to 4°C.
- Prepare standards and samples: Pipette 50 µL of each standard into designated wells. For sample wells, add 10 µL of sample followed by 40 µL of sample diluent. Leave blank wells empty of standard/sample.
- One‑step incubation: Add 100 µL of HRP‑conjugated detection antibody to every well except the blank. Cover with a plate sealer and incubate at 37°C for 60 minutes.
- Wash: Aspirate the liquid completely. Wash each well five times with 1× wash buffer (prepare by diluting the 20× concentrate 1:20 with distilled water; warm to dissolve any crystals beforehand). Allow a 1‑minute soak per wash cycle, then blot dry on absorbent paper.
- Substrate reaction: Add 50 µL of Substrate A and 50 µL of Substrate B to each well. Incubate at 37°C in the dark for 15 minutes.
- Stop & Read: Add 50 µL of Stop Solution. Read the absorbance at 450 nm within 15 minutes. Construct the standard curve and calculate sample concentrations, remembering the 5× dilution factor.
Required Equipment (not supplied)
- Microplate reader with 450 nm filter
- Precision pipettes and tips (0.5‑10 µL, 2‑20 µL, 20‑200 µL, 200‑1000 µL)
- 37°C incubator or water bath
- Deionized/distilled water, graduated cylinders, absorbent paper
Key Operational Notes
- Wash buffer crystals are normal; completely redissolve by warming before dilution.
- Zero standard (S0) serves as the negative control/blank.
- Use calibrated pipettes and, for large sample numbers, a multichannel pipette to keep the pipetting step within 5 minutes.
- Run a fresh standard curve in duplicate with every experiment.
- For samples exceeding the highest standard, pre‑dilute further with sample diluent and multiply by the additional dilution factor.
- Plate sealers are single‑use to prevent cross‑contamination.
- Protect TMB substrate from light and treat all specimens as potentially biohazardous.
Expand Your Equine Cytokine Panel
This Horse IL-1β ELISA kit pairs effectively with other equine inflammatory markers. For multi‑cytokine profiling, consider these related products:
- Horse Interferon γ (IFN-γ) ELISA Kit – For the quantitative measurement of Horse Interferon γ (IFN-γ) levels in serum, plasma, cell culture supernatants, and tissue homogenates.
- Horse C-Reactive Protein (CRP) ELISA Kit – For the quantitative measurement of Horse C-Reactive Protein (CRP) levels in serum, plasma, cell culture supernatants, and tissue
(Please insert your internal links here.)
You may also explore our full portfolio of equine ELISA kits covering growth factors, chemokines, and metabolic hormones.
Disclaimer: This kit is for research use only. It is not intended for clinical diagnosis or veterinary therapeutic decision‑making. Follow the provided protocol precisely; any deviation is the sole responsibility of the experimenter. Yanda Bio shall not be liable for consequences arising from improper use.
Yanda Bio – From high‑quality equine ELISA kits to expert elisa testing service and immunogenicity elisa testing services, we power your equine research with precision and convenience.
