Yanda Bio presents its validated Horse IL-8 ELISA kit, a sensitive, one-step sandwich enzyme immunoassay designed to quantitatively measure equine interleukin-8 (IL-8/CXCL8) in serum, plasma, cell culture supernatant, and tissue homogenates. With a catalog of over 6,000 ELISA targets covering human, mouse, rat, horse, rabbit, canine, bovine, chicken, duck, sheep, monkey, fish, insect, plant, and microbial species, Yanda Bio is the trusted partner for more than 60 universities and research institutes in China. Every order is backed by manufacturer‑direct quality, free shipping on any three kits, and an unmatched service promise: our professional lab will run the ELISA for you at no extra cost, with full video documentation and complete raw data delivery. We also provide specialized immunogenicity elisa testing services for equine biologic development. Need to detect extremely low IL-8 concentrations? Ask us about our NHS‑biotin‑amplified ELISA format. *This equine IL-8 kit is for research use only and is not validated for human samples.*
Why Quantify Equine Interleukin-8?
Interleukin-8, also called CXCL8, is the most potent neutrophil chemoattractant in the horse. It drives directed migration and activation of neutrophils to sites of tissue injury and infection, making it a central player in acute and chronic inflammatory conditions. In equine medicine, elevated IL-8 levels are strongly linked to recurrent airway obstruction (RAO), exercise‑induced pulmonary hemorrhage, endotoxemia, ischemia‑reperfusion injury in the large colon, and endometritis. Quantifying IL-8 with an equine IL-8 ELISA kit allows researchers to monitor neutrophil‑driven inflammation, evaluate the efficacy of anti‑inflammatory or immunomodulatory therapies, and dissect chemokine networks in equine innate immunity. Whether you need a reliable horse interleukin-8 quantitative assay, a horse chemokine IL-8 test, or a robust equine neutrophil chemotactic factor ELISA, this kit delivers reproducible results for your veterinary and translational research.
Complimentary ELISA Testing Service – We Run the Assay for You
Your purchase includes far more than a set of reagents. Yanda Bio operates a fully equipped professional laboratory and provides a free elisa testing service with every kit. Simply ship your prepared equine samples to our facility, and our experienced technicians will:
- Thaw, aliquot, and load your samples according to the protocol
- Perform the entire plate setup, incubation, washing, and detection
- Video‑record the complete experiment for full transparency
- Compile the raw OD values, standard curve, and calculated IL-8 concentrations in a ready‑to‑use Excel file
This hands‑off approach eliminates the need for in‑house optimization and saves instrument time. It is ideal for high‑throughput projects or labs lacking specialized ELISA equipment. Additionally, for researchers developing equine vaccines or therapeutic antibodies, Yanda Bio offers immunogenicity elisa testing services to measure anti‑drug antibody responses and cytokine release profiles under rigorous documentation standards. Whether you need a single plate analyzed or a large‑scale immunogenicity screening, our elisa testing service infrastructure accelerates your research timeline while guaranteeing expert‑level reproducibility.
Assay Principle – One‑Step Sandwich ELISA
The horse IL-8 ELISA kit is built on a one‑step solid‑phase sandwich immunoassay. Microplate wells are pre‑coated with a high‑affinity capture antibody specific for equine IL-8. You add standards or samples together with an HRP‑conjugated detection antibody in a single pipetting step. During the 60‑minute incubation, IL-8 is simultaneously captured and labeled, forming an antibody‑antigen‑enzyme sandwich. After a rigorous wash to remove unbound material, the TMB substrate is added. The HRP catalyzes a colorimetric reaction — turning the solution blue, then yellow upon acidic stop — with intensity directly proportional to the IL-8 concentration. Absorbance is read at 450 nm, and sample concentrations are interpolated from the standard curve.
Kit Components
| Component | 96‑Well Format | 48‑Well Format | Remarks |
|---|---|---|---|
| Pre‑coated Microplate | 12 strips × 8 wells | 12 strips × 4 wells | Ready to use |
| Standard (S0–S5) | 0.3 mL × 6 tubes | 0.3 mL × 6 tubes | Concentrations: 0, 40, 80, 160, 320, 640 pg/mL |
| Sample Diluent | 6 mL | 3 mL | – |
| Detection Antibody‑HRP | 10 mL | 5 mL | – |
| 20× Wash Buffer | 25 mL | 15 mL | Dilute to 1× before use |
| Substrate A | 6 mL | 3 mL | – |
| Substrate B | 6 mL | 3 mL | – |
| Stop Solution | 6 mL | 3 mL | – |
| Plate Sealers | 2 sheets | 2 sheets | – |
| Instruction Manual | 1 copy | 1 copy | – |
| Self‑sealing Bag | 1 piece | 1 piece | For unused strip storage |
The S0 zero standard serves as the blank/negative control.
Performance Characteristics
| Parameter | Specification |
|---|---|
| Detection Range | 1.0 – 640 pg/mL |
| Analytical Sensitivity | < 1.0 pg/mL |
| Accuracy | Standard curve linear regression R ≥ 0.9900 |
| Precision | Intra‑assay CV < 5%; Inter‑assay CV < 10% |
| Specificity | Recognizes native equine IL‑8; no significant cross‑reactivity with structurally similar cytokines |
| Validated Sample Types | Equine serum, plasma (EDTA/citrate/heparin), cell culture supernatant, tissue homogenate |
| Shelf Life | 6 months when stored at 2–8°C and protected from light |
To better understand the sensitivity metrics and validation data behind our kits, read our complete guide: [How Is ELISA Kit Sensitivity Determined? A Complete Guide to Key Performance Indicators]
Sample Collection, Processing & Storage
Strict adherence to the following practices ensures accurate and reproducible IL-8 quantification.
- Serum: Collect blood in endotoxin‑ and pyrogen‑free tubes, allow clotting without agitation, and centrifuge at 3000 rpm for 10 minutes to separate serum.
- Plasma: Use EDTA, citrate, or heparin as anticoagulant. Centrifuge at 3000 rpm for 30 minutes and harvest the supernatant.
- Cell Culture Supernatant: Spin at 3000 rpm for 10 minutes to remove cells and debris.
- Tissue Homogenate: Homogenize equine tissue in an appropriate volume of normal saline, centrifuge at 3000 rpm for 10 minutes, and keep the supernatant.
- Storage: If not tested immediately, aliquot samples and store at -20°C. Avoid repeated freeze‑thaw cycles. Thaw fully at room temperature and mix gently before use.
Critical species note: This kit has been validated exclusively for equine samples. It must not be used with human specimens, as the antibodies and standards are optimized for horse IL-8 and will not provide reliable results on human material. Using this kit for human samples violates the intended use and may lead to erroneous data.
Assay Procedure at a Glance
Detailed step‑by‑step instructions are supplied with every kit. Below is a condensed overview.
- Equilibrate: Bring all reagents and the required number of pre‑coated strips to room temperature for 20 minutes. Reseal unused strips immediately and store at 4°C.
- Load Standards & Samples: Pipette 50 µL of each standard into designated wells. For sample wells, add 10 µL of sample followed by 40 µL of sample diluent (final 5‑fold dilution). Blank wells receive neither sample nor standard.
- One‑Step Incubation: Add 100 µL of HRP‑conjugated detection antibody to all wells except blanks. Cover with a plate sealer and incubate at 37°C for 60 minutes.
- Washing: Aspirate the liquid completely. Wash each well five times with 1× wash buffer (prepare by diluting the 20× concentrate 1:20 with distilled water; warm to dissolve any crystals beforehand). Soak for 1 minute per wash cycle, then blot dry on absorbent paper.
- Substrate Reaction: Add 50 µL of Substrate A and 50 µL of Substrate B to each well. Incubate in the dark at 37°C for 15 minutes.
- Stop & Read: Add 50 µL of Stop Solution. Read the absorbance at 450 nm within 15 minutes. Construct the standard curve and calculate sample concentrations. Remember to multiply the obtained value by 5 to account for the in‑assay dilution.
Required Equipment (not supplied)
- Microplate reader with 450 nm filter
- Precision pipettes and tips (0.5‑10 µL, 2‑20 µL, 20‑200 µL, 200‑1000 µL)
- 37°C incubator or water bath
- Deionized/distilled water, graduated cylinders, absorbent paper
Key Technical Notes
- Concentrated wash buffer may form crystals at low temperature; completely dissolve by warming before dilution.
- The S0 standard functions as the negative control/blank.
- Use calibrated pipettes and, for large sample numbers, a multichannel pipette to complete the pipetting step within 5 minutes.
- Include a duplicate standard curve with every experiment.
- If a sample’s OD exceeds the highest standard, pre‑dilute further with sample diluent and multiply by the additional dilution factor.
- Plate sealers are for single use only. Protect TMB from light. Treat all biological specimens as potentially biohazardous.
Expand Your Equine Inflammation Panel
For comprehensive profiling of equine inflammatory mediators, pair this horse IL-8 kit with:
- Horse Interleukin-6(IL-6) ELISA Kit – For quantitative measurement of interleukin-6 in equine serum, plasma, cell culture supernatant, and tissue homogenates.
- Horse Interleukin-1β (IL-1β) ELISA Kit – a master pro‑inflammatory cytokine elevated in equine septic arthritis, osteoarthritis, and acute laminitis.
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Explore our full range of equine ELISA kits covering TNF‑α, IL‑6, IL‑10, growth factors, and acute‑phase proteins to build a complete cytokine and chemokine profiling platform.
Why Yanda Bio Is the Right Choice for Equine ELISA
- Unrivaled product diversity: Over 6,000 ready‑to‑order ELISA kits spanning more than 15 species, from horse, human, and mouse to zebrafish, plant, and insect.
- Academic trust: Long‑term collaborative relationships with 60+ universities and research institutes in China, supporting impactful equine and veterinary studies.
- Manufacturer‑direct quality: In‑house development, production, and lot‑to‑lot consistency control deliver superior reproducibility at competitive prices.
- Free shipping: Order any 3 kits and enjoy complimentary shipping, reducing the logistical burden on your lab.
- Complimentary full‑service ELISA: Every kit purchase includes access to our elisa testing service — we run the assay, provide a video record, and send all data, completely free of charge.
- High‑sensitivity options: Need to quantify very low IL-8 levels? Contact us about our NHS‑biotin‑enhanced ELISA kits, which amplify signal through biotin‑streptavidin chemistry for improved detection limits.
- Dedicated immunogenicity testing: Beyond routine cytokine quantification, our immunogenicity elisa testing services support equine drug and vaccine development with robust anti‑drug antibody assays.
Disclaimer: This kit is for research use only. It is not intended for clinical diagnosis or veterinary therapeutic decision‑making. This equine IL-8 ELISA kit is validated for equine samples exclusively and must not be used on human specimens. Follow the provided protocol precisely; any deviation is the responsibility of the experimenter. Yanda Bio shall not be liable for consequences arising from improper use.
Yanda Bio – From high‑quality equine ELISA kits to expert elisa testing service and immunogenicity elisa testing services, we equip your equine inflammation research with precision, convenience, and complete data transparency.
