Yanda Bio presents its precise Horse Calcitonin (CT) ELISA kit, a ready-to-use enzyme immunoassay for quantifying equine calcitonin in serum, plasma, cell culture supernatant, and tissue homogenates. Backed by our catalog of over 6,000 validated ELISA targets across human, mouse, rat, horse, rabbit, canine, bovine, chicken, duck, sheep, monkey, fish, insect, plant, and microbial species, Yanda Bio serves more than 60 universities and research institutes in China. Every kit we ship is characterized by high reproducibility, strong specificity, and excellent sensitivity—with custom sensitivity options available upon request. Take advantage of free shipping when you order any 3 kits, and leverage our complimentary elisa testing service: our skilled laboratory team will run the assay on your samples at no charge, record the full procedure on video, and deliver all raw data. For projects requiring ultra‑low detection limits, inquire about our NHS‑biotin‑amplified ELISA kits. We also offer immunogenicity elisa testing services for equine drug and vaccine development. This kit is validated for equine samples exclusively and must not be used with human specimens.
Why Measure Equine Calcitonin?
Calcitonin (CT) is a 32‑amino‑acid peptide hormone secreted by the parafollicular C cells of the thyroid gland. It acts as a physiological antagonist to parathyroid hormone (PTH), lowering blood calcium and phosphate levels by inhibiting osteoclast‑mediated bone resorption and promoting renal calcium excretion. In horses, calcitonin plays a vital role in the fine‑tuning of calcium‑phosphorus balance, especially during growth, lactation, and skeletal remodeling. Clinically, measuring calcitonin in an equine calcitonin detection test is valuable for evaluating parathyroid gland function, investigating hypercalcemia of unknown origin, and studying bone metabolic disorders such as nutritional secondary hyperparathyroidism or equine osteoporosis. Because equine calcitonin differs structurally from human calcitonin, a species‑specific assay is essential. This horse CT quantitative ELISA ensures accurate and interference‑free quantification for veterinary research and preclinical studies. Relevant search phrases for this product include horse calcitonin hormone assay, equine thyroid C‑cell biomarker test, and equine calcium‑lowering hormone ELISA kit.
Free ELISA Testing Service – Your Samples, Our Expertise
When you purchase the Horse Calcitonin ELISA kit, you unlock more than a high‑performance reagent set. Yanda Bio operates a fully equipped professional laboratory and provides a free elisa testing service with every order. Simply ship your prepared equine samples to us, and our experienced technicians will handle the entire workflow:
- Thaw, aliquot, and load your samples following the kit protocol precisely
- Carry out plate setup, incubation, washing, and detection with expert precision
- Video‑record the complete experiment for full transparency and auditability
- Deliver the raw OD values, standard curve, and final calcitonin concentrations in a formatted Excel spreadsheet
This hands‑off approach is ideal for labs that want to conserve time and resources, lack specialized ELISA instrumentation, or need to process large sample batches rapidly. For research groups developing equine therapeutic antibodies or vaccines, Yanda Bio also provides immunogenicity elisa testing services that measure anti‑drug antibody responses and cytokine release profiles under rigorous documentation standards. Whether you need a single plate analyzed or a comprehensive elisa testing service for a multi‑timepoint study, our laboratory infrastructure is ready to accelerate your research timeline.
Assay Principle – One‑Step Sandwich ELISA
This horse calcitonin ELISA kit is based on a one‑step solid‑phase sandwich immunoassay. Microplate wells are pre‑coated with a capture antibody highly specific for equine calcitonin. During the assay, standards or samples are added simultaneously with an HRP‑conjugated detection antibody. In a single 60‑minute incubation step, calcitonin molecules are captured onto the solid phase and sandwiched with the enzyme‑labeled antibody. After thorough washing to remove unbound components, TMB substrate is introduced. The HRP catalyzes a chromogenic reaction—turning the solution blue, then yellow upon acidic stop—with the color intensity directly proportional to the calcitonin concentration. Absorbance is measured at 450 nm, and sample concentrations are calculated from a six‑point standard curve.
Kit Components
| Component | 96‑Well Format | 48‑Well Format | Remarks |
|---|---|---|---|
| Pre‑coated Microplate | 12 strips × 8 wells | 12 strips × 4 wells | Ready to use |
| Standard (S0–S5) | 0.3 mL × 6 tubes | 0.3 mL × 6 tubes | Concentrations: 0, 40, 80, 160, 320, 640 pg/mL |
| Sample Diluent | 6 mL | 3 mL | – |
| Detection Antibody‑HRP | 10 mL | 5 mL | – |
| 20× Wash Buffer | 25 mL | 15 mL | Dilute to 1× before use |
| Substrate A | 6 mL | 3 mL | – |
| Substrate B | 6 mL | 3 mL | – |
| Stop Solution | 6 mL | 3 mL | – |
| Plate Sealers | 2 sheets | 2 sheets | – |
| Instruction Manual | 1 copy | 1 copy | – |
| Self‑sealing Bag | 1 piece | 1 piece | For unused strip storage |
The zero standard (S0) serves as the blank/negative control.
Assay Performance Specifications
| Parameter | Specification |
|---|---|
| Detection Range | 0.1 – 16 ng/mL |
| Analytical Sensitivity | < 0.1 ng/mL |
| Accuracy | Standard curve linear regression R ≥ 0.9900 |
| Precision | Intra‑assay CV < 15%; Inter‑assay CV < 15% |
| Specificity | Recognizes equine calcitonin; no cross‑reactivity with structurally related soluble analogs |
| Sample Types Validated | Equine serum, plasma (EDTA/citrate/heparin), cell culture supernatant, tissue homogenate |
| Shelf Life | 6 months at 2–8°C, protected from light |
Need higher sensitivity? Yanda Bio offers custom sensitivity tuning and NHS‑biotin‑amplified ELISA formats. Contact our technical team to discuss your detection limit requirements.
Sample Collection & Preparation
Proper sample handling is essential for accurate calcitonin measurement.
- Serum: Collect blood in endotoxin‑ and pyrogen‑free tubes without cell stimulation. Allow clotting, then centrifuge at 3000 rpm for 10 min and separate serum.
- Plasma: Use EDTA, citrate, or heparin as anticoagulant. Centrifuge at 3000 rpm for 30 min and collect the supernatant.
- Cell Culture Supernatant: Spin at 3000 rpm for 10 min to remove particulates and debris.
- Tissue Homogenate: Homogenize equine thyroid or other tissue in an appropriate volume of normal saline, centrifuge at 3000 rpm for 10 min, and keep the supernatant.
- Storage: If not assayed immediately, aliquot samples and store at -20°C. Avoid repeated freeze‑thaw cycles. Thaw completely at room temperature and mix gently to ensure homogeneity.
Critical species note: This equine calcitonin ELISA has been validated exclusively for horse samples. It must not be applied to human specimens, as the antibodies and standards are optimized for equine calcitonin. Human calcitonin differs in amino acid sequence and will not be accurately detected by this kit. Any use with human samples falls outside the intended research purpose.
Condensed Assay Procedure
Complete step‑by‑step instructions are included with each kit. A quick‑reference summary is provided below.
- Equilibration: Allow all reagents and the required pre‑coated strips to reach room temperature (20 min). Reseal unused strips and store at 4°C.
- Loading: Pipette 50 µL of each standard into designated wells. For sample wells, add 10 µL sample + 40 µL sample diluent (final 5‑fold dilution). Leave blank wells empty of sample and standard.
- One‑Step Incubation: Add 100 µL HRP‑Detection Antibody to all wells except blanks. Cover with a plate sealer and incubate at 37°C for 60 min.
- Washing: Aspirate the liquid completely. Wash each well 5 times with 1× wash buffer (prepare by diluting 20× concentrate 1:20 with distilled water; dissolve any salt crystals by warming before dilution). Soak 1 min per wash, then blot dry on absorbent paper.
- Substrate Reaction: Add 50 µL Substrate A and 50 µL Substrate B to each well. Incubate at 37°C in the dark for 15 min.
- Stop & Read: Add 50 µL Stop Solution. Measure absorbance at 450 nm within 15 min. Construct the standard curve and calculate sample concentrations. Multiply the result by 5 to correct for the in‑assay dilution.
Required Equipment (not provided)
- Microplate reader with 450 nm filter
- Precision pipettes and tips (0.5‑10 µL, 2‑20 µL, 20‑200 µL, 200‑1000 µL)
- 37°C incubator or water bath
- Deionized/distilled water, graduated cylinders, absorbent paper
Important Technical Notes
- Concentrated wash buffer may form crystals at low storage temperature; dissolve completely by warming before dilution.
- The S0 zero standard is the blank/negative control.
- Use calibrated pipettes. For large sample numbers, a multichannel pipette is recommended to keep the loading step within 5 min.
- Run a fresh standard curve in duplicate with every experiment.
- For samples with OD exceeding the highest standard, pre‑dilute further with sample diluent and multiply by the additional dilution factor.
- Plate sealers are for single use only. Protect TMB substrate from light. Handle all biological specimens as potentially biohazardous.
Expand Your Equine Calcium Metabolism Panel
For a comprehensive evaluation of calcium homeostasis and bone remodeling in horses, pair this horse calcitonin ELISA kit with the following related assays:
- Horse Parathyroid Hormone (PTH) ELISA Kit – the primary physiological antagonist of calcitonin; use both together to study the PTH‑calcitonin regulatory axis in equine calcium balance.
- Horse Osteocalcin (Bone Gla Protein) ELISA Kit – a marker of osteoblast activity and bone formation, ideal for monitoring bone turnover during growth, training, or metabolic bone disease.
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Browse our full equine ELISA portfolio for additional bone metabolism markers, including vitamin D metabolites, bone‑specific alkaline phosphatase, and tartrate‑resistant acid phosphatase.
Why Researchers Rely on Yanda Bio
- Extensive species coverage: Over 6,000 validated ELISA targets spanning human, equine, rodent, livestock, companion animal, aquatic, and plant species.
- Proven performance: Our kits are characterized by high reproducibility, strong specificity, and excellent sensitivity—with the flexibility to accommodate custom sensitivity requirements.
- Academic trust: Long‑standing collaborations with more than 60 universities and research institutes in China.
- Manufacturer‑direct quality: In‑house development and production ensure consistent lot‑to‑lot performance, competitive pricing, and dedicated technical support.
- Free shipping: Order any 3 kits and receive complimentary delivery.
- Complimentary elisa testing service: We run the assay on your samples, record the experiment on video, and provide all raw data at no extra charge.
- High‑sensitivity custom options: For ultra‑low analyte concentrations, ask about our NHS‑biotin‑enhanced ELISA kits.
- Immunogenicity testing: Our immunogenicity elisa testing services provide robust anti‑drug antibody measurement for preclinical equine pharmaceutical and vaccine studies.
Disclaimer: This kit is for research use only. It is not intended for clinical diagnosis or veterinary therapeutic decision‑making. This equine calcitonin ELISA kit is validated for equine samples exclusively and must not be used with human specimens. Follow the provided protocol precisely; any deviations are the responsibility of the experimenter. Yanda Bio assumes no liability for consequences arising from improper use.
Yanda Bio – Delivering high‑quality equine ELISA kits, expert elisa testing service, and specialized immunogenicity elisa testing services to support your endocrine, metabolic, and skeletal research with reliable data and complete convenience.
